Day 80 for the 54 plant grow. 42 different strains of photoperiods and 12 different autoflower strains in the: 8X8 GROW TENT WITH THE NEW          FC-E6500, FC4800 AND TSW2000 and green buzz all organic/vegan fertilizers🤘. I only pinched the tops on 2 or 3 plants but I defoliated every plant ALOT. it slowed the vertical growth alot but it's exactly what I wanted. I can keep track of the plant sizes, how they grew, how healthy they were, how easy they were to grow and how good the final product is👍 The girls are loving their MARS MANSION and everything is doing good and looking nice and getting bigger👍 lots of hairs clumping up now and they are all getting nice and frosty and starting to stink ALOT. MARS HYDRO ALWAYS GETS EM FROSTY👍.I AM NEVER DISAPPOINTED With how awesome these lights are🤘. KEEP KILLING IT MARS🤘👏👏 BREEDERS AND SEEDS: DIVINE SEEDS: MAZAR, OPIUM, WHITE WIDOW, OG KUSH, PABLO ESCOBAR, DIVINE INDICA. AUTO WHITE WIDOW AUTO PABLO ESCOBAR AUTO WHITE RUSSIAN AUTO FRACTAL AUTO MONTREAL AUTO OPIUM AUTO KABUL AUTO MAZAR. WEEDSEED EXPRESS: GREEN CRACK, GHOST TRAIN HAZE, LITHIUM OG, CHEESE. DOCTORS CHOICE: DC KUSH, AUTO NIAGARA, AUTO ROCK MACHINE. EXOTIC SEEDS: HIPPIE BERRY, MALASANA GELATO, EXOTIC ANIMAL. GENE/MEME SEEDS: BLACK HELL. ORIGINAL SENSIBLE SEEDS: PURPLE PUNCH COOKIES, WEDDING CAKE, PURE KUSH, GORILLA COOKIES. SWEET SEEDS: BLACK JACK, GORILLA GIRL, SWEET ZKITTLES. EMERALD TRIANGLE SEEDS: GORILLA GLUE, MASTODON KUSH. SEEDSMAN SEEDS: BLACK SUGAR, PEYOTE FORUM, PEYOTE WIFI, BADAZZ COOKIES OG, PURPLE GHOST CANDY, STRAWBERRY BANANA GRAPE, L.A. PEYOTE KUSH, PEYOTE WIFI CBD, FUEL DOG, CRITICAL PURPLE KUSH. AMSTERDAM MARIJUANA SEEDS: GIRL SCOUT COOKIES EXTREME, GREEN CRACK. QUEBEC CANNABIS SEEDS: BRUCE BANNER, GREEN CRACK, QUEBEC CBD 20:1. I 💚 GROWING MARIJUANA: SKYWALKER OG, BANANA KUSH, BLUEBERRY CBD. FOR EVERYTHING MARS HYDRO VISIT: www.mars-hydro.com www.marshydroled.ca PROMO CODE: rocknroll VISIT THE ALIEXPRESS STORE: @marshydro_aliexpress Instagram: @marshydropenny https://youtube.com/channel/UChU5ZWwNi-DvnO3oWVU3ceA https://greenbuzzliquids.com/ #greenbuzzliquids @GreenBuzzLiquids https://greenbuzzliquids.com/en/shop/ PROMO CODE: rocknroll https://www.youtube.com/watch?v=8hEqyS1jPpI https://growdiaries.com/diaries/112437-grow-journal-by-rock-n-roll-randy/week/593118 Thanks for taking the time to read and check out my diary🤜🤛 FC-E6500 LED GROW LIGHT: FC-E6500 grow light with 3978pcs OSRAM BridgeLux Diodes & Moso Driver, market-leading PPF 2.7 µmol/J, Max 2.5g yield/watt with 30% higher Average PPE, more cost-effective when compared to same style type lights. Only consumes 650W, perfectly cover 5x5ft (personal), 4x4ft (commercial) grow space. Innovative Precise Uniform Lighting-Built-in removable bars interval & dimmability enable the exact & dynamic PPFD control over each of the plant growing stages, FC-E6500 detachable bars deliverSuperior Heat Dissipation: Real Aluminum heatsink together with 6 slim profile passive cooled bars (THINNER & LARGER surface) and detachable Meanwell Driver, create less heat. save 50% hydro than other old grow lights! Driver can be mounted remotely on the side of a grow tent/rack or on an adjacent wall to remove heat from grow space. Full Spectrum UV IR LED: With Yield Max spectrum (660nm Osram enhanced Deep Red,2800k-5000k,730-740nm IR,380-410nm UV LEDs), Average PPFD the FC-E6500 LED grow lamp enables cultivators to produce BIG FAT Dense Buds, perfect for full-cycle hydroponic, soil, grow tent/grow room. Dimmable Commercial Grow Light: Cost-efficient, easy dimming & daisy chain With a max up to 20 lights, IP65 Waterproof, master light easily controls the rest. Perfect for commercial & home horticulture. FC4800 LED GROW LIGHT: GET THE MAX FROM MARS👍: Welcome one of  the newest members of the Mars Hydro family🤜🤛🤘. The Mars Hydro FC 4800 480W LED Grow Light can accommodate up to a 4' x 4' area for flowering. It has OVER 2000 LED chips consisting of the best selling Samsung LM301B diodes which has a Luminous Efficacy of: 220 lm/W 0.2 W, 3 V mid