Bueno resumiendo cookies gelato es una variedad híbrida muy fácil de cultivar pero ojo, cuidado con los últimos riegos si no queréis perder cosecha por moho. Lo demás de 10 pegada heavy sabor increíble, cogollos duros como rocas y bañados de una gran capa de polen. El ambiente del secado se mantuvo en 23 grados de media y la humedad estuvo por debajo de los 45% en todo momento. Poco más la verdad estuve encantado de poder cultivar una genética tan potente . Un saludazo que paséis un final de año increíble y por supuesto buenos humazooos💨💨💨.

Now we are in week 8. Plants stopped growing. I will let them grow two more weeks and then I will at least harvest two of them. Depending on the trichomes I will keep the other two for 4-5 weeks more, because I will be away ... traveling lets hope that the sun is coming out again and that the buds get bigger and bigger in the last two weeks. BTW I stopped giving any type of nutrients, just water.

April 28, 2024 Day 8 Transplant day so first day of the veg/seedling stage. Roots were showing through the plugs and needed to be planted. They went into soil. Bio365 BIOFLOWER. It's supposed to be comparable to Sohum Living Soil which is what I normally use. We will see how it does I guess. 3 of the 4 babies are about 3 inches tall and the 4th is 2 inches. I'll have to keep an eye on that one. I don't want any runts that can't keep up. It will just get cannibalized. So I may end up with 3 instead of 4 plants. We will see how the next week goes. The environment is going to change slightly until I tweak it back into place. The water on the bottom of the tray will certainly get mostly absorbed by the containers, but there will still be enough to keep the temp and humidity in the area I want it. The light intensity also changed. Due to the height of the nursery bags, each plant gained about 4" in height. The light was left alone with the extension already in place. I can't get a good DLI measurement. I'll have to come up with something. Anyway, that's it for the day. Germination Center Environment: Temp: 78.7° RH: 82.2% VPD: 0.60 kPa April 29, 2024 All 4 seedlings are stretching nicely. The light seems to be on point for them their leaves have flattened out and look great. Good color too. They got a misting this morning. And will get another 250 ml tomorrow. Only plain water from here to mid veg if not early flower. We shall see how things go with them. They seem to be doing fine with the new soil. I'll keep an eye over the next few days to see how it goes. The plain water should be good. Although, I'm not sure what it means, but the soil is not a fertilizer. I wonder if that means I need to add nutrients at some point. We'll see how it goes. If all goes as it should, I won't need nutrients until week 4 at the earliest. The lighting is staying the same now. The plants seem to be quite happy with the intensity. In a couple days, I think I'll have to increase the light power to 90%. But not for a couple of days. The Germination Center hydrometer needs a battery, so I'm kind of blind with the environmental readings. The humidity seems fine as there is condensation on the walls. Temp seems alright as well. It should still be around 80°. Update: I increased the light power to 70% and dropped the heat mat temp down. The seedlings were all reaching a bit more than I like. They are already pretty stretchy, so I gotta control it. I also put in a different hydrometer for the time being. There's no VPD reading, so we will have to work without it for now. Also no daily average readings. That's a shame. Germination Center Environment: Temp: 80.3° RH: 84.2% VPD: unknown April 30, 2024 All 4 ladies look great and are reaching a bit, so I increased the light power to help prevent stretching too much. They are developing really fast though. I will most likely be planting them in their final containers in a week or less. The environment is pretty good with a temp of 78° and humidity at 83%. Still no VPD yet. But I don't really need it. Germination Center Environment: Temp 78° RH: 83% VPD: unknown May 1, 2024 All 4 ladies have started spreading their leaves and rapidly. They will definitely be out of the Germination Center in about a week. Hopefully a week and a half, but I highly doubt it. They are also the same height with the exception of that last one that wasn't really coming out of the soil easily. 🤔. I wonder if I should keep it. Let's see how things go in the next week. Maybe I'll be surprised. They also got 250 ml of water poured into the tray so the plants can suck it all up. The top soil is still moist, so no sense in top watering. I'm mostly using the tray to keep humidity up. It works wonders. I also shouldn't have to do anymore bottom tray watering for a few days. The lights are now set to 80% power and I think the seedlings are liking it. No more hard reaching, and the leaf growth. It just shows healthy plants. The environment is still great at 78° and a humidity of 84%. I plan on keeping it there until the plants get too big for the Germination Center, then I'll do my best to drop it so 70% for the first week of actual veg and then down to 60% for the second week on through week 4 of veg. Then I plan to drop it down to 55% and another 5% every week until harvest. Germination Center Environment: Temp: 78.3° RH: 84.5% VPD: unknown May 2, 2024 These Sour Diesel seedlings look phenomenal. The environment is fantastic as well. I had to increase the light to 100%. I want to see what happens. I don't think my Photone app is accurate for these lights. 