Ho saltato una settimana per corbezzolo che è solo cresciuta con il ramo principale e senza laterali, si vede che ho cambiato luce troppo presto e anche lei, essendo che era la più piccola, è andata sempre cercando la luce…oltre questo ho sbagliato e ho dato troppa acqua 💦 qualche settimana fa e la pianta ha avuto tante carenze e altri problemi radicali…spero di recuperarla almeno un po’ ma parlando di resa mi metto il cuore in pace… procediamo e vediamo come evolve 🙂✨🌀🌱💚💥🤞

Planties are getting bushier :3 This week we focused on LST, providing as much light so smaller tops could reach the size of bigger ones. We raised the light cause NL started showing signs it didnt like being close to the light, but also saw some other issues, tips of canna leafs started to yellow, about a week and a half ago we fed them some nutrience, home made organic and also a mix. So now im not really sure from what caused the yellowing tips. Nutriante deficiancy? Nutrient burn? Light? Taking now a closer look, to see upcoming signs that would make this situation clearer. Apart from yellowing leafs, the smell(rrrlll goood) becomes more noticable, its nice coming to the tent, feels like youre high from growing nature :=) We topped speedy chille and took of yellowing leafs, she really liked it and is now growing 4 tops

Almost time for, lights, camera, action ! She will get a decent defoliation to show those beautiful flowers and then she is ready to be documented, what an exciting and stressful grow for me, not because of the plants but because of the ridiculous temperatures we reached this summer 🌞

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Flowering day 68 since the time change to 12/12 h. Hi everyone :-) . We arrived last week :-). The trichomes are 70% milky, 20% amber and 10% clear. The nutrients have been used up and the lady is allowed to stay a few days before she comes into the darkroom for 48 hours before the harvest. Of course there is an extra update for this :-). Otherwise the week was poured 3 times with 1.2 l. The tent was cleaned and all women checked. Have fun with the update and see you next time. stay healthy 🙏🏻 You can buy this Strain at https://www.amsterdamgenetics.com/product/super-silver-haze/ Type: Super Silver Haze ☝️🏼 Genetics: Haze x Skunk #1 x Northern Lights Type: 70% Sativa – 30% Indica 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green House Powder Feeding ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.5 - 5.8

Nice growth. It's been stormy here so they're a little stressed but will strengthen back up when the sunshine comes back out. I'm thinking, or at least hoping, this is a female 🤞 I topped every bud site on every plant, both indoors & outdoors.

week intel: im felling in love with them everything is good . this week was time of second net layer , the first layer was for spreading the branches and doing LST now the second layer is for supporting branches for later weeks when the buds gain weight and gets heavy the net will hold branch stand up without benting. stresses: im just gonna cause a little E.C stress to raise the terpens. i found their max tolerance is 1.9 E.C so im not gonna reach or get close to that amount again and beside this wont cause any more streses untill week 6 that will do second pruning and we are done with trainings then. feeding: as always im feeding plants 3 times per week with nutrients , no plain water at all, just with nutrients with adjusted p.h at this order : day 1 : i feed them heavy with silicate +base nutrients(calcium & micros + Bloom) and karbo about 950 ppm - 1.8 e.c to cause a little e.c stress. day 3 : i feed them low dose for start of Green House Feeding Booster around 267 ppm - 0.6 e.c alone cuz this bad boy is very strong. day 5 : i feed them with medium dose of Top-Max around 160 ppm - 0.3 e.c to let them recover the stresses and eat the previous nutrients untill next feed at next week and the next 2 days will rest and eat :D just remember never feed them plain water besides the first week of germination always feed water with nutrients to feed them right p.h every time and to avoid excess water problems, and let them eat the previous meal for 24 hours and dry the soil, so at total only feeding nutrients 3 times a week other days let them dry and rest

