90x60x140 (Mars Hydro) FC-E4800 (Mars Hydro) Easy2go Aquavalve5 (Autopot) Living Soil (Demetearth) Bruce Banner (PEV Seed) Gorilla GG4 (Ganja Farmer) Cream Caramel (Sweet Seed) Gorilla Gelato (Ganja Farmer) Blueberry (00 Seed) Kalini Asia (Zamnezia) Biscotti Mintz (Barney's Farm) Blackberry Cake (Sensi Seed) Amsterdam Amnesia (Dutch Passion) Gelato Cookie (Ganja Farmer) Purple OG Punch (Ganja Farmer) Sweet ZZ (RQS) Rainbow Road (Paradise Seed) Substrat ~50L: 30L Super Light Mix Biotechnologie 18L coco 2L perlite 1,7kg lombricompost 350g guano de chauve-souris 350g Zéolithe 350g Basalte 170g guano vers de farine 170g biochar 0,7g Endomychorise glomus intraradices 0,7g Bacillus Amyloliquefaciens Paillage de luzerne alfalfa Arrosage avec 2ml/L de mélasse de canne 1x par semaine Pulvérisation avec 1% d’huile de neem

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

I deliberately sent my 2 ladies into flower 2 months earlier. roughly the 12/12h rule. Sometimes it was 10 hours in the sun, but sometimes 12 hours. In my region it will soon be rainy and cold. I'm toying with the idea of harvesting them before I catch mold or something else. The trichomes are all milky. About 15-20% amber colored.

Plant is darkend up a bit more and more hairs are truning darker. Seems to be ripening up sooner than expected. Smells great and frosty, love it. Seems to slowing down on drinking too. The humidity in the tent has dropped dramatically, can't say I mind. I have been watering a bit everyday and not waiting until the plant drys totaly out. Been giving nutrients every watering, seems to need it. Tomorrow I will give it a good soaking and leave it for a few days until I think it needs it. This may be the start of the flush. If I dont see any new hairs grow out in the next few days then its flush time for sure. Cheers

It's flower time!!! Really excited for this phase of the plant cycle, and love watching them develop. She's drinking more frequently right as I'm ready for s vacation! I setup s self watering drip system and the rest run was a success!! Below are the days of activity since the last update: Day 34 1 gal distilled water 1/2 tsp Cal-mag 8g Simpro bloom formula Day 37 1 gal distilled water 1/2 tsp Cal-mag 8g Simpro bloom formula Pulled 8 leaves adjusted LST Hope all my growmies has a wonderful rest of your weekend

🗓️ 6° WEEK FLO // DAY 36-42 (from switch) // DAY 113-119 (from dry seed) ⚡- Light: 30 cm / 250 watt; ⌛- Schedule: 12/12; 🌡️- 22° C - 65% RH average; 📑- PH 5.9 - EC 2.3. PH is dropping on the #2. I'll change the DWCs next week, meanwhile I'm trying to keep the PH in the right range; 💧- 7° DWC change; 🍔- Flowering blend: tap water EC 0.4 + Silic 0.5 ml/l + CalMag 1 ml/l + Hydro A-B 1.8 ml/l + Oligo Spectrum 2 ml/l + Green Sensation 1 ml/l; 🌱- Things are moving forward, both are gaining weight. 🆕- Last week I got the TrolMaster TCS-1 (@TrolMaster_Europe) and I started to track my grow box data. I will add these info once I harvest!

She looks great :) she started drinking more, which makes me happy :) this week she didn't get any food, she got cal mag as a preventive measure:) and Biobizz heaven 4ml/l for dessert :) which she really likes :) good luck to the breeders :)

Floraison +18 jours . Le stretch est plus important que sur le pied mère 😍 bizarre mais tant mieux 😍

6.02 Growth has picked up since raising the PH of my nutrient solution to 6.25-6.5. I also began foliar feeding at lights off with 5 ml/g of Cal-Mag Plus. I added .25 tsp/gallon of recharge when feeding today. It raised the PH of the solution to 7.0 and will monitor over the next 48 hours for results. 6.04 When I tied down the big plant I split the stems where they were topped the 2nd time. Taped them up and cinced with plant ties. 6.06 End of week 9. Installed my automatic watering manifold with hydro halos. I've been feeding 3 times/day for the past week, and I won't have time to hand water 5 or 6 times a day during flower. Will probably need 1 more full week of veg before I flip to 12-12.

Update of Goofiez 2 !! It’s incredible this strain is so strong and fast grow !! Stay tu ed

Added a Scrog net to try and get more light into the lower bud sites. AutoMazar is still looking droopy and her color is very weird, she’s also the shortest. Auto Ultimate is still the beast!! She’s building trichomes and Auto Daiquiri is super sticky and will be harvested first, she’s on all water from her on.

.

This was my harvest update^^

Nov. 12 - start of week 7 and it’s been busy. HAd to clip a few leaves out of er to expose some more bud sites. Been tying er back every other day and growth has really picked up. She’ll be ready to go as soon as her kush neighbour is recovered👍. All is well peeps and thanks for swingn in👊 Nov. 17 update - not much to report. ANother lst session to keep spreading her out. Looks like these girls are gonna fill up the cabinet well. Really happy with how she’s developed. NOW biding our time to the switch. Background This one is gonna be fimmed out and defo’d as a comparative to its manifolded kush sister. They’ll spend a first week or so under a cfl assembly while the last harvest dries out in the cabinet. This is our 2nd grow of an NL strain and this one should be typical of NL in terms of resilience. That said, she’s a former cup winner so we’re hopeful for supreme quality this time around. 👍 Gonna jack up the nute shed. and supplements heavy throughout the grow in the hopes of really testing the limits of my grow space (approx. 2sq/ft). With the coco medium we’re hoping for that 20% bump in yield as well. THANKS for check'n er out

Hi guys we are officially in the 5th Week of Flowering :) Ladies enjoying their time, nothing looks bad or suspicious at least 😄 Still giving every 3rd day Nutes and almost watering every day huh, its quite warm and stinky in the Tent! Cookies and cream and Somango didnt really had stretch at all but their growing way faster than those Bubba kush plants! See ya next week

I am really excited about this one, it's the culmination of my favorite plants. Star Cookie x (grapefruit male, sativa dominate ) x Captains Cake 06/18/2018 Posting pics of parent plants. Looking for my grape fruit male pics 06/19/2018 She is growing fast half inch at least since yesterday it's good to see her growing under natural Sunlight, no leggy growth,and standing up to the constant breeze around my home. Captains cake is a 91 girlscout cookie x White Fire Alien og (OF Rascal seeds i think?) breed by captains connection Maine. Star cookie is (OG Kush x Durban) x GDP. Topdawg seeds. 06/25/2018 Still in seedling stage . . .this will be interesting never started seed this late outside, hoping veg starts by mid week. 06/27/2018 Still in seedling stage,starting a lite feed with AACT Made from Tomato Tone at 1 tablespoon to 1 gallon h2o and 1 tablespoon of unsulphured molasses to multiply the microbes, get

Day 24 flower and holy frost! Sour Diesel is frosting up so much its crazy. The bud structure is abit smaller than all the others but man is it covered and look how much these girls stretched. Northern Lights isnt as frosty but has my favorite structure. Have alot of nice colas going and they are also starting to frost as well. Grandaddy Purp is surprising me in flower, this was the plant that's been the biggest and tallest the entire grow until now, it is the smallest. Its still stacking and prob going to be in flower longer than the others. I been hitting these girls with 800-900 ppfd at 18 inches from canopy and they seem to like it.