power, Osram 660nm Diodes, Infrared light and UV LIGHT👍. It boasts the incredible efficiency of 2.8 µmol/J, maximum 2.5gram yield per watt with 30% higher Average PPFD. This High-intensity spectrum is beneficial for plants at all stages - from veg to bloom, and creates Big dense Buds👍. achieve 50% higher yields & quality compared with the old lights! Only consumes 480W for 4x4ft personal grows, and 3x3ft for commerical grow. It comes with a dimmer and daisy chain function with a  maximum of up to 15 lights, the master light controls the rest👍. Wide 6-bar design offers even & wide canopy coverage and can maximizes your grow space. slim 180° FOLDABLE DESIGN... YES I SAID FOLDABLE🤘🤣 , making it compact and easy to install, it's IP65 WATERPROOF and will give you no worries while growing in a humid environment and makes it a flexible high performance commercial grow light for enormous  SUPER FROSTY yields.😛😛😛. MARS GETS EM FROSTY BOYS👍 AWESOME HEAT DISSIPATION: The Low energy consumption FC4800 growing light has 6 passive-cooled bars and an aluminum heat-sink a removable driver  you can upgrade later or have it outside your tent. with zero noise  you can save up to 50% in your electricity bill compared to HID's or HPS lights! It also has a removable driver so you can upgrade it in the future or have it outside your grow space for better heat control in your growroom👍 UPGRADED FULL SPECTRUM: Yield Max Spectrum with enhanced red (Top-Bin Samsung 3000k-5000knm, Osram 660nm Deep Red), speed up blooming & fruiting, perfectly suited for full-cycle growing. The FC4800 LED grow lamp was born for Big Buds, perfect for commercial, grow tent, grow room, hydroponics, and all soil grows.👍 THE BEST SAMSUNG DIODES: THE FC4800 has Top Bin genuine Samsung Osram Diodes, combined with remarkable 2.8 µmol/J Efficacy, 30% higher Average PPFD, 50% less energy consumption, to maximize your yields. They give you a TRUSTED 3 years warranty 100% Satisfactory guaranteed!!! TSW2000 LED GROW LIGHT: INCREASE YIELD AND CROP QUALITY WITH MARS HYDRO LED GROW LIGHTS: NEWEST SMD LED technology provides the highest PAR/LUMEN output (1122umol@18") and can make you get 30% higher yield compared to OLD blurple led lights.  Max 2.5g yield per wattage and achieve maximum quality and quantity, much better than traditional HPS grow systems LOW ENERGY CONSUMPTION LED GROW LIGHT: Consuming only 300W true output with 684 LEDS! It saves up to 50% energy than other led grow lights. Perfect for 3.5x3.5ft bloom stage, 4x4ft veg stage. Over 90% light energy can be absorbed by plants, it will have higher intensity in a MARS HYDRO grow tent with diamond design mylar, or by crossing over using multiple TSW2000 HIGH EFFICIENCY FULL SPECTRUM INDOOR GROW: 3000K 5000K and IR 660nm 760nm RED, makes it close to natural light, highly engineered to provide improved growing performance for plants at all stages of growing, rapid plant response from seed to flower; Uniform and wide-beam light distribution for your plant's optimal growth 8x8 GROW TENT: The MARS HYDRO 8x8 Grow Tent interior is made of a unique diamond design mylar which is highly reflective. (Other companies mylar and reflective rate is as low as 50%, and it won't provide efficient light. They use thick 1680D oxford canvas, and being double stitched, it is tear proof and perfect for locking in the light. Their grow tent keeps 100% of the light inside the tent and helps your plants absorb more light. EXTRA-THICK material means No light leaks or rips at all.👍 Strong Stable metal corner adapters and poles are the trustful supporters of the tent. It's very sturdy, neither rusting or paint flaking off. quick and easy tool-free setup. They use heavy-duty metal zippers, with double layer black lining, by far the best on the market which creates a light-proof seal. The Easy observation windows are made by double layers of cloth. Observe the growing conditions of your plants without opening the tent and avoid disturbing your plants. The observation windows also serves to dissipate heat and ventilate the air.

