N deficiency but it’s ok.

Beginning of the 10th week of flower. Shouldn’t be too much longer now. I will add some bud and trich pictures this week.

July 5 - The girls are still doing awesome. temps are between 21-26. Humidity is 40-50. Still feeding every 24-36 hours with 3L per plant. July 6 - They are doing good. Showing lots of flower sites now. Unfortunately I did not get any pictures today. July 7 - Tons of progress since last week. I don't see any signs of any problems, and still no pests! The smell is getting A lot stronger as it starts to flower more July 8 - Still doing there thing, no problems, temps and humidity is fine. Have been feeding every 24 hours now as they seem to be really thirsty July 9 - Super happy with how they are doing. No issues yet. Smell is heavier, and tons of bud sites showing up. Only issue is they are growing pretty uneven. July 10 - Time to get a even canopy! First time doing some HST. I also did some defoliation. One of the stems split open a bit but it seems to be doing just fine. I did he HST and defoliation in the morning, i then took pictures of 12 hours later and everything seems just fine! July 11 - Everything seems to be a lot more even and the broken stem doesn't seem to affect the growth.

Nematodes inoculated. Topped, I'll probably start quad-lining it soon. Clone started in soil (mulched with its own chopped leaves). The cover crops are invading this little garden. It doesn't show but it has already been trimmed last week. Radishes will be harvested in about 10 days, it'll make some more room for basil that I'm propagating for this tent. [Done] I'll be applying a seeds sprouts tea this week end or early next week (I haven't soaked the seeds yet 😅). [Done] Place training frame for LST on the quadline Side note: this young plant is slowly starting to smell like potential goodeness 😉

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Flowering day 1 since time change to 12/12 h. Hi guys :-) . The time has finally come 👍 You have been switched to 12/12 hrs and are ready to flower. Of course, cuttings were cut beforehand to keep the genetics. For 4 days now she has also been getting Fast Buds from GreenBuzzLiquids. They were also sprayed again with GBL Fast Plant spray. She is very healthy and I am excited to see how she gets going again in bloom :-). This week it was poured twice with 1.2 l each time (for nutrients, see nutrient table above). Then I filled canisters with fresh osmosis water for the next few weeks 👍. The tent was cleaned and every plant was checked for health. At the moment I am tinkering with the ventilation because there are currently 4 fans with 4 activated carbon filters running, which I want to make more efficient by putting a hose through 2 tents. These are the things about which you desperately at the beginning but learned from them in the end. I wish you all a nice week. Stay healthy 🙏🏻 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. You can buy This Strain at : www.Zamnesia.com ☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼 Strain Gelato clone from mother (Zamnesia ) ☝️ Genetics: Wedding Cake x Gelato x Gelato 33 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Nutrients : Green Buzz Liquids : Organic Grow Liquid Organic Bloom Liquid Organic more PK More Roots Fast Buds Humic Acid Plus Growzyme Big Fruits Clean Fruits Cal / Mag Organic Ph - Pulver ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8

3/23/25 - Today marks the start of week 2 of flower. I top-dressed the soil with Gaia Green 2-8-4 at 2 tablespoons per gallon of soil, Gaia Green 4-4-4 at 1 tablespoon per gallon, and worm castings at ½ cup per gallon. I watered with 1 liter of water, pH’d to 6.6, mixed with 1 tablespoon of fermented plant juice per gallon. Light is at 740 ppfd.Everything is looking good as the plants continue to transition into flower 3/25/25 - added water to the reservoirs today. PH'd to 6.5 3/29/25 - Filled the reservoirs because they were bone dry. Plain water PH'd to 6.2

Week 21: August 16 2020 - August 22 2020 Great week, weather-wise except for some strong winds in the middle of the week. Some branches and leaves took some damage and went a little limp, but they all seem to have bounced back. Still more yellowing, I'm pulling dead leaves off the plants pretty much every day. I know it's natural and it has been a bit cooler at night recently, but I'm still a little bit concerned. Other than that, new growth is coming in strong, has a nice green color, and the little buds look healthy so far. First full week of flower under the belt. Thanks for stopping by. Cheers!

