All good

TRIPPLE G by ROYAL QUEEN SEEDS Week # 17 overall Week #16 Veg No issues this week she looking good looks like she is about to start to flower!! Stay Growing!! Royal Queen Seeds TRIPLE G

We made it to curing! I chopped her about 1.5 weeks ago, she dried well, and has been curing for a few days now. She could have went a few more days, but due to a PM outbreak in another tent, I wanted to get the whole grow room bleached for the next round, and wasn't taking any chances. We had a little bit of amber on non-leaf flower trichomes, so good enough! This plant was a beast. The second best producer out of the a lineup of 9 this round. That would likely be the Kush genetics in her, and to top it off, she smells wonderful. Sweet and floral. We haven't sampled any yet, and infact, I had to quit smoking, so we'll see what my father has to say in a couple weeks when done curing. After drying and partway through curing, we have approximately 19g of flower. We did a quick and rough trim with a machine trimmer, as we had 9 plants to take care of all at the same time, and didn't want them exposed to dry too long. I give this plant the highest rating out of any indoor plant I've grown over the 2 years I've done indoor, from a growing aspect. I'll post the smoke report when it is done cured, but for now, 10/10 -- would recommend!

Eh estado desconectado pero aquí va algo más de estas nenas, aun las tengo en periodo de vegetación. Lo que si esta semana logramos instalar la carpa correspondiente para estas nenas así que estamos esperando a llenar la carpa y poder pasar a floración. La estructura habla por si sola, aun no le hago amarres correspondientes ya que estoy esperando que me lleguen los soportes y poder darle más vigor del que ya tiene con el apoyo. Estamos probando cannaboost via folear 3 veces por día 2 ml x litro. lo vamos a utilizar hasta el día 21 de floración y comenzamos vía riego. quiero comprobar diferencias ya que todos mis cultivos tengo que probar alguna cosa nueva ya sea estilo de alimentación o lo que sea. Siempre dispuesto a resolver cualquier duda me pueden encontrar en instagram @chocolopeison Saludos

Vamos familia, hora de cosechar estas Biscotti de RoyalQueenSeeds. No veáis que pinta que tienen las flores están bien formadas y repletas de tricomas. Después de 70 días de floración obtengo estas flores tan increíbles que huelen pfff. Es una variedad un poco complicada de cultivar pero, que al final si da sus frutos. Bastante contento con este primer cultivo de la temporada de indoor. Dar las gracias a james por las genéticas dé RoyalQueenSeeds, y a todo el equipo de Agrobeta, que sin vosotros parte de estos proyectos no son posibles 🙏 Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Hasta aquí es todo , espero que lo disfrutéis, buenos humos 💨💨.

Wow...I wish I had of started 10 of these instead of 3,the one I didn't transplant is in full bloom and looking amazing, they look more like plants grown indoors more than outdoor grown. Candy kush express #2 is doing great but I'm wishing I had of put her in a 15 gallon bag rather than a cut up 10ish gallon bag but she's still doing wonderful. Candy kush express #3 is getting massive and almost looks like my other plants that are 1 or 2 months older!! I really pray that this bud is fire🔥🔥🔥🔥🔥🙏 🙏 🙏 feeding candy kush #1 raw,candy kush #2 is getting top feedings and foop organics and candy kush #3 is getting lotus

Looks like she’s going to be a yielder!

This plants a queen. So healthy and she just loves the training. I can’t believe how nice this plant looks and how well it responds to anything I throw at her. I will be taking some foliage off soon and topping one more time to get to 32 tops which has been my goal from the start 🔥🔥 😎🍻🍿

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Day in and exit fan 15 min off 15 min on Van is full on Night in and exit fan 30 min off 15 min on Van is off Lst tranings return Nutrients started 0.5gr/1L at day 21 400ML Per Pot Day 24 Grow powder 0.75gr/1L Short Flowering 0.5gr/1L 400ML Per Pot Day 26 Grow Powder 1.5gr/1L Short Flowering 1gr/1L 600ML Per Pot Day 28 Grow Powder 1.5gr/1L Short Flowering 1gr/1L 600ML Per Pot