60% power was showing a super high DLI of 36 mol/m²/d. The lights are only like 28 watts, so it should be at like 20 mol/m²/d at 100% power. One thing I did notice is that all 4 plants stopped stretching and are focusing on building up those first sets of leaves. It's like the perfect height from the light and they are as tall as the extension. The extension is 4" tall, so the seedlings are as well. The actual dome is 4" tall as well, so we have a good 4" of light distance to a 28 watt light set up. I wonder if AC Infinity has the intensity specs. I may send them an email. Maybe. Speaking of lighting, I can't speak for the intensity as my Photone app doesn't seem to work for this set up and I don't have a light distance to power reference. Or intensity reference. The environment is spot on with a temp of 78° and the humidity around 84%. Plenty of wiggle room for night time environmental changes. I added 250 ml of filtered water to the bottom of the tray again. The top soil is still pretty moist, so bottom it is. I'll probably do a top watering at the start of next week. That should coincide with the final transplant, so top watering will make the most sense and will be needed anyway to transplant the nursery bags. Germination Center Environment: Temp: 78.6° RH: 84.3% VPD: unknown May 3, 2024 Not much to do for these ladies today. They have plenty of water and I'm keeping the humidity at 84%. The temp is a bit high at 81° where it should be around 78°. I haven't quite figured out the lighting. The leaves are fine, everything looks good, but I honestly can't tell if they are stressed out or not. And if they are, is it the light or too much water or too rich of a soil? It could be any one of those factors or two maybe even all three. There is one who's edges are curling up slightly. I'm assuming it's the soil being a bit hot. But I'm not sure. Honestly, it's been almost 3 months since I saw seedlings and I have no idea what I'm looking at. Lol. I might be overthinking things, but there is some sort of stress on at least one of the plants. The smallest is looking good. It's just smaller than the rest. I'm still not sure what I want to do. As I said before, I'll wait for the transplant. I'd like to run all 4 plants, but I don't think it's worth running one that will most likely get cannibalized by the rest. Just a waste of soil and nutrients. But we will see. I'll do my best to keep it in though. Germination Center Environment: Temp: 80° RH: 81% VPD: 0.65 kPa May the 4th be with you. 2024 Star Wars all the way! Today is the last day of week 1 of veg. All 4 plants have excellent growth and are each working on their second node. I'm still hoping I can keep them in the Germination Center for as long as I can. However, I have 2 plants that are ready for harvest. So when they come out, I should be able to put the Germination Center in the big tent. Then there is only 2 plants left to harvest and I after I can do the last transplant into their 3 gallon containers. I have the opportunity to run 5 gallons still. I have 3 yards of soil. I think it's enough for 4 plants. Definitely enough for 3. If I have to get rid of this smaller one, I'll definitely be running 5 gallon containers. The lights are perfect at 80% power. I can only assume the DLI is around 14 mol/m²/d to 16 mol/m²/d. The plants are looking great with it. Just a slight reach and wonderful color. The environment is quite nice indeed. The temp is hanging around 77° with the humidity still at 84%. I'll be dropping the humidity down to 75% starting next week. It'll drop down to 65% to 70% when the transplant happens. Germination Center Environment: Temp: 78.8° RH: 82.7% VPD: 0.58 kPa

Day 78🌱🙂🍊 This lady is doing her great job, Buda are increasing rapidly 🌱 difference between top flower and bottom is evident. For this reason I’m quite sure I’m going to cut in two times, around 85/87 days I’ll cut the high part of biggest bud and around day 95 the rest of the plant. Day 80🌱🌱🌱🙂🍊 I’m going to go up to 90/95 days, smell now is really strong 👃🌱🤣 I keep giving some water when is really dry because some parts of the plant still needs it. Day 81 🌱🌱🌱🌱🙂🍊 Hi all fellows, today a big defoliation cleaning to improve light in the bottom Part of the plant before harvest. I tought to harvest in like 10 days but as I have some water yesterday I noticed that she’s been quite not drinking anymore. I’ll give another 2/3 days before harvest. I will be perfectly in 85 days from seeding. Smell is crazy now, I can smell cotton, orange, chocolate, pinus.. can’t wait to try it. Let’s see! ANY SUGGESTION IS REALLY WELCOME 🌱🌱🌱🌱🌱🌱🌱🏆🏆🏆🏆😊😊😊😊