Se hice el cambio de fotoperiodo a 12 horas de luz y 12 de oscuridad , se aplico tambien poda de bajos y se cortaron algunas hojas que estaban estorbando

OMG the smells are becoming so intense, from lemon to the sweet caramel mix with chilies and a touch of pine trees, i think i cant put in words what im smelling and were is taking my mind, but i can tel this, what an amazing combination of fragrances that are flying around The tricomes are shouting up as they become fatter and frostier, all cristal clear so far, i think i still have 3 mb 4 more weeks to harvest, lets see 😜 Just calculate my VPD and it’s 0.98 kPa need to increase this up to 1.2 for now s i’m moving my ligth up a bit and see if it works 🙏🤓🙏 Thank you all for following, comment, like and all 🙏 100 likes 😅🙏 🙌🙌🙌🙌🙌🙌🙌🙌 ❤️❤️❤️❤️ Loving this LED Tec 😍 Girls: 1-BlueBerry 2-Alaskan Purple 3-Poyote Gorilla 4-Hindu Kush 5-Whitw Mango 6-Super Glue 7-Badazz Cookies 8-S.A.D. tent -8x8 / 2.4x2.4 but i'm only using 1/2 so 4x4 / 1.2x1.2 Led - Lumatek 465w Compact Pro at 100% All i Grow is medicine for myself, Stay safe, stay tuned and B Happy Peace out D

Día 72 (18/12/2023) Empiezan las temperaturas frías... (tengo las luces encendidas de 20:00 a 8:00...) Día 74 (20/12/2023) Cogollos engordando y poniéndose duros a velocidad de crucero Los colores morados de esta cepa son cada vez más profundos 😍 Día 75 (21/12/2023) Riego: 1 litro H2O + Melaza 1 ml / l + Alga Bloom 3,5 ml / l + Sugar Royal 1 ml / l + Power Buds 1 ml / l + Green Sensation 1 ml / l - pH 6,24 - 875 PPMs Desde esta semana, añado nutrientes Cal-Mag Amino 1 ml/l y Enzymes 2 ml/l de XpertNutrients Día 76 (22/12/2023) Nuestra planta se queda sola en la tienda porque su compañera se ha vuelto hermie... Espero no la haya polinizado Relleno la parte superior del sustrato con 3 cm de Light Mix, ya que se ha compactado mucho Riego muy despacio con 500 ml de H2O para que se asiente Día 78 (24/12/2023) Reviso diariamente en espera que no sea hermie o se haya polinizado De momento todo va bien.... Añado un par de vídeos para que veáis los espectaculares colores morados que están mostrando 😍 En 20 años cultivando, nunca había visto una cepa tan bonita. Im in love 😂

Day 79: Watered the gorilla cookies with 1.5L with nuts 1481 ppm, 3151 us/cm, 3.1 EC (last week before flush)(same feeding as last week on the diary) 283 ppm, 591 us/cm 0.5 EC (3 strawberry banana, 3 purple punch and 3 wedding Cheesecake) (2L each) Harvested 2 purple punch Day 82: Watered the gorilla cookies with 1.5L with nuts 1605 ppm, 3414 us/cm, 3.4 EC (last week before flush)(last feeding with nuts) 268 ppm, 561 us/cm 0.5 EC (3 strawberry banana, 3 purple punch and 3 wedding Cheesecake) (2L each) Day 84: Watered all the plants with 2L each 259 ppm, 551 us/cm 0.5 EC Day 86: Watered all the plants with 2L each 272 ppm, 578 us/cm 0.5 EC

Some good progress this week,plant 1 now starting to stack,plant two now showing proper pistils. Never had a plant flower so quick as plant 1,only around 15 days since first signs, owing to the new Voost VST 240 https://voost.com/ top L.E.D at unbeatable prices for such high spec,trust me😉 😎 Happy growing Nugz 🤞🏴󠁧󠁢󠁷󠁬󠁳󠁿🍁 🍁🍁🍁🍁 Breeder-White label purple bud 🍁🍁🍁🍁 Light-Voost VST 240 https://voost.com/ (code TightNugs 7.5% off orders over £200 excludes any sales.

