2/17/2024 - Vegetation Week 1 Day 1- I removed some of the water to see where the roots were only one had roots in the water. However I want to go ahead and get them on Week one Nutes So I am over 50 gallons of water in the system so I mixed the Trio at a 1.0mil per Gallon but restricted it to a top of 50 gallons period. I top fed the other two to keep encouraging root growth. I also finished my drying and curing from my last run and wanted to show what it looks like all bagged up and in the cannatrol. I added the following Nutes: Silica = .5Mil/Gal= 25Mil CalMag= .75Mil/Gal= 37.5Mil FloraMicro = 1Mil/Gal= 50Mil FloraGro= 1Mil/Gal= 50Mil FLoraBloom= 1Mil/Gal= 50Mil ORCA = .5Mil/Gal= 25Mil EPSOM- 1 big pinch 2/18/2024 - Vegetation Week 1 Day 2- I top fed #1 and #3 since they still don't have roots in the water, I will continue one top feed a day until roots hit water. 2/19/2024 - Vegetation Week 1 Day 3- #3 now has root in the water.. Yay!! I now have #2 and #3 with roots in the water so no top feed for them. I only had to top feed #1 and the only reason I didn't just go ahead and cull her today was I am responsible for breaking her tap root and stunting her, I wasn't careful enough and she was the first to show a good root. In that vain I will carry her a few more day's top feeding but if not root by the end of the week she is getting culled. 2/20/2024 - Vegetation Week 1 Day 4- Today I Ensured the PH is in range, I refilled the Humidifiers, I top fed #1 since the roots are still not in the water. I also removed the humidity shields from #2 and #3 today the ladies were touching the sides so they are done with the extra shields.. 2/21/2024 - Vegetation Week 1 Day 5- Today Morning: I Ensured the PH is in range, I refilled the Humidifiers, I top fed #1 since the roots are still not in the water, Evening: I decided that the roots of the two are looking great so I am going to drain and bump the Nutes to a full Week 1/ Week 2 Nute base. I added 36 Gallons I added the following Nutes: Silica = .5Mil/Gal= 18Mil CalMag= .75Mil/Gal= 27Mil FloraMicro = 3Mil/Gal= 108Mil FloraGro= 2Mil/Gal= 72Mil FLoraBloom= 2Mil/Gal= 72Mil ORCA = .5Mil/Gal= 18Mil 2/22/2024 - Vegetation Week 1 Day 6- Today I Ensured the PH was not too high as the system continues to cycle the water and adjust, I refilled the Humidifiers, I top fed #1 since the roots are still not in the water, 2/23/2024 - Vegetation Week 1 Day 7- Still have to Top Feed #1 no roots in the water, however she is still progressing nicely slower than her sisters with roots in the water but still going strong.

Such an amazing smell of this lady and its only going to get better. Nice stacking going on and the colas appear fatter than the other strains in the tent. Nice and frosty too. She got little to no training, instead she was topped twice. The smell though! Droool!

sorry for this week of delay, I didn't have internet modem broke in the week of the photos, but we're back and moving on with this beautiful girl, increasingly fat and fragrant something like high color cake and very compact buttons as usual at Hydro I look forward to this coming soon!😉

Day 106 14/10/24 Monday Their colours are really coming through now 🤩 Nearing their end now, possibly another week after this ✌️💚 Picture and video update 📸💚 Day 108 16/10/24 Wednesday Another run through of de-chlorinated tap water and flawless finish all to pH 6.3. Giving 0.3L again daily as there not drinking half as much now. Picture and video update 📸💚 Day 110 18/10/24 Friday Divine Seeds Overdose has been Harvested. Check her own diary on my page for full results. I moved the others into my now cleaned down 1.2m x 80cm under 660w hps to finish off. Day 111 19/10/24 Saturday De-chlorinated tap water and flawless finish at pH 6 today.300ml Day 112 20/10/24 Sunday De-chlorinated tap water and flawless finish at pH 6 again 300ml. I have had an extremely busy week. I'll upload videos and pictures tomorrow on a fresh week... Wait and see the colours 😋💚

Toujours en rinçages. quelques trichomes bruns sur une tete matures, mais sur des tetes plus basse, on a encore des trichomes transparents, je pense qu'on arrivera à terme la semaine pro !

