week 13. I checked the trichomes and im going to give them another week. My carbon filter is working Overtime.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

The final phase... Lot of wrong and lot of broken... All job is is gorgeous.. end I love teach by my wrong Everybody see my diaries I love you tell me a little comment about... I like received teach to experience people...... By my now grow call purple lush and ayawascha purple barney's seeds... My Las is only a 205watt effective... I wrong to not buy another 200w... È and the harvest is more little than 400w... But after in 2 time after I want yield 100gram?.... Whats you think about the max yield?

Hi liebe Community and Welcome Back! 💚 Woche 9 (Tag 66) im Vergleichsgrow! Bio Tabs NL. vs. Weedefix (Stuttgart/ Germany) Hier im Bio Tabs Grow haben wir nun nach 66 Tagen des Einpflanzens eine Höhe von 136cm erreicht. Der Stretch geht nur bei der Bio Tabs Pflanze so steil. 🤷 Blütetag: 21 (BW3) Nach der dritten Blütewoche, lässt sich eine etwas differenzierte Performance der beiden Düngersysteme beobachten. Der Stretch performed bei der Bio Tabs NL Pflanze etwas krasser. Einer der Triebe ist nun krasse 136/137 cm hoch, was ich allerdings persönlich nicht so schätze, da die Höhe auch immer für mein Zelt ab dieser Größe gewisse Herausforderungen mit sich bringt. Es bleibt spannend! Diese Woche, habe ich einmal mit Wasser und 5ml Bio PK 5-8 (Ferment mit Melasse und Bananen extrakt) gegossen zudem wie angegeben ab Blütewoche 3 nochmal 1g Bactrex hinzugefügt. Ansonsten nur Wasser. Die Bedingungen im Growzelt sind aktuell wieder in Ordnung! ————— 🌞 Temp: 26 🌚 Temp: 18°C bis 19°C 💨 RH: 62% VPD: 0,74 kPa 💡ppfd: 630 mpm —————

Starting to see nitrogen toxicity in the Solomatic, I'm watering with only PH water since 1 week now

Lilly x White Widow is doing well understeer Spider Farmer G5000/UVR40 lights. She is the smallest plant in the group. She is stacking nicely, and forming some frost on her. She has had a strong appetite. 🤞🏻I can keep up with her till the finish. Nothing else to report at this time. Thank you Ripper Seeds, Spider Farmer, and Athena nutrition. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g Spider Farmer Official Website Links: US&Worldwide: https://www.spider-farmer.com CA: https://spiderfarmer.ca UK: https://spiderfarmer.co.uk EU: https://spiderfarmer.eu AU: https://spiderfarmer.com.au G5000 Light Amazon Link: amzn.to/4643esa UVR 40: https://www.amazon.com/dp/B0BR7SGTHS Discount code: saveurcash (Stackable)

Day 22. Last 3 girls got final house. 20 liters pots for those late runners. They look sad, but maybe big house and other girls will encourage them to kick in. I took last NL away and now humidity and lightning are a lot better. Will drop lights down in few days, but not a lot ... 16 girls again in one room ;)))) I changed names of diaries, because I will try to finish them indoors ... Happy Growing !!!

lil Zstraw is filling out nicely and flying right along , same same next week . I’m so happy I held on to her

11 Noviembre: Primer riego con 2.5EC y 5.9pH, runoff de 2.9EC y 6.2pH, en una semana más ya deberían estar recibiendo 3.0EC y seguir con esa concentración por todo el cultivo. Alturas están entre 15cm y 20cm. 13 Noviembre: Riego 2.5EC y 5.9pH, runoff de 3.3EC y 6.2pH. Hoy se removieron los primeros nodos de cada planta y se les hizo poda apical. 14 Noviembre: Riego 2.5EC y 5.9pH, runoff de 3.3EC y 6.2pH en la mañana. En la tarde se realizó transplante a maceteros definitivos de 5 litros con 4 litros de sustrato aprox. el cual fue pre cargado hasta la capacidad de campo, con 3.0EC y 5.8pH, desde este punto se debe esperar un par de días hasta que el dryback(secado) del sustrato sea de un 35-40%, lo cual vendría siendo el primer P3 del cultivo, una vez se alcance ese porcentaje de secado, se comenzará con los primeros riegos de precisión llamados P1.

