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2025-09-26 We are under 13 Hours of Light, we had a cold and rainy Week from Sunday on until today ( Friday) weather was cold. it was raining heavyly- and iam hoping some dryer and warmer days to come for the moment everything is fine, plants are standing under the Roof and getting stabilized by 2 SCROG- nets we will see.

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This plant looks sooo cool!! I´m goin 2 top her again only on 1 side. Try a cewl shape 😎😎😎 2nd time topped, only on 1 side. I´m goin 4 a scorpion look 😉 she looks wir war

Week 6 of flower I think we have an issue. Haven’t noticed any bulking in the flowers in the last two weeks. The gcc has slightly but the Saturn hasn’t at all. The Saturn is staring to fade into a light green yellow witch is normal. Also they are not drinking as much water as they usually do where last time I ran them that did t start till week 8.all they got this week was recharge one day and silica on the other.But we will see I have about 3 more week I think. Probably give them one last terp tea bloom tea

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Hi everybody this week went okay I noticed she looks a bit hungry so I've risen the PPM to 800 . Ph at6.8 . I had done a second lot of defoliation to enable light to the bud sites. She smells amazing has a very complex terpene profile when I smell her she smells like cookie dough on my fingers then after a few seconds it starts to smell like lemon sherbert then after that I can smell pink grapefruit . It's amazing it's another reason why I germinated another gorilla cookies but in coco. Well that's it for this week thank you for looking at my diary will be update every week. And always keep in mind it's 420 somewhere 👑🌱

Hello beautiful people! Another week of veg! The girls are doing great! I topped them once. I pulled them down for some LST, will continue to pull down most new shoots. Nute schedule still the same. Its already clear this is a heavy sativa strain. Thats it! Love & Peace 4 all!

During a beautiful spring evening somewhere in May, the idea came to me to choose for a new hobby. And because I enjoy gardening very much and also like to smoke a joint, I decided that night to try to grow my own weed. And I also wanted to challenge myself to grow the best weed I possibly can. That's when I started to read up on all the facets of growing cannabis. From materials to technique, from strains to the anatomy of the plant, I prepared myself for at least 2 months before even one seed went into the soil. And partly because of this thorough preparation, I can now say to myself afterwards that this is the maximum I managed to get out of it. And the great thing about all this is that after these past 4 months I have learned so much more! Therefore I already know for sure that my next grow will be even better. Below some tips for my fellow newbies, they might help you during your own grow... Material In addition to the lamp, the tent and the right nutrients, which I commented on above, I have benefited a lot from a humidifier and dehumidifier over the past 4 months. Especially in the first weeks of growth, a humidifier is very useful so that you can keep the humidity around 60% (or what the VPD chart tells you depending on the temperature). During the flowering phase it is very useful to use a dehumidifier so that the humidity remains below 50% and at the end of the growth even close to 40%. That way you also exclude the risk of mold and bud rot. It is also useful to use these devices during the drying process so that you can keep the humidity around 60% and dry your weed slowly (at a temperature of around 15.5 Celsius, drying takes about 14 days). Drying slowly and coolly definitely benefits the smell and taste! What's also really a must are an EC and pH meter, without these 2 instruments it is actually not possible to realize a perfect grow. Make sure you use a reputable brand like Apera, they are a bit more expensive but always deliver spot on. In total I invested about 1500 euros in material on my first grow. This is also because I wanted to do it perfectly and therefore only went for quality products. But all in all I think I earn back my investment within 2 runs, taking into account that both my friends and I will never run out of a joint 😊 Seeds Good genes are important to ultimately achieve a good end result. Therefore, do not save on the seeds and buy them from a reputable breeder. That way you can be sure that you will grow strong and healthy plants that can withstand pests and other negative influences. Well begun is half done and this is especially true when growing cannabis. Technique Of all the training techniques I have used, I would like to specifically mention one that surprised me the most: Supercropping! Because I did this grow using the ScrOG method, I wanted to create as even a canopy as possible. The type of lamp I use is also most suitable for this method. But no matter how well you train your plants, they will eventually stretch unevenly during the first weeks of flower. I therefore snapped about 8 stems using Supercropping, which made my canopy nice and even again in a way that each branch gets the same amount of light. And the colas on those branches turned out SUPER nice! Also the nuggets beneath were growing bigger because they too got more light. I will certainly use this technique even more in my next grow! Will I will definitely use a diary again I will definitely use VPD charts again I will definitely use a feeding schedule again I will definitely turn my light on during the day and off during nighttime again I will definitely use the ScrOG method again I will definitely choose a feminized American strain again I will definitely use a webcam again (Wyze Cam) I will definitely defoliate even more during the 4th week of flowering I will definitely go higher on lollypopping I will definitely Supercrop more I will definitely dry my plants slowly and cool again (15 days at 60% RH and 15,5 C) I will definitely continue to learn more and prepare myself even better for my next grow again 👊 Won't I will certainly not water my seedlings too much again 😅 I will certainly not go for 3 plants again, but instead for 4 plants per m2 I will certainly not hesitate to ask experienced growmies for help again 🙏 (next week I'll comment on the bud dry weight and give my final smoke experience)

