Day 13 is when we saw very healthy and explosive growth. I turned on my air pump on day 14 and filled + turned on the reservoir on day 16. It's crucial that you blow room temp. air into the root zone to promote growth. The roots are well established around 2nd week and that is when you should turn on your AutoPot so that the plant learns to uptake water and nutrients from the bottom of the pot rather than from top-bottom feed.