Giorno 6 annaffiata con 2,5L di acqua Giorno 7 defogliazione

16.2.25: I decided to check out the lower canopies of all plants to see if I need to get rid of any foliage. I did get rid of a few small branches and leaves. However, whilst I was doing this, I saw more garden pegs from my LST remaining. There were about 6 or more in Pink Mist alone. Additionally, on Watermelon, there were some left in, too. I'm so annoyed to see that because the plants are really stretching, and I could've potentially disrupted this by leaving the pegs in for all this time without realising it. 🤞 that I haven't compromised things too much. We'll see. I watered today with 2ltrs of dechlorinated water PH'd to 6.3 containing the following nutrients; ♡ .8g Green Leaf Nutrients PK booster ♡ .5g Ecothrive Biosys I ordered quite a few things for the garden. I got Greenleaf Nutrients Sea K(elp) and Mega Crop Parts A+B. To go with their PK Booster I got last month. I'm excited to try it all together. Next run, maybe just using these. We'll see how it goes. 18.2.25: The plants are going crazy for water! Everything is getting used right up so fast! Today, I decided to add some more Black Strap Molasses to add some carbs and other micronutrients. I'll add the jar with the label in the photos section above. I watered a very small amount to each plant. What I put in: ♡ Black strap molasses 150g ♡ 2g Sea K(elp) Greenleaf nutrients. I dissolved everything in 4ltrs of dechlorinated water PH'd to 6.4. 19.2.25: I received the majority of the garden purchases that I made. I'm still waiting for the Ecothrive Life Cycle. I wanted to top dress, but it's been delayed unfortunately. I am using my Greenleaf nutrients products which I bought on Amazon. I got the Mega Crop 2 part system Part A and Part B. I have the Sea K(elp), and the bud explosion PK booster. I really wanted to get some of their sweet candy asking read many positive reviews. Unfortunately, for me, this is unavailable to buy currently. So that's a little disappointing. I needed to do a good watering so when my nutes were delivered today, I got excited 🤗 I watered 2ltrs of dechlorinated water per plant, PH'd to 6.4, containing the following nutrients: ♡ 1g Mega Crop Part A ♡ 1g Mega Crop Part B ♡ .5g Sea K(elp). The plants drank this up within a few hours. I'm going to try and hold off on watering in hopes that my Ecothrive Life Cycle will arrive so I can top dress and water it in then. 20.2.25: My Ecothrive Life Cycle arrived yesterday, and the plants are ready for their top dress and a good watering in. I have some Biobizz Light Mix, Canna Coco,and perlite. I'm going to use this as a base to mix my amendments in. I'm going to fill my 5 gallon bucket with about 4.5 gallons of my top dress mix. I will distribute this across 6, 4-5 gallon pots. Then I will water in well with Greenleaf nutrients Mega Crop Parts A+B and Sea K(elp). I've made a crude attempt to video mixing my top dress. Don't listen to the audio. lol, my YouTube didn't stop playing whilst I recorded this 😂 So anyway, I added the following amendments to the above base mix of 4.5 gallons; ♡ 3 TBSP Ecothrive Life Cycle ♡ 3 TBSP Vitalink Bat Guano ♡ 3 TBSP Ecothrive Charge ♡ 1 TBSP RHS Mycorrhizal Fungi granules ♡ 6 TBSP Ground Cinnamon.

Watch the video guys, better info on there 👍🏾..................................................................................................................................................................................................!

📆 Semana 5: La Blue Zushi está entrando en su mejor momento. Los cogollos empiezan a formar unas colas gordas y compactas, y el aroma ya se vuelve más complejo: mezcla de frutas dulces y un punto funky muy interesante. Los Adlite de Mars Hydro están sacándole todo el potencial, con una luz muy homogénea que hace que incluso las puntas más alejadas estén produciendo resina. Se nota cómo mejora la estructura y la densidad de las flores bajo estos paneles, ¡una pasada! La nutrición sigue de la mano de XpertNutrients, y el resultado salta a la vista: hojas bien verdes, tallos fuertes y sin signos de bloqueo o deficiencias. La planta se mantiene sana y empuja con fuerza hacia el final. Ahora toca seguir mimándola para que termine de explotar en las próximas semanas.

12/16/2021 Recovery is a success! A little raggedy but putting on decent growth in last couple day's. Also need to decide if I top or mainline the two tops? Going to feed her today and see how she grows this week then decide what action is appropriate. She actually has 4 tops! Three are more developed but if you look closely there is 4. I kind of want to let it grow, although it may cause a split later on😕 . So still thinking on it.

Harvested choc 5, not a great washer based off what I got from the trim and larf, all the big buds are drying, took a whole rack on the vcure.

Last week went well. She is growing steadily and I can't wait to get her outside. She is going to be great once she can spread her roots in the ground. I took a few clones to attempt to keep this strain around since it was my only seed. Can't wait for the fall. She had some leaves removed and I did some lst and supercropping to even out the canopy further. She was topped on every branch to double the amount of tops. I will update as she progresses. Until next time happy growing to everyone!

We are in the 16th day and these babies are developing very quickly.

Close to the end of week 5 of for the two on the left. Starting to fatten up and fill the top 10 inches of most stalks. Pretty happy so far. The girl on the far right is just starting to form resin glands, hopefully she packs em in because she's on track to have some nice nugs. Looks like she's a little darker green this week. I should just cut her out of the rdwc and fix her. Day 77 moved the three girls to a bigger tent with a couple other autos. Decided to keep them all in rdwc vs dwc because i'm lazy. I did clean everything pretty good though

Looking good!

We continue to mix our own nutes, but I started also testing Nutra Hydro . One rack only for now. Next project is upcoming for organic beds or huge pots. STAT TUNED BROTHERS ANS SISTERS ! Grower love ❤️❤️❤️!!!!