We got the first purple strain ❤️❤️ Its amazing how purple the buds get, the banana purple punch is a very easy strain to grow. I mean look how big she get, realy big 😂

Marker didn't take well to the mainlining process; as a result, one cola was almost entirely consumed by mold. However, overall, things are looking good. I ended up with a small, resilient plant with a nice aroma of pineapple and Skittles, with hints of gas towards the end. 🍍🌿 Towards drying and curing now! 🌬️🎊

I will be growing her again, as I feel like I let her down, by letting her PH drift so low.. Other than that, she grew beautifully, and fumes of potency as you gaze at her! She yielded more than I expected! She's not that far below my previously lowest yielding plant! :)

On the 29th, irrigation was not possible for 2 days (25% dry leaves), they probably got a shock... Electricity fell into it. We'll see what happens in the end. Currently 33 days in bloom.

Apple Betty Seedlings 🌱 are doing great! They are looking very healthy and happy. #1 was slow from the get-go but she has been building steam and is doing well. Routine this week was was just checking the humidifier every morning, and spritzing with my spray bottle x2 daily (very small amounts) starting at the base of the plant, but also making sure to get the exterior edges to allow those roots to be searching for water. Changed the light cycle to 18/6 around day 7, and also adjusted my BloomPlus BP 2500 to have 140 watts at the wall. Didn’t lower the light as the increased wattage seemed to slow down the stretch they initially had. Still using my small 250 watt heater behind my intake fan to draw some heat in and keep the tent nice and cozy, even during lights out (about 75-76 *F) By day 14, I started to water with a measuring cup, still using my 1 ml of Sensi Cal/Mag extra. Watering only about 1/4 cup of water all around the pot, and will wait until the soil is dry’ish (the old finger poke) before I start to increase the watering amount gradually. No nutrients yet, but I will be using the Advanced Nutrients- Grow, Micro and Bloom along with the Sensi Cal Mag extra. Happy Gardening 🇨🇦❤️🌱😎💨

Things progressing steadily in the tent. Smells us developing daily really stinky in there at the moment. The Apple kicking out a really pungent chem smell. The banana giving off a sharp citrus smell and the cherry a funky fruity smell.😍😍😍

We loved this photoperiod Super Boof from Zamnesia right from the start. This plant has developed an excellent cannabis body that has allowed it to give us beautiful flowers, with good density, a great scent, and excellent resin production, especially considering the hot periods it experienced. Up until now, I haven't had the chance to control the temperatures with air conditioning, but I'll soon be able to do so at the new location, and everything will be even more precise. However, finding an ideal spot and making as few adjustments as possible remains the ideal solution. I love hillside locations; at most, you need to cool them a little in the summer and heat them a little in the winter, for sure. In any case, we're gearing up for full control; I'm still a home grower like everyone else. Our plant has given us good trichome maturation, mostly milky/cloudy, just as I like it, with some amber and still a bit of clear stuff. Always cultivated from start to finish with Plagron fertilizers in the 100% organic version, soil included. Leafy's Strain of the Year ---- // https://www.zamnesia.io/en/13317-zamnesia-super-boof-seeds.html Zamnesia Quick Facts // Here's your chance to grow one of the most sought-after strains of recent years: Super Boof, rightfully crowned Leafy's Strain of the Year 2024. Derived from Black Cherry Punch and Tropicana Cookies, this 70% indica-dominant hybrid takes the already exceptional traits of its predecessors to new heights. Grow it at home to find out for yourself. The whole world of growing and more is at Zamnesia - just take a look around the site and you'll find "nature's best" in all shapes and colors. New strains are awesome and old ones are no exception... -- // www.zamnesia.com

Keep on growing babies!! Lest get it Scandinavia!! A bit of nutrience, a joint n wee ouuut!!

Nichts besonderes! Die Seeds sind gekeimt wie geplant, wurden dann sofort in Steinwolle gesetzt. Nach 3 Tagen in der Steinwolle waren alle Ladies am Tageslicht. Nach nur 2 Tagen in der Steinwolle direkt in coco töpfe 1 l eingesetzt. Klima ist nicht ganz perfekt, bekomme es aber nicht besser für diese Phase hin. Ventilator läuft 24/7, besonders für die kritische Zeit ohne Wärme der HID Lampe arbeite ich in den Wochen 1-5 mit CO2 Bags, da ich wegen dem Klima (draußen 0 Grad) keine direkte Zuluft nutzen kann.

