I think a beginner can grow this plant easy because of its resistance and the fact that doesn’t need much care.If you make it to the end you will get incredible result for the first time.

The Sin è una di quelle piante che crescono sorprendentemente, ho beccato un fenotipo veramente mostruoso ed è un peccato non poterlo crescere al sole 🌞, ha raggiunto Calypso Sun Rock in vigore, la scorsa settimana non era così veloce e ha fatto passi da gigante, ci vediamo nei prossimi aggiornamenti 😃👌✨🌱💥💚🎶🎬

Weekly update on this amazing strain. Theze lovly ladies really surprised me this week. They're turning out to be some great looking plants. Veg was a little worrisome but I'll take it. All in all Happy Growing

Think these ladys are gonna be big 🤔

5 week was very intense, it showed itself in all its glory. Every day he rejoiced in his delightful forms, and in the middle of the week he had great tension, which, in my opinion, should have led to self-development, stress, a slight light burn. The exhaust fan burned out, I had to install a cooler from the system unit. All that is needed is all that is needed to get the maximum light, and practically every branch and leaf is light, nothing interferes, and the light penetrates into the depths! I want it to be very fast))) I plan to translate it in color for 6-7 weeks, she got a lot of stress, which, in my opinion, is not desirable, naturally, I am not surprised, and it will tell on the harvest . I already have cultivation experience. imitate how an auto color behaves) in general, a high nitrogen content, temperatures over 26 degrees reach 30 degrees at most, considering the variety, not the best GHE fertilizer, light ventilation and my cheap LED lamp, on the fifth week you can get what you see. I am from the first experience that the miser pays twice! Comfort for plants, well, waiting for the first week of flowering, go ahead!

Eight weeks of flowering I raised the PH to 6.3 and the plants that showed phosphorus deficiency no longer presented the problem, the absorption must have improved since the fertilization was the same. But as not everything is wonderful in this life in the last week here in the city where I live we had a great cold front 10º C. Inside the grow the temperature varied at least 13.5ºC during the night and light off and 20º during the day with the light on, the cold always stops growth but the formation of buds is going well apparently. Definitely plant # B3 is either photoperiod or locked, it has white pistils on the stem indicating pre-flowering but even before entering the flowering period I don't know what to do lol.

Yo guys whats up Check out my videos and lemme know Im trying my best to keep up MY girls are doing very good Im just too busy right now with my son He is 10 months old now and sometimes I have to leave behind the girls thank God I had built over the past months a great setup and I keep improving every time I have budget so it is very kind of you every like you give and every Comment is a blessings for us as a family you guys help us a lot to continue promoting and growing the best medicine in this world, thanks brothers happy growing and keep enjoying our diaries we will keep posting once we get the time and space for sure stay tuned Now this PPM 1000 lowering down to 800/600/400 Until we are done for the remaining weeks Also we stop adding nutes for like 2 weeks now or so We only add water every Day Like 300ml at least to keep them hydrated But for us is best to add one 1.5 liters then wait for them to drain and leave them like that for like 3-4 days until water in the bottom is full absorb this forces the roots to go and drink water which makes the plant grow bigger... I know THESE are autos but you would be impress depending on the genetics of what they're capable of The only thing that really f them up is time They dont go back just forward so yeah Got to be quick Anyways how you doing guys Happy growing thanks for everything

Hi all👨‍🌾👋 Welcome to my another week update Hope everyone doing well 🧑‍🌾🤤 Week 3 Feb 16 - Feb 22 It was a very easy week. Both baby girls are growing steady but not in rapid speed yet. 2 waterings of 500 ml each on Feb 17th and Feb 22nd alongside with foliar feedings(100ml water and fish mix) both looking healthy and happy. LST is coming next 😁🧑‍🌾✨🍀 Wishing you all a wonderful week✨🍀 Much appreciate all your likes, follows and comments. 🙏💚❤️💜 Peace and love brothers and sisters 👨‍🌾✌️💚 Links https://2fast4buds.com/seeds/TROPICANA-COOKIES-AUTO https://www.biobizz.com/ https://fishheadfarms.com/