So she had some nice growth this last week. I'm hoping this next week is its last bit of growth, can only move the light up another 3 to 4 inchs. I've got to clean up the lower section A bit more, more growth there then I'd like. So far it's growing a lot nicer then the last plant I did in this tent with a SCRog set up. The size of the squares on the last grows netting were 6" and this one is 4". Way easier to get a proper looking SCRog plant then with the 6" that's for sure. Now that the temperature outside has warmed up the night time temperature has been better then earlier weeks. The humidity has also gotten a lot better. Especially after I add a 4" muffin fan to the lower 4" duct hole. The humidity went up but the temperature went down a bit during lights on. Ive had to lower my temperature switch just to get the 4" duct fan to turn on to suck out the stail air. Think what I'll do is add a second plug to the temperature switch so I can add the 4" muffin fan to it so that itll only turn on when the temperature goes up instead of running 24/7. I've increased the feed up to 1000ppm and am give it 1.5L of water a day with 250ml max run off. My run off ppm is only 1080ppm so that's a great sign so far. I've been trying my best to keep the ppm levels equal every feed but itf luctuates a bit but no more then 60-80ppm. Same with my Ph, because I'm using the Jungle Juice base nutrients it's not part of Advanced Nutrients Ph perfect line so I have to adjust it. I usually try to have it swing back and forth between 5.9 and 6.1 and the plant seems to be liking it. Tho I'm not done this grow yet this light is doing a lot better in this tent then the last light. Only downside is that one burnt out diode. Because I always try to plan a grow ahead, I'm already planning on trying that last light out again (Phlizon PH-1000 125w that they apparently don't make anymore). But this time it's going to be in my 2x3x5 fusion hut tent with Royal Queen Seeds Fast Eddy CBD AUTO strain. Also going to add a ex hale bag to it. Well that's all for now growmies. Keep growing!!!

The beginning of Week 6 of Flower and boy is she getting stinky. I have high hopes for this plant to live up to her name .

Starting 12:12 at Christmas with my ViparSpect XS3000 @80% (240W) + new 2 additional SpiderFarmer GlowR80 (80W) that turn on 10min before, and off 10min later than the main light

this is the beginning of the 4th week along with the publication of the end of the 3rd week, this is how the fourth week is received, another intensive pruning that has no choice but to do it due to the size of the indoor, which is working great for us and we believe that I will always work like this, I am happy, here then the pruning of its results a little before and after and how it is receiving this fourth week, with the threads I touch a little bit of hair I hope it does not affect much but all in favor of opening the field to all the branch buds alike.WE CHANGE THE MESH FOR A BETTER ONE AND OF THE IDEAL SIZE AND AGAIN WE USE THREADS THAT HELP US VERY WELL, WONDERFULLY

* harvest: day 134 total. Day 64 of flower. 19 weeks total * dry bud yield: 128gram * Appearance: gorgeous blue/green/purple'ish buds that shine like a diamond * Bud density: 9/10 * Aroma : when you open the jar a gas smell mixed with orange bubbelgum hits you in the face * Taste : orange zest bubbelgum with a strong gas taste * Potency : 8/10 * Kind of high: a nice sativa dominant feeling. With a lot off medicinal effects. It takes your pain away asap and it relaxes the musscles. Also makes you feel happy. * Colour of thrichs at time of harvest: 30% amber 70% milky * Drying time: 10 days in the dry tent, 12 days in the jar * Length of buzz: 2 -3 hours * Good comments: the most beautifull plant i ever grew, really impressed with these genetics. Such a good pain killer and anti depressant! The buds where almost pure oil when i was trimming so i would really recommend it for pressing or hash making. Wil grow this again for sure, its by far the best thing i ever grew

Day 25 Second topping 🤒🤕

Lowered bloom nutrient to 16 mL per gallon and hydroplex to 4 mL per gallon. EC now 2.3

