Our Gush Mintz have undergone a small revolution and are still super beautiful, even more than before. One of the plants was damaged during the main lining I broke a brunch and it was transformed into a very very beautiful LST plant, it could remain small but I wouldn't swear it has a truly spectacular shape. In this diary therefore you will find the plant worked without techniques that require cutting but only with a LST this time also on the main stem and very aggressive to give a beautiful shape. The comparison is the salt of the experience so the plant in the other diary will be worked very differently. We have started the Plagron fertilization program, we are in 100% organic configuration, the soil is recycled Promix + 1/3 fresh soil + 10% Perlite + RQS Mycorrhiza Mix (4 g in the mix, 1 g under the small fiber pot). We are administering // 1 ml/l Power Roots - 1 ml/l Pure Zym - 1 ml/l Sugar Royal - 3 ml/l Alga Grow https://plagron.com/en The doubts about the compatibility between Pure Zym and mycorrhizae have been dispelled, they can get along according to Plagron experts. If someone tells you that enzymes eat mycorrhizae, which in principle may seem possible, explain to them that it is not true. https://www.royalqueenseeds.it/growing/452-easy-roots-mix-di-micorrhize.html Try this strain, it's a top strain of the last few years very high thc level ------------------------------------------------------------------- https://www.zamnesia.io/it/11234-zamnesia-seeds-gush-mintz-automatic.html Brief description of Zamnesia // Gush Mintz Auto is a relaxing hybrid strain that derives from the original Gush Mintz and a high-performance ruderalis specimen. This cross produced a plant with 70% indica and 30% sativa genetics. Her parents have given her a range of interesting traits, including mint and candy flavors and a high THC content of 20%. Smoking these buds provides creative vibes, a sense of euphoria and a pleasant sensation from head to toe. In addition to its enticing flavors and powerful effects, Gush Mintz Auto produces large yields of compact buds in just 9–10 weeks from germination. Gush Mintz Auto is extremely easy to grow, with plants reaching an average height of 70cm indoors and 120cm outdoors, making her ideal for discreet grows or limited spaces both indoors and outdoors. This strain is well suited to LST (Low Stress Training) and performs best in a ScrOG setup (a technique that optimizes light exposure to maximize yields). Punching aromas and deeply relaxing effects As she grows, Gush Mintz Auto's aroma intensifies, releasing sweet and minty notes. This refreshing flavor profile really comes into its own when smoked, offering an inviting combination of minty freshness and sweetness. The effects are deeply relaxing, enveloping the body in a pleasant well-being without being too sedative. Gush Mintz Auto is perfect for those seeking creative inspiration, making writing, painting or music-making sessions smoother and more inspiring. At Zamnesia you can find the whole world of growing and much more. Take a tour around the site and you will find "all the best that nature has to offer" in various shapes and colors. The new strains are amazing and the old ones are no exception... p.s. for now zero Hermaphrodites, growers, can you say the same? --- // www.zamnesia.com

Hi everyone 🤗 This week is very strong growth nothing happens :-) Everything as usual, I think in 2-3 weeks you will move to the flower tent 😍. I wish you all a nice week, stay healthy 🙏🏻 and let it grow 🌱 You can buy the strain at : https://greenhouseseeds.nl/ Type: Wonder Pie ☝️🏼 Genetics: Wedding Cake x OG Kush 👍😍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Flower Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205 W 💡💡☝️🏼 Earth: Canna Bio ☝️🏼 Fertilizer: Canna Bio ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 6.0

Hot and some rainy days which has been great for the growth of the girls. They are flourishing everyday. Responding well to the Grow and CalMag nutrients too. You can tell that the one I had topped is slightly wider than the untouched one. Might experiment a bit more with the topped one to get more of a lollipop look. Still have issues with the Japanese Beatles but I've noticed them getting attracted to the flowers in the garden so hopefully they stick to those instead of gnawing on the leaves of the girls.

I welcome you from Russia my friends from different parts of the world. The end of 16 weeks has come. Plants already fully show their ripeness, and buds SAG from their weight. Soon I will be harvesting, as soon as I get the final dry product I will make the last update of this diary.

Whew! Miss Hypnotic continues to amaze me! She continues to be a light hog but LOVED THE CONDITIONS!! She has trichomes on the sugar leaves already! She smells beautiful alone and with the other girls! I'm posting this as a question but what advice do you guys have for taming her height 🤔 Edit 12/19-I figured out how to tame down her height! I took garden wire and high stress trained her. She's responding well so I'm happy too! No light burn/bleaching over here!

Hello my friendly growmies 👩‍🌾👨‍🌾🌲🌲, The #1 is my biggest plant ever, 100 cm, it's not a record lol, but for me it's tall 😊😊 The #2 start to turn purple, can't wait to see 💜🍌 Buds start to be formed and are biggest each days 🌲🌲 I'm totally in love with those cute ladies 🌲🌲🌲😁😁💜💜🍌🍌 I've continued to train a bit. 💦I give water each 3 days, 2.75l/plant Water + RQS Bio Flowering Booster Water + cannazym + Sugar Royal @PH6 RQS Bio Flowering Booster - Week 3 THICKER 0.6 ml/l BIGGER FLOWERS 0.8 ml/l The RQS liquid kit, look doing the job, not overfert or deficiency sign for the moment. Lamp @100% and start to be really close to the plant, but not burning sign for the moment. Thanks community for follow, likes, comments, always a pleasure 👩‍🌾👨‍🌾❤️🌲 Mars Hydro - TS1000 💡💡 https://www.mars-hydro.com/ts-1000-led-grow-light Mars Hydro - FC3000 💡💡 https://www.mars-hydro.com/fc-3000-samsung-lm301b-led-grow-light RQS - Fat Banana Auto 🍌🍌🌲🌲 https://www.royalqueenseeds.com/autoflowering-cannabis-seeds/487-fat-banana-automatic.html

Grow going well. Girls getting frosty.

