continues LST + Defoliation we'll see what happens next 😎

December 16 2018 day 84 from seed LOOKS GREAT

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

She Is growing really fast. Started as the smallest One, now Is really big and thick 😍😍 I think She love lst ❤️

Amazing to mix with water pH and EC easy to reach the desired point

Harvest day First day of dry 10/2/25

On to week 9, will be starting to flush this week I think🤔 , buds starting to get bigger and denser by the day now main cola on the gorilla glue especially dense! Both stinkin glad I got a decent carbon filter! Counting down the days now can’t wait! Happy growing!

¡ÚLTIMA SEMANA! Esta semana fue la última para estas preciosas 🍇PURPLE PUNCH🍇 todas han estado aumentando el peso de las flores estas dos semanas atrás, engordando hasta tal punto de doblarse enteras, tuve que agarrar dos fenotipos bastante pesados al armario. en total estuvieron 9 semanas en floración. De los 6 fenotipos x3 han cogido tonalidades muy bonitas como podéis ver en las fotos. ⬆️90cm ☀️Interior ⏳64 días 🏺11L 😶 Indica: 90% / Sativa: 10% 🚀THC: 25%

This strain took really well to LST and didn’t seem fazed by the training at all. She did become a really heavy drinker about two weeks or so into flower and she definitely seemed to want more nutes than usual. I believe I’ve got two more seeds so will be running this one again if the smoke is worth it.

🙏 Big thanks to Plagron and Zamnesia for putting on the Eternity Grow Cup 2025. This kind of platform means a lot to the community. The chance to push our grows, test out new techniques, and connect with other cultivators from all over has been inspiring. Appreciate the love and effort that went into making this event happen! Hope everyone enjoyed looking over my shoulder on this grow and best of luck to all participants! REVIEW - Runtz (fem., photo.) from Zamnesia: ⬛⬛⬛⬛⬛⬛⬛⬛⬜⬜ The seeds from Zamnesia germinated rapidly, rooted strongly, went wild in veg, and produced an ample amount of high-quality medicinal flower. The diary speaks for itself on that front so this space will be more of a phenotype review. The plants were labelled A, B, and C from left to right respectively in photos. A and B were the 'clasicaly-appearing' sativa-esque leaves while C was the more 'indica-appearing' phenotype. Although A and B appear the same qualitatively, they did produce substantially different amounts of flower (despite their DLI being near max out for the entire tent footprint without supplementing CO2, so I do not think just being in the middle attributed to this). Past their different yields, A and B also have vastly different profiles. A is a classic gelato/ice cream cooler/Z terps, and my personal favorite of the plants (wish I had taken a clone of this one instead). B is the most on point for what I think of with Runtz, classic Z terps but with a slight tropical/rotten/sweetness to it. C had the misfortune of being ready to chop a little earlier than the other girls so her profile is mostly just spicey kush now. I did observe intersex flower sites but given the entire context of the diary and how the plants performed, I have to assume it was user error. No pollination observed in any of the flowers, as zero additional intersex sites occurred. The distinction between A, B and C is what I would expect from a typical F1 cannabis seed. I do not enjoy growing from seed much but these girls were manageable. Add to that the relative stability of the genetics for a Runtz recreation, and well, these are good quality beans for a great price. Not only is Zamnesia responsive and available, their confidence speaks volumes. How many vendors would have an in-house Runtz, knowing how much of a genetic-gravity-well it is in, and be confident enough to let ~200 randos grow it and show it? REVIEW - Green Sensation from Plagron: ⬛⬛⬛⬛⬛⬛⬛⬛⬜⬜ I enjoyed using the green sensation, the NPK values on it make it versatile in application and a power-house in flower. I can't say I noticed anything better or dramatically improved, although comparing to Heavy 16 and asking for a marked improvement is big ask in my opinion. What I was able to do was seamlessly integrate the Green Sensation into my current routine. The additive stayed homogenous, was easily introduced into solutions with no adverse impact to the water contents or pH. Most importantly, the plants enjoyed the additive and it did accomplish the main goal of being able to wane off your primary line. In this respect, by using Green Sensation I was able to save resources by lowering my Heavy 16 inputs across the board approximately 50% and supplementing with Plagron's Green Sensation.

Super erster grow

Day 43: Watered each plant with 1L with nuts 1589 ppm, 3380 us/cm, 3.3 EC (purple punch and wedding cheesecake) 1563 ppm 3325 us/cm 3.3 EC (strawberry banana) 1476 ppm 3180 us/cm 3.1 EC (gorilla cookies) I have 3 different feedings for the 10 plants They look healthy Day 45: Watered each plant with 1L with nuts 1563 ppm, 3325 us/cm, 3.3 EC (purple punch, strawberry banana, wedding Cheesecake) 1286 ppm, 2765 us/cm, 2.7 EC (gorilla cookies) 2 different feedings for the 10 plants Day 47: Watered each plant with 1L with nuts 1584 ppm, 3380 us/cm, 3.3 EC (purple punch, strawberry banana, wedding Cheesecake) 1481 ppm, 3180 us/cm, 3.1 EC (gorilla cookies) 2 different feedings for the 10 plants