The calm before the storm in the Scroggernaut - either yer scroggin or yer not ACMPR licence holder, allowed to grow up to 49 6 mothers, 2 strains for the 2020 photo run. Pheno hunt the best veggers to pick my 2 best moms, give away the rest. Also have 4 autos in there to kill some time while I veg out the moms Running 16 plants total over 40sqft ( a 4x8 and a 2x4). Will be doing 12 x dutch buckets coco/perlite mix on an automated feed and drain to waaste, as well as 4 x soiless mix handwatered for my son to learn the basics of growing. Big ups to the BOOFPARTY crew Lights are awesome. 3 x HLG 550 clones LM301b diodes. Room is self contained, fully climatised and insulated with vapor barrier. This is the garden I have always hoped to build Will be mostly video updates here, limited data. Not looking to win any contests with this

- Week 6 - ----- Day 36 ----- No feeding today as it was tomorrow so nutrient line up isn't up yet. I'll do that tomorrow. Ladies are perfect, I can't wait. less than 30 days away. ----- Day 37 ----- Today is feeding but back left is behind the other 3 again, gave the other 3 500ml of tap water (10ppm) to prevent turgor pressure loss while back left dries up. Should be feeding in 3-4 hours. 23L 1100-1500ppm (new feeding, so ppm end is not known atm) 6.4 pH Plants are insane, big Bertha has become middle Bertha as the other stems have fattened up quickly. Speaking of which plants are in bulk period, 3~ weeks left so it's an exciting time to watch them fatten up. **UPDATE** Fed at 1000ppm 23L 6.4pH The removal of FloraNova Bloom, and letting the chelated Diamond Nectar go to work on the molasses brings down the "accountable" parts by the machine I use. 1000ppm is full strength on all fertilizers being used. I might add more molassses in the future and bring it up to 2ml/L, and maybe a smidge of epsom salts next feeding via foliar or direct feeding after I do some research. No more KoolBloom liquid, KoolBloom powder begins and she's a strong one. ----- Day 38 ----- Yesterday back left plant has me unnecessarily anxious. It didn't need watering till 2 hours before lights off. What I did is fed the other 3 90% of the feeding, and only gave back left 10% (closed the irrigation heads till the end of feeding). I'm HOPING it was the plant transitioning from pre-bulk flower to bulk-flower stage, and maybe it slowed down photosynthesis to concentrate on hormonal changes. I dont know I'm anxious and just talkin out loud. I'll know within 72 hours if there's a major issue with back left. Plant looks fine, i'm just paranoid. Pictures later today around mid light cycle. Buds are getting insanely fat. **Update** Was bored. Here's some wicked shots. Still 25-27~ days left and its big bulk time. Can't wait to see them at the end. ----- Day 39 ----- Everything is great. Feeding late tonight or potentially tomorrow depending on back left plant. I want to make sure bottom center of the root zone has dried out and doesn't begin to mold before I water again. This will also help prevent fungus gnats in the soil. I'll keep the other 3 away from turgor pressure loss, but they got 90% of the feeding last time so I expect them to dry by the time back left is. *** Update *** Now the other 3 plants who were faster on water intake than back left yesterday have slowed down. Weed growing knowledge at a Horticultural greenhouse scientific repeatable level is pure fucking garbage. I have 6~ books and checked dozens of sites and there was major conflicting info on water intake. That's the problem with cannabis, it was illegal for so damn long horticulture practices and knowledge never molded or were used in the process and now it's anyone's fucking guess as to who is telling truth or is spouting bullshit bro-science. Don't even get me started on names. What a stupid god damn naming system "counter-culture" created... I'm growing wedding cake.. WEDDING CAKE? Wtf. That's stupid as shit. Give weed botanical Latin names based on appearance and technical factors PLEASE!!!!!! If I could name this plant it would be (Cannibas v. hybrid 'multa-magnum-fragum-gemmae-dulcis') why? It's due to it being a; Cannabis plant, hybrid variety 'multiple big bud strawberry candy' showing off multiple long bud stems smelling of candy, Is that more convoluted and arrogant than Wedding cake? YES, it is. Is it better? YES fuck your stupid wedding cake name. That's dumb. And you should feel bad. Still want to argue? Name it Cannabis v. Hybrid 'crustulam nuptias' THERE! Wedding cake. fuuuck! 99.99% of plant names are Latin. Anyways... back to the point... I hypothesize that there is a secondary transition portion of flower where the plant (like in stretch transition) pauses its growth to move the hormone and nutrient production to other sources. This secondary pause happens JUST before bulk session begins. This hypothesis states roughly that up until final big bulk period (last 3-4 weeks), fan leaves and sugar leaves could still be produced by the plant during a "predator" or "high weather event" situation that would cripple the plants photosynthesis for final bud/seeding production during early to mid stage flowering. Once the plant has reached adequate nutrient reserves or some other time or light cycle based trigger, it removes the hormonal/internal production/ability of sugar/fan leaves still being potentially produced out of the internode stem cells and fully stops that ability and concentrates 100% of growth on bud structure and protection of bud structures through THC/CBA/CBC/CBD etc. (Im a little tipsy, can't spell cannabinoid leave me alone...) production. Tonight I will at 30 minutes before lights off be judging the water requirements during sleep of each plant to prevent turgor pressure loss, and to allow each plant to finish this transition and get back to major water intake by feeding tomorrow morning. It's insanely obvious somethings changed. I Was worried that leaving back left plant with stagnant water in the bottom saucer (pot doesn't sit directly in the water but is 3 inches above it) had released mold spores and started destroying the root mass of the back left plant but: 1) No obvious plant death signs, weakness or other aspects of it being a slow death from bottom up. 2) The other plants did the same damn thing within 48 hours.... and the back left plant is by far the largest and gets the most "light" due to its size hence it would most likely (HYPOTHESIS) finish its secondary cycle a bit faster due to just volume and weight. Listen i'm not gonna say this is all 100% correct knowledge, It's based off feeling and "knowing" plant cycles as a greenhouse certified Horticultural Technician. Repeated efforts to grow and replicating situations that created these things over and over is the real proof and will come in time. One major benefit is the fact I WILL be re-flowering these for the VERY LEAST 3 full flowering cycles as long as they don't die during flower to veg transition. I will be getting them THC tested EVERY SINGLE flower cycle to prove without a doubt the "maximum maturity" possible of a weed plant re-veg flower cycling, just like how we know by the 5th or 6th successive clone, the DNA will deteriorate and the grow will be stunted or a lesser bud high and yield. God I fucking ramble when I'm bored and tipsy. ----- Day 40 ----- Fed at 1350ppm 6.3pH 23L Plants are doing great. **Update** Little fun video from mid light cycle. Roughly 20-25 days left. Will finish at 30% amber 70% cloudy and will cut branch by branch as they are done, not in a rush to do 1 single cut and will let them survive to re-veg easier and less stressful. ----- Day 41 ----- Easy day. Here's some pics. Just basically have to watch the grass grow today. ----- Day 42 ----- Big photo day, used ruler for most shots. End of beginning of bulk, big bulk starts tomorrow. Could probably feed late night tonight but I'm going to just give 500-750 ml of water depending on roots needs before lights off. Tomorrow will be a big feed for first day of week 7. Tropicanna Glookies tips on back right are beginning to darken and become purple. I am hoping this whole plant turns purple in last 2-3 weeks. We will see. **Update** Back right plant is starting to turn purple and FAST. Pretty awesome.