Wow the smell in this tent hits you in the face like a sack of lemons if life gives you these kind of lemons you take em and smoke em 🍋🎄🤤

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

She is getting the final bulk now and soon i will shop her down sbout 5 days left :D

topping to lollipoping

Es realmente impresionante ver el crecimiento que ha tenido en menos de 1 mes. Unos días más de vegetación… quizás sale alguna poda apical más, unos días y luego a florar 🌹😍👍💪🦍🦍🦍

D-70 : At this stage, I'm a bit.... surprised that the Desfrans are not taller thant they are. The smallest one is 35cm wich is really small for a 100% sativa strain. The tallest one is about 56cm, wich is also quiet small I think. If I compare with some other diaries, the tallest one could be in the target if we take into account that I trained them a lot during their first weeks. But the small one is almost the half she should be... There's no sign of deficency, none ! I have 420w @ 50 cm from the top, so it's not because of the light. The container is a 20L smartpot. I simply do not understand. Is it juste because of that phenotype ? Will she grow during the next 7-9 weeks untill she'll recover her late ? I Modified a bit the position of the light so the Lumatek 100w FullSpectrum on the side of the box is really pointed to the "slow" girls. We'll see if it change anything but I really doubt about it... The only lead I have is : Desfran loves HEAT and Light. The 2 smallest are on the coldest side of the box, even if it's not cold at all. But it is the side near the window...

Tied her down and regular defol as the bud arms start to form shes real bushy and looking like she’s about to stretch out I wouldn’t be surprised to see her at around 70 cm this time next week . The tent is getting mad full

After another weekend spent away from home, on day 26 both Opiums present at 30 cm height, having grown big and bushy. Their thirst increased, I will water them when their next day starts. And do some more LST. Not too long until I can install the scrogging net. I am really impressed by their progress. They developed a lot of branching side branches and look really healthy and bushy. In contrast to what I wrote before, I think I can stay a few days longer with the current 80% 18/6 lighting scheme. Leaf positions do not show signs of light hunger, so no reason to change. Watered them with 1.5 l each on their 27th morning, this time adding some flower fertilisers too. Compensated their relief with some more LST. I can see in timelapse video they are tmelselves compensating this stress easily; it’s amazing to see how fast they turn their heads. You’ll see soon too. For reference: Pot diameter is approx. 25 cm. So lady #1 spreads about this much, lady #2 is even about 10 cm more. Guess they’ll make a very dense scrog surface, but first let’s have them have their stretch. Day 28 evening revealed ladies are getting thirstier. The holey pots might be helpful in root development, but they are not when it comes to keeping the tent clean. Because of their structure, water runs out in respectable quantities each time they are fed, which takes a long time to evaporate and adds to air humidity of course. Which on the other hand is currently quite ok. Outside air temperatures are around 30 °C the last days, so a bit more humidity helps to keep the VPD in range. Which is quite ok as I still cannot complain about their growth – around 2–3 cm each day currently. Grow video for day 28 (sorry about the interferences! Due to the control hard- & software being offline I cannot silence the blower(s) when a photo is taken anymore) shows steady growth and very vivid movements again. Dropping of their leaves around their evening is possibly due to me watering them again as soil was surprisingly dry already. On their day 29 evening, it’s clear to see how much they are stretching: They really gained 5 cm since yesterday! Lights are close to 30 cm, so I think I’ll reposition things a bit when their next week starts. Attached video shows the day’s progress. They say you cannot scrog an Automatic but seeing the development speed and bushiness I decided to give it a try nonetheless. I guess the buds can profit from being a bit more apart from each other if I handle to fiddle them through the holes in time. I rearranged the lights to be at about 45 cm apart from the tops and set the lights to 100% at a 16/8 lighting scheme, waking them up one hour later than before and sending them to sleep one hour earlier. Last day of this week, day 30, shows that growth speed has even increased. Lady 1 crosses the scrog net already which is positioned at 46 cm above soil. Gave them slightly enhanced water again, 2 l each.

Purple Chill aka Penny was easy to grow. She was medium height 26 inches. Stem structure was weak. Many stems bent (repaired with splints and masking tape) and some broke off. She suffered from heat stress and also showed cal-mag deficiency.

Starting off week 8 pretty solid. High Tide amendment from Commonwealth Canna has really helped the plants out. Added a 4 foot stake to the tallest plant. Pound Cake does not need much nutrients. My tips were burned as a result of too much Humic Acid in weeks 3-4. You and learn. Besides for that issue, they are rolling alone very nicely.

I'm going to try and get some better angles. My screen is smashed on my phone and shows different colours. First week of flower, let's see what Barry does with what he's been given eh?

Alla fine sono uscite 4 su 5 piante Una Future Auto#1 non c'è là fatta Continuo a farle crescere senza stressarle, visto che al momento sto usando una 300 watt Lumatek, anziché la mia Marsh Idro 480 watt "Evo".. tutta un altra storia per 4 piante, anche se la Lumatek è un ottima luce...ma con 180 watt di meno! Metto se riesco degli aggiornamenti con foto giornaliere o 3/4 volte a settimana, così da vedere gli sviluppi in tempo reale 🙏

This week I transplanted my ladies to their 3 gallon oxypot. They both look very healthy with a big root mass and strong stalk. Topping in 4 days or until they are settled in proper.