Hello Growmies, The final chapter of this incredible journey has unfolded, and it's time to share the bountiful harvest and reflections on the entire cultivation process. Trimming the plant was a joy, and with gentle fingers, I removed all the large fan leaves. The precision in this process revealed the true beauty of the buds, setting the stage for a smooth harvest. With care and gratitude, the plant was chopped, and the buds were hung to dry. The anticipation for what lies ahead only adds to the sense of accomplishment. The wet weight, excluding fan leaves, stands at an impressive 782 grams. The buds are massive, a testament to the efficacy of the nutrients used, particularly the Power Buds. The name seems apt, as the result is a collection of sticky, dense buds with subtle lemon notes and sweet pungent undertones. In the final moments, the plant unveiled some delightful purple hues. While I wish I could have allowed them to intensify further, it's a reminder that each grow is a learning experience. In hindsight, a slightly longer fading period would have accentuated those beautiful colors. We live and learn, and that's the essence of this journey. A heartfelt thank you to Plagron and Zamnesia for hosting this amazing contest. The quality of the products and the support throughout the journey have been invaluable. As this chapter concludes, I'm left with a sense of fulfillment and gratitude for the collective experience we've shared. The plant's growth, from seed to harvest, has been a testament to the art and science of cultivation. Feel free to check out my other adventures in my bigger environments, until then...happy growing! Stay Lifted Salokin

Topped and pruned this week. Probably too late. Definitely watered a lot less. Be fore I am certain that I Was drowning them

02.07.25. Day 77 2 phenos have dense buds the other 2 I think just needs time to catch up. The smell is amazing, sweet with slight citrus. Buds are sticky. Fresh reservoir change out today. Trichomes are looking cloudy. I think now will be good time to harvest, however I want to push them some more. I will begin flushing after this week, or if trichomes say other wise. 5.08.25. Day 80 Today I added a sound of bees buzzing (10hr youtube video) in the tent. I saw documentary (https://m.youtube.com/watch?v=E8SJlyrEDX0&t=8s) of plants where plants produced sweeter nectar when exposed to this low frequency noise compared to those that was not. It is said the plant does this in an effort to attract pollinator. I am not sure how much truth there is to it however it seems logical. In another section of the documentary the plant roots was also able to hear water. Anywhere the speaker was placed with same low frequency of water the roots grew in that direction. Amazing.

fast growing auto, overloaded on nutes with this one but she still produced beautiful buds

Day 78 - pheno 1 was cut down, id normally hang dry the whole plant but decided to do it in branches instead. Second pheno has been left in darkness as i haven't had the time to cut. Pheno one is hung up and drying.. Room Humidity 60/60. As soon as she's dry enough I'll finish trimming her up. Then put them into jars. Might use boveda 60% packs but I'll see. Smells strong! Will post a harvest when both plants have been dried fully an in jars. Hope you enjoy the videos and pictures. Stay tuned.

So.. still getting FATTy! I changed the cob, cause i needed to lower temperature.. Now im testing a QB x 150w LM301H + 3500k660IRUVBlue I just ran out of solo tek-bloom, so im using biobizz.

Planten groeien als kool. Wanneer de eerste bloemen vormen, dan ga ik ze ontbladeren. De planten krijgen melasse- en zeewiervoer en wat lavamolen. --------------------Durban----------------- Durban wietzaden komen oorspronkelijk uit Zuid-Afrika en zijn gekruist gedurende vele jaren in de jaren zeventig. Door al deze kruisingen heeft deze soort zich aangepast aan het Europese klimaat. De planten zijn bestand tegen wind, koude temperaturen en schimmels. Ze bestaat voornamelijk uit Sativa en heeft een bloeitijd van 8 tot 9 weken. Voor zowel de binnen- als de buitenkweek kun je een grote oogst met stevige toppen verwachten. Durban kan buiten een hoogte bereiken van 200-300 cm. Durban is een exclusieve Sativa-soort met een unieke, heerlijke geur. Deze soort staat bekend om zijn kruidige, wazige smaak met sporen van anijs, zoethout, citroen en kruidnagel. Dit heerlijke aroma is een van de redenen waarom zoveel kwekers keer op keer voor Durban kiezen. Eigenschappen van Durban wietzaden Hoog THC gehalte Exclusieve Sativa Hoge opbrengst Sterke soort Heerlijk aroma Informatie Durban wietzaden Bloeitijd: 8 – 9 weken Genetica: Durban x Original Thai 70% sativa, 30% indica Planthoogte buiten: 200 – 300 cm Oogstmaand buiten: van juni t/m oktober Opbrengst binnen: 450 – 650 gr/m² Opbrengst buiten: 200 – 700 gr/plant THC: 20% Link naar de shop: https://seedsgenetics.nl/product/durban-poison-gefeminiseerd/