Día 29 (18/11) Subo a DLI 45 CBD Auto 20:1 #1 sigue sin mostrar las preflores... ¿será una auto tardía o será una fotoperiódica? Cada planta empieza a tomar su ritmo de riego personalizado, así que allá vamos: CBD Auto 20:1 #1 1000 ml CBD Auto 20:1 #2 0 ml LSD-25 Auto 750 ml OG Kush Auto 750 ml He observado que regar 1 litro de golpe provoca escorrentía (llevándose nutrientes del Super Soil), de modo que riego primero 750 ml, y a los 5-10 minutos añado 250 ml adicionales y el sustrato es capaz de absorberlo todo😁 Día 30 (19/11) CBD Auto 20:1 #1 sigue sin mostrar las preflores... Debido a que no para de crecer CBD Auto 20:1 #1, decido hacerle una buena defoliación y algo de Lollipopping. A ver como reacciona! Riego H2O RO CBD Auto 20:1 #1 750 ml CBD Auto 20:1 #2 750 ml LSD-25 Auto 500 ml OG Kush Auto 500 ml Día 31 (20/11) Por fin CBD 20:1 Auto #1 muestra las preflores (femeninas)! 😍 Parece que va a ser una Late Bloomer! OG Kush Auto están empezando a estirarse, parece que va a ser un monstruo! Riego H2O RO CBD Auto 20:1 #1 750 ml Día 32 (21/11) CBD Auto 20:1 #1 se muestra imparable en su crecimiento a pesar de la defoliación y el Lollipopping de hace dos días De modo que me decido a hacerle Supercropping! Dicen que NO se debe hacer Supercropping a las Autos, pero siempre me gusta experimentar y ver que ocurre. Come on! Un día muy divertido! 😁 Riego H2O RO CBD Auto 20:1 #1 750 ml CBD Auto 20:1 #2 500 ml LSD-25 Auto 750 ml OG Kush Auto 750 ml Día 33 (22/11) CBD Auto 20:1 #1 se ha recuperado estupendamente! No deja de crecer y estirar cada una de sus ramas! OG Kush Auto está estirándose muchísimo (4 cm / día), de modo que decido hacer LST a la cola principal para buscar una nivelación de la canopia Riego H2O RO CBD Auto 20:1 #1 750 ml CBD Auto 20:1 #2 0 ml LSD-25 Auto 0 ml OG Kush Auto 0 ml Día 34 (23/11) OG Kush Auto se ha recuperado muy bien del LST! 😍 La rama principal ya está apuntando hacia arriba y creciendo! El resto de ramas de OG Kush Auto empiezan a estirarse igualando la canopia Le aplico un ligero LST para espaciar y distribuir las ramas Riego H2O RO CBD Auto 20:1 #1 1000 ml CBD Auto 20:1 #2 750 ml LSD-25 Auto 750 ml OG Kush Auto 750 ml Día 35 (24/11) Lollipopping & Defoliation day! 😍💥💨😁 CBD Auto 20:1 #1 Lollipopping de todos los nodos y hojas quedan por debajo del plano "virtual" que marca el supercropping CBD Auto 20:1 #2 Defoliación de algunas hojas de abanico que tapan a cogollos inferiores LSD-25 Auto Defoliación de algunas hojas de abanico que tapan a cogollos inferiores & Lollipopping de una par de nodos de las 2 ramas inferiores OG Kush Auto Eliminación de las ramas del 1er nudo (eran enanas y débiles) y defoliación de algunas hojas de abanico que tapan a cogollos inferiores. Riego H2O RO CBD Auto 20:1 #1 1000 ml CBD Auto 20:1 #2 0 ml LSD-25 Auto 0 ml OG Kush Auto 0 ml 💦Nutrients by Bio Tabs - www.biotabs.nl/en/ 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en-us/products/pro-mix-hp-biofungicide-plus-mycorrhizae "GDBT420" 15% DISCOUNT code for the BIOTABS Webshop https://biotabs.nl/en/shop/

02/15/2021 In the first pic you will notice bottom growth which will become my new moms! At least 3 there now. Top is stretching nicely I will have to decide soon if I want to top her or not, I will know better next week. 02/17/2021 Got two cuts today maybe two more by next week. 3 for the garden, 1 for mother plant. Ps. She grew an inch and a half in two days, so all is well. 02/20/2021 Stretching up to 13.5 inches(34.9cm) almost 5" in less than a week, I think she is happy! On a side note lower growth will produce two more clones for summer garden. can't wait to see her flower! I will probably need to top as she should stretch 1.5-2 X's in flower stretch. Till next weekly update happy growing!🙏