Hello guys, I fixed my RH problems. I now water them 1,5L every 2-3 days, the Shimos seems to still be thristy ! I did a light defoliation, then flipped into 12/12 after 32 days of vegetative stage. This will be interesting 😍 Have a nice day, see ya next week !

Its day 25 since sproud and the plants are looking good so far. The jslow stress training with the clips works as expected and the branches are getting thicker and more robust. Defoliation on day 34. I collected leave mold from the surrounding woods. a lot of white fungy hopefully usefull mycoriza.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

She throwing off some Phat buds!

Day 85 - Starting week 13 today and still no issues. Checked some trichs today and still quite a bit clear in there but quite a bit cloudy. Going to start flushing her for her next watering and looking at her, think she’s got about 2ish weeks left. Day 86 - All still on track for a chop in about two weeks. There’s less and less clear trichs but would like to get a good amount of amber before cutting so hopefully she’ll push them out in the next two weeks. Day 87 - Gave her 2 gallons of plain 6.5 pH’d water today to start flushing out the nutes. She’s almost all ccloudy now and hopefully start getting some amber in there in the next two weeks before chopping. Day 88 - All is going good still. Checked the trichs all over and it’s pretty much all milky and seeing a couple ambers in there. So it seems like about a week to two weeks left. Day 89 - No real update, she’s getting a bit more amber in her so almost thinking she’ll be ready to chop this weekend. Just gonna keep an eye on her and when the trichs are right, she’ll get the axe. Day 90 - Lots more amber are showing now so looks like it’ll be day 91 or 92 when I chop her. Will probably chop her on Friday but may end up doing it tomorrow. Not sure yet, depends on how I’m feeling and what she’s looking like tomorrow but definitely excited about getting her chopped, trimmed and start drying her. Day 91 - The end of week 13 and Letting the girl finish drying out today and will be chopping her tomorrow. She has some lower fluff bud but everything else is ready.

I am not worthy! I am not worthy. I must start out by saying that I should never have doubted this girl when I first started growing her. The outcome ended in 2.7 oz. And .5oz of trim which i left in my shed and lost to mold. :/ First hit is like getting brain freeze! Its that strong. I have never grown anything like this before. It is denser, stickier, smellier and stronier than anything I have ever grown! There is so much resin. I cant wait to she how she does as an oil. I plan on continuing to grow the rest of the 6 shooter seeds I have until I run out! Taste is of a heavy diesel, slightly woody with some sweetness to it. Very smooth too. The high lasts for hours! It starts off at about 75% strength and then peaks about 20 min later. Has you walking all over the place picking shit up and setting down in random places. Constsantly on the move with this one. After about a few hours, the relaxation and pain relief set in. I mix this with some of my cbd buds and it works wonders. That you so much fast buds! Excellent quality!!

..week 8 of BlackOpium.. and she is still flowering strong💪 she has a weak,peppery smell.. love it..reminds me of the 90's..dont know why..mayb i am just being nostalgic (in 90's i smoked my first...😎👊) She has been in safehouse all week bc of wet and cold weather but thats no problem 4 her.. she is tough!💪 Now some sunny days have arrived 🙌 so she can stand in garden again👍 ..am waiting for the sun to hit her..😸 so i can make some nice pix...😍 (i hope..am having a bad'days"..its hard for me to get it nice and sharp, my hands are shaking..*MS* and have no energy😒.. doesnt matter, am used 2 it..i will get it done,no matter what!✊) Happy growing 4 all✊ **KISS! growingtechnique: keepItSimple, Stupid! PS i think i may have 'jinxed 'it.. it was almost impossible to make a nice pic..nothing was sharp.. so i just made a small video clip😉

This plant drinks like a camel

Will post dry weights & more comments soon! Sorry for the lack of updates but Thanks for following & happy growing friends!✌️🏼🙏🏼🌱