Really late to post this week so gotta do next week aswell tonight, plant been doing really well, quite different to the others in terms of height, much higher and plant is almost touching lights, regardless she has been doing fine no problems, she seems to be able to really hack the heat as she’s not even burnt at top, fattening up a bit more now. Overall quite pleased

The temperature is high in the box, 30 degrees is mistaken unfortunately. I think it looks pretty good at this temperature, and it grows well .. What do you recommend? I am a beginner.🙂💚

These girls were in the back of the tent, and probably received less love and the girls upfront. I diddnt have any issues with them. Of the two plants I got 140 grams dried and trimmed. She definitely need a cure right now I’m not getting any smell. Even through out the whole grow the other plants over powered her. I wasn’t a big fan of this pheno. I’m definitely gonna pop some more beans try to find one with more terps. But the smoke is very potent. I smoked some once dried and this shit put me on my ass. I’ll update in a month after the cure In the grove bags. She had a 15 day dry

Day 64: Started flushing the girls today. Got All milky and starting to go amber. Day 65: Still flushing. The girls smell amazing. I have one in the bunch that is SUPER airy and I am just not sure what is going on with her. It's the girl that I accidently broke off the main cola a while back. Not sure if that has anything to do with it or not. Day 66: No new updates. Cut one of the colas off for a taste test and trial run on drying as this is my first go. Figure if I can dry this super dense beauty with no issue, I will be able to dry the rest. Have it hung up in the spare bathroom. Stays roughly 71° in our house and I'd say roughly 40%ish humidity. Day 67: Girls are smelling really nice! I keep looking at trich's and looks like no change. They are all cloudy and only a few amber from what I see. Still new to it so no tellin! Day 68: No updates. Day 69: Girls still looking good. My bud is still drying and looking super hideous. I feel it is drying slow enough but sure doesn't look or feel dense as it did when cut. Will know in several days to come when fully dry. Day 70: Girls smell dank and look the same! No new updates at this time.

Day 28/06/2024 I chopped the first one, day 08/07/2024 I chopped the second one and today day 09/07/2024 I'm going to chop the other two (bigger ones) Very satisfied with all this, just the beginning and you will also see more diaries around here! These plants were perfect for a newbie like me, but I also learned that I'm very good at this, all the knowledge were in my head. See yall! 😎👻👽😈 Edit: great smoking, relaxed but not much couch lock, good after a day of work, will try on days of to see, I’m fucking happy bros!!!!!

+ Day 57 : Pantime Day 55 : 4L mixed Water (EC 0.577) -5.8PH-20ml-Flores +

Semana de entutorado y defoliación 👨‍🌾🏻

I have at least 3 weeks to go on this Green Poison, hoping the Ohio weather holds out for me ✌️🌱✌️

Full flowering has officially commenced. Lots of bud sites, a bit more defoliation and opening up. I untied the middle four colas so they can shoot up. I’ll be adding a second layer of lights for the lower canopy in a week or two.