Alright, these ladies are mid way through week 7 day 52 of bud. Just 3 days ago i started the flush stage for these plants even tho I’ve heard 1000x that it’s not necessary for organic gardens. I still do it just because. So these ladies got fed 2gal each of plain PH corrected water. With no sugars of any kind added. They all seem to be flushing out very well with most of their leafs turning yellow with purple on the tips. If you see any crumpled leafs it’s from last week when I noticed my soil PH had drifted to high. So I’ve been trying to correct it but these ladies are just about done so I’m not to worried. It looks like we got 1 week left on the Train Wreck and maybe 2 weeks on the OG and the Melato. The Train Wreck actually looks ready to chop down now but when I got up close with a jewel scope I saw that her trichome development is only at 50% cloudy and 47% clear with maybe around 3% amber. So I’m sure she will be ready by the day 58 mark but the OG and the Melato will probably go to around day 65. Anyways thanks for checking out the garden. be sure to check in on next weeks update when I harvest the Train Wreck🤙

Hey everyone 😃. This week is still going perfectly 😃👍. The trichomes have been checked (70% milky, 20% amber and 10% clear) so there is still some time until harvest :-). Let's start the week: Flowering day 56 Today the plant was checked for health. Flowering day 57 Today it was poured with 1.2 l, only with Clean Fruits so that you can use up your remaining nutrients 👍. Flowering day 58 The humidifier was refilled and the tent cleaned. Flowering day 59 Today was only controlled. Flowering day 60 The humidifier was refilled and the tent cleaned. Flowering day 61 Today was poured with 1 liter, and the trichomes checked, of course the tent was also cleaned. Flowering day 62 Today was cleaned, the plant cobtrolized for trichomes, mold and health. everything looks great 👍. And fresh osmosis water was filled in tanks for next week. The way the trichomes look like, is harvested in about 10-14 days 👌👍. Until the next update, I wish you all the best, stay healthy 🙏🏻 and let it grow 🍀👍 You can buy this Strain at https://thecaliconnection.com/seeds/girls-scout-cookies-34.html You can buy the fertilizer at https://www.greenbuzzliquids.com/ Type: Girls Scout Cookies ☝️🏼 Genetics: GSC Tint Mint 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green Buzz Liquids : Organic Grow Liquid Organic Bloom Liquid Organic more PK More Roots Fast Buds Humic Acid Plus Growzyme Big Fruits Clean Fruits Cal / Mag Organic Ph - Pulver ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 - 0.4 EC. Add Cal / Mag 2 ml per l water every 2 waterings . Ph with Organic Ph - Pulver to 5.8 .

Just watching her go…

With no doubt these plants have not produced much more quantity and density of buds due to the 2 serious mistakes I've made (those who have read the weeks will know what I mean). However, I can say that I am happy with the production obtained given the mistakes made. Between the 2 plants, the one that has produced more and generated the most dense buds has been the Gorilla with the Man-lining technique applied, it has also loosed a bit less weight drying than the non man-lining one. In comparison with the Money Makers with which they have grown the result is the same. Both Gorillas have each produced more than any of the Moneys comparing them 1 to 1. Also buds are a bit dense than the moneys, but none of them is really desde at all. *****ESPAÑOL***** Sin duda estas plantas no han producido mucha mas cantidad y densidad de cogollos debido a los 2 graves errores que he cometido (quien se haya leído las semanas sabrá a que me refiero). No obstante, puedo decir que estoy contento con la producción obtenida dados los errores cometidos. Entre las 2 plantas, la que más a producido y ha generado cogollos más densos ha sido la Gorilla con la técnica de Man-lining aplicada, y también ha perdido un poquito menos de peso en el secado que la otra. En comparación con las Money Maker junto a las que han crecido el resultado es el mismo. Las Gorillas han producido cada una más que cualquiera de las Moneys comparándolas 1 a 1. Así mismo, los cogollos de las Gorillas presentan un pelín más densidad que el de las Moneys, aunque realmente ninguna de las 4 plantas ha generado cogollos densos del todo.

