It’s not getting attention. But I water it when it’s in need but I’m not sure how much light it gets. Putting the F1 Milkyway to the test! I may have to try it in a bigger pot at some time if it delivers under these circumstances. Update: While I lightburned my other plants, this must have been in a better position than i first thought. But it cost me alot on the other plants I got :/

170g dried buds Mars hydro TS-1000 Increase Yield & Crop Quality in Led Grow Newest SMD Led technology provides the highest Par/Lumen output, makes you get 30% higher yield compare old led lights, can get up to 2.5g/watt yield. Only 150 watts true output so you don't have to check your bill every month. 342 pcs LEDs, more scientifically and energy-efficient! Perfect for 3'x3' (100x100cm) veg stage and 2'x2' (60x60cm) bloom stage https://www.instagram.com/marshydro_aliexpress2/ Peace & love - L.S.T🌟

That’s a tall skunk.

Ok, so I forgot to take any pics on week two and holiday time week 3 came up real fast. so we are going to skip over any of the week two details. just watered em and they grew. This week we did a nice little transplant into their final 2gal pots. Fortified with a bunch of amendments and Acti-Sol, everything is listed in the video. Any question just let me know. I don't age my medium at all and I think I should start doing this to avoid some other things, I just find it hard to do it in winter time indoors as soil can be stinky with amendments and I like it to stay in the tent under the carbon filter. Medium I used is 40% coir, 30% perlite, 20% compost, 10% vermiculite and 10% worm castings. Then the amendments are added into that at the rate of 30-60ml per 1Gal of medium. This batch has Gaia 4-4-4, Gaia Insect Frass, Giaia Diatomaceous Earth, Gaia Rock Dust, Acti-Sol Seaweed Meal, Acti-Sol Shrimp/Crab Meal, Acti-Sol 4-3-2, Acti-Sol 4-6-8 and Bokashi. Nice little mix, water it in with a bit of magnesium and microbes dissolved into the water. The bokashi should help break everything down and make a bit of a acidic environment for all the meals and rock dust to become available. Roots looked good, not a dense as some runs but still nice. I feel the soil might be a bit hot so will expect some burnage on them hopefully its nothing to crazy. Will ph down with a mix of citric, malic and ascorbic acids. They are in a rate of something like 50-60%/40-30%/5-10%, its written down in my of my notes just can't find the pad ATM. Also more videos vs anything this week. I like em more but processing them is annoying AF. Side note, my lights timer was on 12/12 not 16/8 so it explains why they went into bloom, switch it back and will top the plants next week one they start to reverse their ways and get comfortable in the new pots. Also got ride of the chive seed start as it was looking sad, plus I got a bunch of European chive varieties in the mail yesterday and want to try them out. see if they really have any different taste and what ones I like better. Till next week.

Hello everyone 😎 Week 4 for my girl She is doing very well,growing at fast pace and with a beautiful green colour on the leaves. For the feeding schedule half grow half bloom + 1ml/L power buds Have a nice day 😎

D29/V25 - 29/04/23 - Benting D30/V26 - 30/04/23 - EC 0.9 pH 6.5 D31/V27 - 01/05/23 - LST and Benting D32/V28 - 02/05/23 - Some other LST D33/V29 - 03/05/23 - Added water and nutes - EC=0,9 pH=6,5 D34/V30 - 04/05/23 - LST D35/V31 - 05/05/23 - Nothing

Went from lights out for 3 days started to bloom the clones then had to get them right and we'll here we go again lost 5 but welcome to the north east ✌️

Today the final day. The time is come to cut down and harvest. Everything looks great. I will report more in 2 weeks.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Tag 56 - Beginn der 9 Woche. Die Blüten entwickeln sich weiterhin gut, sie sind schön frostig und duften stark und süßlich. Leider wurden die Blätter immer gelber und fallen teilweise ab. Für mich ist das anhand der Symptome diese Woche eindeutig geworden, dass die Pflanze hier mit zu viel Licht und Hitze zu kämpfen hat. Leider kann ich die Lampe nicht weiter hoch stellen ohne das es die andere Pflanze beeinflussen würde. Deshalb habe ich das Düngeschema nicht geändert und weiterhin Cal-Mag dazu gegeben damit sich die Pflanze vor der Lichtintensität und der Hitze besser schützen kann. Gedüngt wurde an Tag 51 der letzten Woche. Nährstofflösung: 1 ml/l - Sensi Cal-Mag Xtra Advanced Nutrients. 1 ml/l - Big Bud Advanced Nutrients 1 ml/l - Bud Candy Advanced Nutrients. 1 ml/l - B-52 Advanced Nutrients. 2 ml/l - pH Perfect Sensi Bloom Part A. 2 ml/l - pH Perfect Sensi Bloom Part B.

Top Gun #2 finishing up nicely, checking for mold every day, humidity is back in the 50s. I just trimmed up Top Gun #1 yesterday. 9 day dry, humidity started dropping into the 40s the last couple of days. Only one other tip of the buds had some mold on it. I’m smoking it idc🤷. Total dry weight was 50grams minus the molded buds, I’ll make butter with those. Small plant but smoking it right after drying it was one of the best things I’ve tried, very proud.

These girls are on fire , super dense heavy nugs . Smells incredible and can’t wait . Harvest is expected before the end of the month . Btw this is actually my first grow … I’ve been studying a lot and have certs from thc university.

Going to do some dark time before the flip. Should boost the phytochrome A before starting the 12/12 schedule. Opened up some new growth with the stress clips. Time to flower for my first time! I’m very excited to learn this stage! Any comments please add thanks all!

Hello everyone! here it is, the time has come to spend all this beautiful world in bloom ... although one of the small is typically indica, the others int a more sativa phenotype, there is just a look at the leaves .... here I water this week with plagron terra grow at a rate of 5ml per liter ... here is now room for photos ... peace ...

week 8 for the F008 we will harvest this girl in some days, looking good, my clear favorite from the test strains. nice and gassy, she smells like pure diesel strain, amazing terpenes and also a lot of thc! nice ball shaped buds and very dense, hard as rocks!

7th week is here. They are good looking girls! Some weeks left to be finished ! Water 100 ml 2x a day per plant. Fertilization on Monday, Wednesday, Friday and Saturday with the same amount of water. Light schedule 20/4 with 600 W and ventilation goes on when the lamp works. Day 52: All good, some yellow leaves can be seen at the bottom of the biggest plant it matures quicker than the others I think it started using the energy out of the lower leaves to produce more buds. I will remove it later. Day 55: I couldn't come yesterday so my friend did water them. I am here now in the morning ing. Flowers developing nicely among the stamps. The bigges one start getting yellow it matures quicker than the other ones. Day 56: I removed unwanted yellow and dead leaves and also tied the plant from different angles to get more light. They look good now, they are spread. Plants are really nice and the flowers keep coming! Now it is gonna be better air flowing and the light also reach deeper parts at the bottom and all over the plants!

Had rain For 2 days high humidity buds also added another wave of pistills on 1 cheese strain