~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_ 1/15/22 😻Week 5 of veg and things are looking good! I didn't know how I wanted to train these so my husband's suggested we top 2 plants and low-stress the other 2.. I went a little too heavy with their last feeding, no burned tips yet but 2 of them are very dark, we'll be doing water only for these this week..there is some minor discoloration on a couple of the lower leaves nothing major but something we're watching..we'll update again midweek..thanks for all the love Growmies ❤️🌱 ⚡Mars Hydro/SP-3000⚡ Specifications ⚙️: Diodes: Samsung LM301B / Osram 660nm (960 total!) Driver: Meanwell 300watt 🔌 (300W±5% @AC120V-277V) PPF: 824umol/S ☢️ PPE: 2.8 µmol/j 〰️〰️ Lifespan: >50k hrs ⌛ Weight: 10.1 lbs (4.6kg) Veg Coverage: 3 x 5 ft 🌱 Flowering Coverage: 2 x 4 ft 🌼 -The SP-3000 uses an aluminum heatsink (no fan) and the driver can be placed outside the tent 🌡️⬇️ -IP65 waterproof ratings, tolerant to high humidity grow environments 💦 .. -Up to 15 can be daisy-chained together and all controlled from a single light 💡~💡~💡~💡~💡 ~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_ 1/18/22 Things are good 😺👌..New growth seems to be coming in fast on the 2 plants we topped...much faster growth than we've seen up to this point..all plants seem happy and all have a great color (after working out my slight overfeeding )..we've been running the MarsHydro near the top of the tent the entire grow as a test and its still more than adequate. .we don't have a light meter but we do have some crazy tight node spacing to confirm lol...I still say these plants are a little on the small side but they seem to be growing faster now..I'm pretty sure leaving them rootbound in cups didn't help their development...I think they're finally becoming adjusted to their new homes and we'll see some explosive growth in the coming week 💡🌱... fingers crossed 😺🤞 ~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_ 01/19/22 AHHHHHH!!! I DID IT AGAIN!! Kicked my timer power after watering yesterday and put these into about 14hrs of darkness 😥.. lights are back on, hopefully they didn't notice lol.. ah well these things happen and I'm sure they'll be just fine ❤️🌱 ~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~

Hey guys so another couple weeks hav3 passed this lady's is coming along nicely . Started pre flower and stretch should come this week .

Mandarin XL Auto 🔸🔸🔸04/29/23🔸🔸🔸 Day 60 - Above Ground - Target 65 Days 📝Sunday 07.02.23 - This plant is looking very good, the buds are hard and very stinky, with deep citrus funk mixed with mango, anther 2 to 3 weeks left for her. 📝Wednesday 06.28.23 - The buds continue to pack on weight and ripen, the smell in the tent is amazing, fed with a Mayan tea from Humbolt Nutrients, loved the way it looked, they have great products. 🔸🔸🔸🔸🔸🔸🔸🔸🔸🔸 Mandarin XL Auto ◾Grown By: MrJones ◾Light Source - Medic Grow Smart 8 LED ◾Light Settings - @40% and V1 (Veg-1) ◾Soil - ProMix HP ◾INSTAGRAM - @medicgrow420 - @ganjafarmerseed - @gaiagreenorganics 🔸🔸🔸🔸🔸🔸🔸🔸🔸🔸 📝 Seed Details - Ganja Farmer Cream Mandarine XL Auto is a 4th generation auto-flowering strain. A tall and very productive plant created by combining Cream Mandarine Auto and Super Tai '98. It offers delicate, spicy notes of Super Tai wood and nuts and a sweet, citrus aroma of Cream Mandarine. Produces buds and resin abundantly. 🔸🔸🔸🔸🔸🔸🔸🔸🔸🔸

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

Well week two is going very well both phenos one and two of the purple lemonade are looking very healthy. Pheno Number one has very slight deformation from the seed sticking on the seedling leaves for a little too long. Nothing too serious. They have grown an inch vertically in the past week and I would say they are off to a great start still. Patiently enjoying the growth! Thank you for checking out my diary leave a comment and I'll come check out yours as well! ✌️

Day 92 (September 16th): so I’ve realized a PH issue in the soil… unfortunately it’s too late to make a drastic change, but I’ve lowered the PH in my water to try and level her out until harvest. This would explain my multitude of deficiencies I’ve been having. Still not sure how to better manage soil PH but you can be damn sure I’ll do my research so it won’t happen again. Soil is hard haha. Day 94: starting to see more and more amber trichomes. This will likely be my last week of flower. Should be ready around day 100. Let’s see what happens! Day 97: So I’m starting to think I was wrong… these may have more time than I thought still. I’m definitely okay with that. Hopefully they continue to produce and thicken up.

First of all we had quite some rainy days, which is almost impossible as in July this didn't happened for years, but of course i plant something outside and it automatically needs to rain...😡 Then some stupid worms decided to build a colony inside the plant and screwed a couple of leaves, i killed them all for being disrespectful to my plant, that will teach them a lesson... As last we celebrated the plants birthday and threw some firework around the city 🤣😝 it was a nice party, the plant is still recovering from the party For the rest all good, more and more tricomes are appearing and in around 2 weeks we are harvesting 😁👌

She went from 40cm to 73cm within 5 days. Week 5. Day 1

These plants are finally stable! I've stabilizrd the temperature and humidity. They appear a week behind where they should be. Two of the Candida plants look severely stunted in their growth. This is likely due to early novice mistakes. (The Candida plants are in the 10gallon Rhizopots). The two auto Sour Diesel's are strong and healthy. The Charlotte's Angel auto is doing quite well.

Everything went according to plan. From germination the plant displayed a very good development. With no training I believe she gave a very good yield. She always looked very strong with no signs of any risk.

Humidity was freaking high for a while in my area and I'm struggling with RH in the tent, I didn't want the big dehumidifier to work 24/7 but this led to appearance of mold on a tiny side cola. Lesson learned. I did cut everything above the cola and made sure the rest was OK. Continuous surveillance until harvest, that should happen in a few days. Trichomes are almost all milky and her smell is telling me she's ready 😎

Scrog

This week has been time to put on weight!! They are getting so heavy glad put in secondary scrog net. Have only been doing water so plants can just pull what they want our the soil they are starting to fade on their own. They have some amazing kinda blue tent to the buds. They have a gassy sweet smell to them! This will be the last week I feel but still see some clear heads and no amber so shall see over next week!!

Moved light to 3ft above plants. 400ppfd plants are reacting well. Day 17 moved lights back to 24 inches above and made sure DLI was 45. Huge growth increase.

Fimmed the mothers and accidently topped an Auto because I wasn't paying attention. No big deal, all plants super healthy and growing strong

really easy to grow. Had some issues in the begging being underfed and low ph issues. But still grew through all the struggles. Wasnt the biggest yield but wasn't the biggest plant. So wasn't expecting truly much. The purple pheno gave me a zip and half about. The other green one gave me a zip dry. Both smell lovely and can't wait. Will come back next week or so to update smoke report. Also fixing to make cannabutter from some of this can not wait ^_^ . Will grow it again but in a bigger pot maybe.

Some yellow leaves and some calcium def etc etc. All good, they are progressing well. Gave a feeding yesterday. Should have staked them probably we’ll see. Overall about 4 weeks left.