Salutations, my fellow cultivators and botanical enthusiasts! Welcome to the much-anticipated Week 3 Flower Report for our radiant queen, Jealousy, in the Power Buds competition. The journey through the flowering phase is akin to watching a mesmerizing performance, and Jealousy, true to her name, has us all captivated. As we transition into the third week of flowering, the grow room has transformed into a sanctuary of delicate buds, and Jealousy, adorned in green jewels, is perpetually in prayer. It's like witnessing a botanical congregation, where each bud is a devout follower, reaching for the heavens in pursuit of the ultimate floral nirvana. Now, let's talk about the delay. The ticking clock of the contest deadline has kept us on our toes, and yet, Jealousy stands resilient. Despite the time constraints, she continues to unfurl her petals with grace, and I can't help but marvel at her determination. It's like a race against time where our green queen is sprinting toward the finish line with unwavering resolve. In the midst of this floral ballet, the clones, those green offshoots of our regal Jealousy, have rooted with astounding success. It's like witnessing the next generation of botanical royalty sprouting from the roots of their majestic ancestor. These rooted clones are not just a testament to Jealousy's genetic prowess but also a green insurance policy for the future. And let's not forget the continuous prayer pose that Jealousy maintains. This phenomenon, known as "praying leaves," where the foliage points upward toward the light source, is a visual testament to the plant's happiness and optimal conditions. It's like Jealousy is sending leafy blessings to the botanical gods, asking for a plentiful harvest. As we navigate the third week of flowering, let's extend our gratitude to Zamnesia and Plagron, the silent architects of our green utopia. Their contributions continue to shape the landscape of our horticultural adventure. A hearty applause to the Grow Diaries community – your shared experiences and collective wisdom are the threads that weave the fabric of our green journey. To my fellow contestants and growers, may your buds swell with abundance, and may the contest deadline bow down to the prowess of your green kingdom. Here's to a week of perpetual prayer, botanical grace, and the anticipation of a harvest that will leave us all in awe. Stay green, stay inspired, and keep cultivating those dreams! As always, thank you all for stopping by, for the love and for it all. This journey of mine would just not be the same without you guys. The love and support are very much appreciated, and I feel honored and blessed with you all in my life !!! With true love comes happiness. Always believe in yourself and always do things expecting nothing in return, with an open heart. Be a giver, and the universe will respond in ways you can’t even dream of . Friendly reminder: all you see here is pure research and for educational purposes only. Genetics - Jelousy @Zamnesia Nutrients @Plagron Light - @viparspectra P2000 Room size - 3x3 - 0,9x0,9

Welcome Back!💚 Die erste Blütewoche ist vorbei und die Pflanze entwickelt sich weiterhin prächtig. Die Blütenansätze sind schön erkennbar und die Pistilen bilden sich allmählich aus. Überraschenderweise wirken die Blätter sehr Sativa lästig, wobei genetisch eine Indica Dominanz vorherrschen müsste. Außerdem ist ihr Wuchsverhalten eher Indica dominiert. Heute war es auch Zeit den Green House Feeding Dünger einzubringen. Bin sehr gespannt, was am Ende raus kommt. Die Werte im Zelt sind optimal auf die Blüte eingestellt. ——————— 🌞 Temp: 24°C 🌚 Temp: 20 °C 💨 RH: 56% VPD: 0,97 kPa 😎PPFD: 830 mqm ——————— Stay Tuned! 💚

Not that much happening, I had some night temperature issues but that is fixed now with a heater I still have her on a 12/12 because she is in a room with 3 photoperiods, but in a week or 3 they will be switched to a 18/6 light cycle 👌🏻 Good growing everyoneee!

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

No surprises. Topping has been done at this point. Temperature and humidity controlled as much as possible. Some bending and light intensity increase. A 2x4 tent is actually too small to bring two plants to the height and spread that I would have liked. Ideal for one plant. Targeted not to have these 2 tent plants grow higher than 36” to avoid overcrowding.

I gave this beautiful girl LST and defoliation, let's see how she copes with the sters, She feels good

Salut les Growmies Nouvelle semaine de croissance , nouvelle coupe d'apex. Les filles vont bien et sont en pleine forme. le rempotage a accéléré leur croissance.Au regard de l evolution je pense les passer en floraison d ici deux semaines