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

D71/FD43 - Sooo...its all gone a bit to dick this week! So, as noted before, Oscillating RAM fans keep blowing on me. Just stop dead and haven't got a chance to get down the 90miles to get them replaced again. Well, noticed a few leaves that looked like they may be a bit light coloured...only 1 or 2, at the top buds which is strange and not really an N deficiency...so I looked more closely....4 buds with the dreaded fur on them!!! Fucking Bud Rot!!! Ones closest to the door that would get the biggest temp differential (So the weirdest humidity) and no air really getting round them!!! Talk about a SICKNER! This grow just seems to be blighted (literally) with something new every 3 weeks!!! So, had to chop a few tops and now going to be on the defensive for the next few weeks. We're not yet ripe and *hoping* as these had the least air movement round them, the spores didn't get about the place!!! Worst as well is the height of all the plants...cant get the UVB bulb in there for fear of burning them but that would potentially sterilise the fungus (I'd usually be using a 10K finisher this week but with the change to LED...) so now to work out what to do. Also didn't help that I didn't put the lid on my tank right last night doing a check and think i pumped about 10L of water about the place!!! Yip, today was a disaster!!! So, apart from that, I've dropped the Silicon and changed to TNC bactorr for my root inoculant (Out of great white). Apart from that, happy growing all!!! 💪

The strain is really strong to various stress, it's felt a difference between the two phenotipes, their are both delicious but one more berry flavoured and the other more exotic one. At the moment the best strain I've ever grow! 💐🍧

Day 54 from seed started running into some issues,leafs are turning pale with brown spots some greyish spots tips curling upwards shes growing but nugs not as dense as expected shes very short

In the home stright now, a few milky trichomes on each plant, just ph’d water for the next 1-2 weeks. The smell from these two is phenomenal, its actually making it through my carbon filter.

Week 2 flowering and tropicana cookies very easy to grow and she loves feeding o feed her with high dose and seem she love it all time I feed her every 2days and I wanna feed less water and do it every day when light goes on

Day 78: I checked the trichomes today and I'm pretty sure I saw cloudy ones. Hard to say even with the microscope, but I decided to wait at least until tomorrow before flushing. So I fed them today with the same solution as the previous week. Day 79: I saw more than one cloudy trichomes this morning so the final flush was given. This should, SHOULD, now be a smooth ride until the end. The buds are stacked, packed with trichomes and... damn that smell! Day 80: Nothing to read Day 81: Nothing to read Day 82: First watering since the flush with tap water, nothing else to read. Day 83: Nothing to read Day 84: I don't think they'll all be ready at the same time. I think they'll go in clockwise order starting with the bottom left one.

week intel: we reached the peak of flowering stage and need to cause a little more e.c stress from this week so e.c stress is twice a week i reduced the amount of Nitrogen and calcium and stopped feeding silicate in other hand i raised the amount of base nutrient and booster short story version: more K & P - less N stresses : a little E.C stress around 1 and 1.2 twice a week from this week feeding: i feed them 3 times this week with this order : day 1 : i feed them high with base nutrients(calcium & micros (half dose) + Bloom) about 566 ppm - 1.1 e.c to cause a little stress. day 3 : i feed them low dose of Top-Max + B-52 around 392 ppm - 0.7 e.c to let them recover a little but not fully recover still a little stress will caused. day 5 : i feed them high dose of Feeding Booster around 630 ppm - 1.2 e.c to cause e.c stress again guide of the week : e.c stress if done correctly is one of the very few ways to increase quality in all aspects ( color , aroma , taste , bud structure , resin contents ) but if you over do it , listen carefully brother : it can reduce quality in every aspect so always look for signs and never reach the red line

Great cultivar

Grow is coming along well, though the Black Devil is still lagging behind IMO. I think the strain maybe isn't too impressed with being Main-Lined? Either way she's hungry so I gave her some bokashi, clover leaf trim and worm castings. Next week is main dry amendment feeding so the girls will get everything they need. Couple more weeks and the Northern light should make it's way around the entire pot, then if it's tall enough, I'm putting them into flower. Some weird shit on the microscope today; videos above.

Happy End