hola colegas , 6 semanas desde el cambio a 12/12 , las nena siguen bien amarillando algunas hojas por falta de nitrigeno me imagino , instale los tutores que me faltaban a las 5 nenas que no lo tenian , EXHALE CO2 es una bomba 100% recomendado. tienen un olor muy rico que al empezar a subir la escalera del segundo piso se siente el buquetazo , segun yo voy bien , cada vez queda menos para la cosecha y eso me deja muy feliz , ya quiero que llegue ese dia , el ultimo riego con fertilizantes que hice no use la base (top bloom ) y la verdad respondieron bastante bien las plantitas , saludos a tod@s de esta gran comunidad y pagina , grow diaries ,me encanta. pueden ver el avanze de mis nenas por instagram tambien , abrazos y que esto no pare .

Brought to u by Mars Hydro SP3000 x2. Grow on Earth. Grow with Mars. All pics day 40. Welcome back. Mid/late flower now. Winding down nutes and teas and will feed to get the buds to ripen. Apex is frosting up nicely and her smell is popping, closest I can get to describing it is like cleaning products from a real fancy hotel…but lots of fan leaves are showing signs of deficiencies and/or lockout cuz she’s root bound really bad. Buds and sugar leaves aren’t affected, so far so good, I hope they stay that way 🤞 Gelato is fattening up real nice and has that distinct pungent aroma but not as strong as apex and not a lot of trichs. I hear this strain starts picking up in week 7+…although she’s root bound too but not as bad (I think). Lights are at 80%…they’re fricken beasts and way too much power for such a short, tiny tent. Next grow will be same strains, bigger final pots and will be in a new tent that is 6” (15cm) taller, every little bit helps :) That’s it for this week. Thanks for stopping by. Stay safe and happy gardening 🌱🌱

Day 64 (First Grow, Flowering Phase): Hey everyone! We’re on day 64, and the flowering phase is in full swing. The buds are forming beautifully, and the plants have grown 5-10 cm above the SCROG net, creating a lush canopy. The humidity is steady at around 50% or lower, which is ideal for this stage. To recap, we've been maintaining an even canopy with the SCROG net and our Timor shield mantis continues to keep pests at bay naturally. Our plants are thriving, thanks to the even light distribution and careful humidity control. We're considering installing a second fan in a few weeks to improve airflow and potentially adding a second SCROG net to support the plants as they continue to stretch during flowering. This should help stabilize the plants and ensure they get the support they need as the buds get heavier. Any tips or advice for the next steps? Let us know in the comments!

Everything’s looking great! She’s starting to gain speed a bit now hopefully she’ll fatten up, still have a lil bit to go..thanks for tuning in

This week I will maintain the same techniques from previous week. Noticed that main leafs got bigger during the weekend, both plant look healthy and happy and are now looking wider. Also to note that I have been rotating the vases so they catch light in different positions/sides. It does not replace the real sun movement however it will stimulate the growth in different parts of the plant. Hopefull by the end of this week we will have a new set of baby leafs.

Hey there, Welcome to another week in the tent. All plants are proceeding well, only thing is the gelato leaves are very light green in places bordering on yellow. I have added a little exra N nutes to that plant to try and remedy. Other than that its plain sailing. Very easy going plants. Might just be me, but im sure that the QB has bought out the pistils sooner than the HPS did, but who knows. Light is doing very well, ecstatic with the upgrade. Tried to upload a vid, but getting site errors, so maybe later it will be up. Happy Growing.

Harvested at day 76, after 72h of darkness. Very good yield! 3.3 pounds of dry buds + 1 pound of trim The 2 keepers yielded 366 and 342g of premium quality smoke. Very uplifting and energizing high :) Love it!

Been wanting to grow this strain for a while now so many people have grown it and it had huge buds I’m excited. Everything I have grown from fastbuds so far grows really well

This is first week. i cover those plant with plastic glass. because from my expierence. from the old day. there are many enermy of your plant such mouse or many insect can get in your grow tent. so it's bad thing for me when the rat in my house come and ate all of my little plant when it age 1 weeks. so it should not happen again. before your plant strong. this state of plant is weakest of your plant. this time must very be careful.

- LAS PLANTAS SIGUEN CON EL MISMO PLAN NUTRICIONAL - NO SE VEN CARENCIAS NI EXCESOS - el hps de 600 MANTIENE MI AMBIENTE SIN MAYORES INCONVENIENTES -NO SE VEN PLAGAS NI MAYORES PROBLEMAS

5th week. Well. its time to start pre-flowering stage. Next 2 days will be 18/6 light period. then 2 days 16/8, then 3 days 14/10, then 12/12. Total width 25cm. Do you like my videos about grow? Please let me know -> leave your comments. 4-20 Brothers! 😎 32day - 4 november - second day of 16/8 light period. guys ! I'm growing a real mini jungle😜 Width is 29cm. the total width of the pot is 35cm. Next 3 days will be 14/10 light period. 34 day - 14/10 switched on

👉Alrighty Then👈 This plant finished out just around 90 days from seed 👉Puffed a bunch of it , and its exactly what i had hoped for i love the WC 👈 the buds are tight and smoothered in resin 👌 I have done at least 50% of the FB Catalog and this has become one of my favorites of there's....... Would definitely grow this one again and would recommend 😳 Persian Pie from Greenhouseseeds Full Gas from Greenhouseseeds Babba Kush from Greenhouseseeds Rainbow Melon from Fastbuds Papaya Sherbet from Fastbuds Weddingcheesecake FF From Fastbuds Purple Oreoz F1 From Seedsman Slurricane From Premium Cultivars Soil by Promix Nutrients by Cronks This was a crap ton fun 🙃 Thanks to all my growmies out there for stopping by its much appreciated 👈 👉Happy Growing👈

Slow growth this week in my opinion. The dosage of fertilizer was increased to 2 tablespoons. Let's see how that goes during the week.

This week I have topped for a 2nd time with auto's it is a very tight window to train correctly. Now have 4 main nodes..mostly LST and light defoliation going forward

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Started the good and hot season with an adjustment refilling of soil the pot of the little one and giving more to the other two. Incredible grow in two weeks going from being near dead to being big as the other two.

Everything is going great. I transplanted to final pots. 5 gallons. Turned on the 400w mh and getting excited. No complaints, no problems.