Dump de lo que fue esta semana. Seguimos ajustando con lst la estructura que quiero y empezamos a la transición a flora. Esta semana será la última de vegetativo donde trataremos de sacar clones (esquejes) de ambas. Ya que he estado mirando videos y tomando información de cómo hacerlo, creo tener una idea de cómo hacerlo sin fallas en el intento. La próxima semana nos veremos realizando estos esquejes y veremos el avance de esta última semana. Recuerden que todo lo estoy haciendo de forma empírica y sin conocimiento previo. Todo ha sido con apoyo a videos de otros growers e información de distintas páginas. Gracias por estar aquí y compartir conmigo esta primera experiencia!

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

OK, im doing an update now becouse my breakup cake is going tranny!!(hermie) I already have noiticed she was not growing as strong .. but u can blame that on anything , especially when growing outdoor.. but this morning i saw that she was g(r)oing 'bananas'!😢 I have put her aside from the rest (in the back of garden) but think gonna have to terminate her asap!..(and i will, right after this post👍) The weed she will give will be filled with seeds and those seeds will be hermies too, so wothless.. and if i dont throw her away quick she can infect the rest. So goodbye breakup cake..😢 who wants to eat a cake when they are breaking up anyway..😒 (just hiding my pain with lame humour 😳) happy growing for all ✊