Vorblüten. Insgesamt wirken sie gesund, mit kräftig grünen Blättern und ohne Anzeichen von Mangel oder Stress. Die Beleuchtung läuft nun im 12/12-Zyklus. Der Abstand zur Lampe wird regelmäßig kontrolliert und angepasst, um Verbrennungen zu vermeiden. Die Temperaturen liegen tagsüber bei etwa 26 - 28 Grad, nachts bei 18–20 Grad. Die Luftfeuchtigkeit pendelt sich bei etwa 45 bis 50 Prozent ein. Die Pflanzen werden alle zwei bis drei Tage nach Bedarf gegossen. Diese Woche gab es die erste Gabe Blütedünger mit reduziertem Stickstoffanteil und mehr Phosphor und Kalium. EC- und pH-Werte liegen im optimalen Bereich. Ein Schädlingscheck verlief ohne Befund. Alles läuft reibungslos.

Well here we are at the beginning of week 8. Super windy today but I got my new rain tarp up. June 25th gave all the plants pretty good snip except for Cinderella number 2 which is still slow but coming along tomorrow I may top all the other little branches not quite sure yet. just fucked up my pH metre by hitting the calibration button by accident and now I have no solution to recalibrate it so I'm going to have to wait and kind of wing it until the new one gets here in a couple of weeks but my pH in my house water is around 7- 7.5 12 ml of vinegar in 12 qts should be the ticket. Friday June 26th well I found a way to use clothes pins to use them as wait to hold the branches down I kind of like it tell me what you think. well looks like everything is coming along okay I keep the newts down I rather have them a little too lower than too high. well this is the last day of week 8 in July 1st and everything seemed to be going along fine but it looks like my Cinderella 99 run into a bit of a deficiency on the nitrogen and somebody let me know if this is right or not and if there's somebody in Toronto who can test the tap water for pH levels and let me know my pH metre is broken right now

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

DAY 33 Vandaag ben ik begonnen met bloei voeding. De plantjes zien er goed uit. Zou het verstandig zijn om wat bladeren te knippen ? graag hoor ik jullie mening alvast bedankt.

So you've grown a normal seed? so you've grown and autoflower? well why don't you try the fast streams definitely one of the most interesting and exciting grow experiences I have been a part of up-to-date. One of the most notable traits was the branches being extremely susceptible to bending making it a truly ideal strain for a SCROG. I think one of my main mistakes was leaving the plants in a 3 gallon pot I would suggest that nothing less than a 5 gallon pot should be used and from what I have seen going from jiffy transplanting into a Solo cup then solo cup straight into a 5 gallon pot has worked much better for the clone run I'm doing now. These plants need support!!! That is a must! Especially if you are going to use defoliation at any point you're gonna see a mass of spurt of growth. Now another thing I noticed is that the end right towards your finish window where your trichome are going from clear to Cloudy They tend to mature a little bit faster than a normal photo sensitive Strain. On the feedings I would do at the end 75 to 80% of the recommended maximum nutrient mixture you are using meaning use a little bit less the nutrients for the green crack. Expect A flowering stretch of at least 75%. Other than that I'm extremely impressed with the genetics again that humbled seed organization has created for us cannabis lovers to Excite and motivate us to have something different.

Transplanted at the beginning of the week, it did a lot of good for them 🌱💪

11.12.25 Heute wird die blüte eingeleitet und ein paar Fotos folgen noch. Zu sagen gibt's nicht viel nur das sie sehr gut aussehen, alle Tops auf einer Höhe sind und die seitentriebe auch bereits neue seitentriebe bilden. An 2 Stelle ist mir vor n paar Tagen ein trieb abgebrochen aber innerhalb der paar Tage sind schon 2 dafür nachgewachsen aus dem Stück was noch hing. 13.12.15 Hab erstmal nur die Bilder hochgeladen hab im moment nur wenig zeit ✌️ Wie immer würde ich mich über Tipps freuen ✌️🍀

5/25 this plant is starting to gain some vigor finally. Has some real nice healthy color and I'm happy with where she is and/or where she will go once flowering commences. I was worried that she wasn't going to grow into much of anything. This seedling has been fighting since day 5/26 top dressed. She's taking off Also her very main top, keeps branching off weird, it's like 3 at a node almost. Its been having weird mutations like parts of leaves missing and odd shaping of

Week 6 is flush week for this amazing girl Snow Panda she is very frosty an dense and super sticky smelling amazing an looking just as good this is one of my favorite girls so far

1rq semana en floracion.

12 hours max soaking before directly going into 18ltr pots. Amazing germination speed and rate as always from the guys @ 420FB

cómo ven pode todas las hojas bajas, y va creciendo mejor