For the Herbies contest - https://growdiaries.com/diaries/157348-grow-journal-by-dabbertglobberfield#gallery=6777378 - Grandmommy Purple is guilty multiple noise violations for being too loud and for stanky funk. Harvest - 169 days / 66 days in flower Well, we did it. I made it to the end. All I can think is...WOW that was A LOT of work. But I think I had very good results for a first time grower. I For harvest, we took Plant B and D and prepared them for immediate freezing. Altogether, I ended up with 1212g wet on plant B and 1089g wet on plant D. I ended up freezing 1816g of this. There was some from each of these plants that was set aside from freezing because, for whatever reason, I thought they were not worthy of washing because of how progressed the leaf damage was from the root rot battle. So that was cut up and dried as nuggets instead of hanging like the rest. Sawtooth and Plant C were both harvested and hung in the tent to dry for 12-13 days. I think I overdried by 1-2 days because when I jarred them they struggled to raise up to 55%. So I am using rh% packs. After drying Sawtooth and plant C, I ended up at 459.8g for dried flower and 170.8g in dried trim. So just over 1lbs between the 2 plants and 4.15lbs wet between the other 2 plants that was washed for hash. So... are you for real with the effort involved with washing bud for bubble hash? I don't think I would do this in the warmth. I only pursued doing bubble hash cause it's cold right now and I figured that would really help. Even in the 30s(fahrenheit) stuff would stick. It's hilarious how sticky of a process this is. I hope you have a bunch of gloves, water, and ice to burn through. I went through over 60 gallons of distilled water and 80lbs of ice to wash the 4 lbs. I am envious of people in videos using a hose to collect, clean, and refill. Also, doing this as a 1 person show is the definition of insanity. I broke the washings up into 3 parts. The first part was was the tester wash. I broke off 420g of plant D's 848gs available. I used 7 bags to see where I would end up. I used 220, 160, 120, 90, 73, 45, 25. What I determined was that if I was hunting a specific micron with a lot of material this would help find what you can get. But for the next washing I reduced the amount of bags to 4; 220, 160, 45, 25. The time, with the 4 bags, I washed with 809g. I had a much more favorable experience and return this second time but I had green fast and probably used too much ice. The 3rd wash was the remaining 631g and I found an extra 137g I added of sugar leaves and trim, brining the 3rd wash to 768g. I used 5 bags this time; 220, 160, 73, 45, 25. By the 3rd wash I have become an expert in collection but also never want to do it again. lol. 2 of the 3 washes have dried and I have 43.4g of bubble and 2.8g of dry sift. 37.4g of that bubble is able to be pressed into rosin. I will probably press that dry sift as well. The other stuff is 25u or 160u that I will experiment with or cook with. The other stuff is drying so I can't add it all in. I pressed a bunch of trim and some flower. The trim presses nice and the dab is decent. I am working out a good temp for the flower. But the problem with all these tests to find the sweet spot is that I have all these testers to go through but want to keep pressing and trying that! I think I will need to work on a better storage solution. I can't wait for the fresh frozen bubble hash rosin pressing... I have over 20gs of bubble to put in one bag. I am really looking forward to this. But I will need to work up to that pressing since I have never pressed hash/kief/bubble before in my rosin press. So this should be really fun. I will 100% post an update of the hash pressing once I do that and also after the 3rd wash session is done drying. In closing, saying I learned a lot from this grow would be an understatement. I fully expect to start it back up in a few weeks after some R&R then some R&D. There's some things I will need to work on before I start again, such as: having a single external reservoir that feeds multiple plants, hard to clog and easy to use ebb and flow system, miscellaneous final run stuff like space for drying. So thanks everyone for hanging out and perusing the photos and or reading all my huge rambling updates and thanks to those for the help along the way.

I'm happy with the way she's turning out.. bud sites everywhere! Starting to smell skunky, but nothing overwhelming!

Will be adding a whole lot of notes to the last couple weeks just trying to get all my harvest pictures up :) This one was a pure treat to grow . I am confident using exotic seeds that they can produce some if the world's best cannabis , hands down . They all were bushes , not too much stretch in flower at all . Usually in the first run with a strain I'm just figuring it all out.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

All I can say is WOW just WOW, One plant is hitting the light and I can't go any higher. It is 53 inches tall. All but 1 is flowering really nicely. The 1 is taking its sweet time. They seem very happy and healthy. I have to defoliate every other day or so.

There are images through an electronic microscope, I'm sorry, I can't keep a stable image, it's quite difficult, anyway, from what's there, how long do you professionals think...

Hey 👻 After many months without updates, here's a little one. These are the originals mother plants, i took 100's of clones out of em and then flipped to bloom 2 months ago. Here's a few pics of different bloom stages 👆 I began flush today after aprox. 60 days of bloom, i'm looking fwd to cut in 7-10 days, depending on trichomes 👍 I'm not expecting a big harvest but at least a quality one ! I kept a few phenos of Peach Tree, Pink Gasoline and L.A. Kush Cake 🙌 I'll post a final update when i'll harvest 😉

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