Breeding work.

1-1-22 moved male into tent with mother filpped to 12/12. did more work on the 4 female f1s during defoliation and moving of ties one of the main branchs snaped, its the frontmost right plant, poorly used tape around stem but short internodal length and snap sight made it difficult 1/4 of stem attached taped leaves to other leaves on good branches as a low impact way of closing snap. watered today just by habit of judgment no measuring as each style of phyno is drinking different rates. 1-2-22 male opened pollen as anticipated did first round of pollination today, tagged 2 locations of chosen female f1 to make f2 seeds (small number only) and mother ADF was given the shake & blow pollination method with her son ADF x SS f1. didn't take extreme precautions as have done in past, f1s were not washed down after as are in separate tents but both were open next to one another, no further preflower type herming was observed on f1 females, any seeds found will be saved as f2 of male cross, as currently f2 mother is chossen on looks alone. 1-3-22 second round of shake and blow pollination for Bc1 seed ADF x (Adf x Ss) male. visible pollen in air observed, didn't really float long with the vetilation on and no fans it mainly went up to the filter, today second tent with f1s was closed, washed hands and arms water only and took off robe before checking on f1 females; defoliation on a small scale has been on going. curious to see how lax i can be in this set up as far as pollination. also switched back to 18/6 for Mother ADF to keep cuttings in veg, realized it takes a while to re veg and I'll be done with pollen hopefully tomorrow after collection possibly. with only negative pressure on both tents, better to learn now then later f2 seeds are never dull. will follow tomatoe seed line examples with a number keeping track of each individual and grow each line in parallel keeping track of each individuals ancestor to be able to weed out negative traits. exm. adf x ss f1 plants 1, 2, 3, 4, pollinated by only male. 1-1 1-2 1-3 1-4 1-5 ect. each generation a chance for a bulid up of negative traits may occur, and be expressed, but depending on frequency lines can be eliminated, say after 4 generations plant 1-3-8-2 has seeds 1-3-8-2 -1, 1-3-8-2 -2 exc and none of her children say 100 of them are expressing your triat and same is true for say the 2 line, but the others lines 3 and 4 have a 50/50 ratio of trait, then you'll know the genetic phenotypes and bonus know whitch males of whitch inbred line are worth keeping. thats true for each generation 1-3-6 may only have high thc offspring for another 7 generations and be stable for it same with every seed of 1-3-6s family including the males. but say the 1-3-8 line after another 3 generations you still have low thc, that line can be retired from this project but possibly kept if it has say very strong mould resistants or a locked in color, terp. may come in handy. pipe dream if (or how many seeds) i get for being lazy. but its exciting to think id be just as happy with seeds rather than bud this grow. and we'd go back to known pollination procedures with the bags and no ventilation and spraying water on the gals for bud. fingers crossed the second tent will allow me to see if lazy works with this set up. 1-4-22 well lazy seems to be working good enough for me, first two hand pollinated sites on f2 seed mother are darkening pistels and drying out without the whole tent doing the same. on the down side a few pistels on the 2 more indica looking plants are darkening also, they are closest to the vent that is next to the second tent, pollinated likely by pollen dirfting down and being sucked through those 2 plants. seeing as 3 of 4 are going to give me a few to decent number of seeds I went ahead and chose a seed site on the middle f1 plant to give some seed as well, she was hand pollinated today. ADF was pollinated again by ADF x SS male with shake and blow but under max negative pressure today. pics of darkening pistels are hard to see but in person it is quite obvious. 1-5-22 Saved ADF x SS f1 pollen in paper, in a jar glass suspended above some calcium chloride desiccant. will store in fridge tomorrow for a day then freezer. took male down but saved a cutting in a glass of water to have a few more days of fresh pollen if needed. did final shake and blow and hand pollinated most of mother ADF for the last time. gave some epsom salt solution to all the plants. 1-6-22 potted up margelo cuttings into gallon accelerater pots, mixed old male dirt in with new soil worm castings and bone meal, feel like shit surgery tomorrow, didn't measure. watered with week super hume and seaweed, molasses soultion. mother ADF is showing N toxicty and Mg lockout. she was watered two days ago, and so yesterday she got Mg solution as already wet, watering done with rain water, will continue rain water for rest of grow its slightly acidic and should help leach N faster. F1 females watered with well water, HST possible herm plant inspected no signs of male flowers or many dark pollinated pistels, all plants were inspected and no herming was observed, pollination sites checked and with desire for more f2 seed Pollen from male cutting in water was re applied to seed sites and new random seed sites selected non of 4 main colas were selected as seed sites as i want as good a reading on each potential mother as possible. 1-8-22 seeds on mother ADF will be BC 1 ADF x (ADF x SS) , F1 gals/mothers look good f2 seed set in small numbers. no watering. saved pollen f1 ADF x SS. in paper and jarred with other pollen.