Usually I start my diary after they all pop but I’m feeling lucky and especially green this round. After numerous failed attempts at getting auto seeds from various sources and not having them pop no matter what I did. I finally got in touch with THSeeds and told them that the Critical Hog auto they sent me were totally bunk and not one popped. They had Truenorth seedbank send me this bubblegum Within a week of my complaint. I’m hoping these are fresh vigorous seeds and after much touch up learning on seed popping Iv decided to go straight from the water cup to the medium which is Black Gold seedlings mix in the center 6 inches of my coco soil so to give them a easy start with no added nutes. So it begins.... Stay tuned fellow growers!

Day 8 Mon PH 5.7 EC 0.8 DLI /19h / PPFD / Water 23c Day 9 Thue Change the water and add nuts 10 L PH 5.2/5.8 EC 0.8/0.8 DLI /19h / PPFD / Water 25c Day 10 Wed I've confidently carried out defoliation on the lower branches of my autoflower plant. Despite the advice against it for autoflowers, I've decided to trust my intuition and follow my gut feeling. Time will tell the results. 😎 PH 6.3/5.8 EC 0.8 DLI /19h 17 PPFD / Water 22c Day 11 Thu PH 6.1/5.8 EC 0.8/0.9 DLI /19h 17 PPFD / Water 22c Day 13 Sat Add water to lower EC PH 5.8/ EC 1.0/0.8 DLI /19h / PPFD / Water 22c Day 14 Sun The plant was slow to grow, but now it's starting pre-flower early. Add water to lower EC PH 5.2/5.8 EC 0.9/0.6 DLI /19h / PPFD / Water 20c

Zbliżamy się do końca. Dziewczyny dostają już tylko wodę. Pojawiają się mleczne trichomy, czekam mniej więcej na stosunek mleczne-brazowe 80-20%. Gubią dużo liści (szczególnie ethos) , dziewczyny się zjadają :-). Ravenberry ma piękne jesienne kolory. Temperatura w dzien w tym tygodniu nie przekroczyla 25C w nocy 18-19C. wilgotnosc 40-50%

Starting to keep a close eye on the Trichomes. Still clear some cloudy. Stopped feeding, Starting to flush. It’s starting to smell amazing in here.

Start week 4 , Bio Grow 2 ml/L + Cal-Mag Xtra 2 ml/L ( Ph 6.6).

I stressed this lady out early accidentally , she bounced back as best as I permitted room for . She bared with me all the way until the end throughout this entire learning cycle . I’m deeply appreciative for the ease of grow & level of bounce back she had . It was the most impressive grow but it didn’t require rocket science to grow this either

They are really stretching their legs this week. The big Blueberry Muffin lady in the back corner, who didn’t get topped as much as the other plants, is really stretching! Bud development is showing and it smells so sweet! I have seen a thrip here and there, but a healthy Nuke ‘Em routine took care of them with no problem!

This week, Tart Pops got a serious upgrade — moved into a big 50L pot and loving the legroom! Roots were wrapping tight around the old pot, so it was time to stretch out and settle in. Gave her a solid flush to clear out any leftover salts before the big shift. She’s heading straight into flower mode in the tent after this — lights, camera, pistils! Expecting explosive growth in the next 7–10 days as she settles into the new digs and switches gears. Let the bloom begin! Next Up: Monitor transition stretch Light feeding after flush First signs of flower incoming…

The most fucked up hurry up scrog

Giving her banana peel tea everyday 100mls with a 200ml of water. Hairs are turning orange and now just waiting for them to fatten up.

Runtz from Zamnesia is came along from her tiny shape. Got a great clone and is also catching up. She's on about week 14 (the mother & clone on week 4). I have had to change things up a bit. The test with/out Green Sensation is still going forward. But, I'll be using Xpert Nutrients (for both mother & clone). Momma will go on Green Sensation after pre flower. The clone will be flipped on week 6-7. I won't make the contest. But, it'll still be a good test that I'm determined to finish. Giving results to show the clear difference with/out Green Sensation. I've started the mother on Master Bloom from Xpert and Co. The clone has started a light Master Grow Feed (200ppm) with Master root. Mother 70 days old Clone: 10-12 days old. Both are thriving. The mother got a little ph issues during the flip process but is fully undercontrol & has provided a good base for the test to continue without risk tainting the end results. Clone will be transferred to a 9L pot. And fed more or less exactly as the mother. (I've also got a spare clone) I'll be keeping in the veg state for the summer. As if I get really nice results from this pheno. I really want to make sure I have it again & again. Runtz been one if my favourite strains. DISCOUNT CODES 20% OFF ZAMNESIA WITH CODE: ZAMMIGROW2024 20% OFF XPERT NUTRIENTS/KANNABIA with code: GGST 20% OFF ORINGAL SENSIBLE SEEDS WITH CODE:

Only flower fuel iv upped intake from 1 TBS to 3tbs She is blooming nicely she has a smell like no other i think my clothes smell like it

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""