Last week flush for second week and than 2-3days of dark cut, hang up,and rest for week

💩Holy Crap Growmies We Are Back💩 Well growmies we are almost there , she got flushed so her days are numbered💀💀💀 👉 Shes a short chunky little plant,😉 Lights being readjusted and chart updated .........👍 Even with early major issues due to the soil/medium she's come a long way 👈 👉I used NutriNPK for nutrients for my grows and welcome anyone to give them a try .👈 👉 www.nutrinpk.com 👈 NutriNPK Cal MAG 14-0-14 NutriNPK Grow 28-14-14 NutriNPK Bloom 8-20-30 NutriNPK Bloom Booster 0-52-34 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉THANKS FOR TAKING THE TIME TO GO OVER MY DIARIES 👈

8/17 Watered everything but the 50. Found two leaves with sep markings on the pink kish in the 50. Definitely not using old soil again. I'm pretty sure THIS plant contracted it from the soil and not the way the others did with the lawn mower and birdseed hijinx. It's supposed to rain for the first time basically all summer. It's am 80% chance so I HELD OFF ON PLANT DOCTOR AND DID NOT APPLY as I'm supposed to have a clear day after. I'll apply the plant doctor in the morning. I'm hoping this rain will knock down a bunch of those thrips. They seem to be on a small branch on a plant or two. One or two leaves show damage and I'll pick them off. I figure I'll get them after I apply plant doctor. I'll use either citric acid or just my regular bt-k pillar treatment with Castille or liquid soap. That will kill them as well. I'm on really worried about it. I also might just buy a bunch of lady bugs and unleash them once things get further along. EDIT: TOOK A QUICK VIDEO AND A COUPLE PICS. HOPEFULLY WE GET THIS RAIN. IF NOT IM GOING TO TREAT THE THRIPS THAT ARE ON TWO PLANTS NOW. I THINK IM GOING TO GO WITH BT-K FOR NY PILLARS AND HOPE THAT THE DISH SOAP IN THE MIX KILLS THE THRIPS. I HAVE LOTS OF DIFFERENT OPTIONS SO ILL FIGURE SOMETHING OUT. BUDS SEEM TO BE EXPLODING IN GROWTH. OH AND THANKS TO THE OUTDOOR GROWER THAT MESSAGED OFFERING TO HELP. I APPRECIATE THAT. THANKS. 8/18 We got the rain we were expecting. It was sheet rain for a few hourscand rained during the night. Everything was drenched and it was cold (50°F). I shook off the special kush that's way further in flower. I decided to use the leafblowrr despite the risk of spreading anything. I did it so that it wasn't ever blowing TOWARDS another plant but still. I was hoping it might blast off some thrips that might have survived that torrential rain. We've never had a dry summer like this. I'll moniter things. My water day is tomorrow so I assume that's when the girls will get their plant doctor dose. It's a great time of year for cannabis growers. We get to watch all the hard work we've put in literally pay off. It's very peaceful in the garden. EDIT: Went over at about one to check things out and do some minor defoliation. I checked on the thrip situation and I dont know if I blasted them off with the leafblower or if the rain washed them away bit I doubt it. Ivecmade the decision to treat these little bastards. It's on one plant but it's started to spread to another branch on another plant. I probably just overlooked it but still. Seeing that it rained like he'll and I'm seeing like zero signs of septoria I'm going to treat everything. At least I think I am. I haven't decided what to use. I think I could just use bt and soap and I'd probably be alright. I'd feel better doing that as it's something I'm familiar with. Outdoor growing. It's always something. 8/19 In the 40's last night. Hopefully that will help with the thrips. I was hurried and since today tops at 72° I only watered the 10th planet big mk ultra and the chem dog with preventative plant doctor. I'm not seeing hardly any septoria and if I do itsca random leaf. I mixed up 3tsp of citric acid and some Dawn in a 2 quart hand sprayer and treated the 10th planet that I thought had heat stress and the Pink kush in the ten gal that has the damage. I also treated a branch on my good tenth planet. It was the only branch with markings so I think I've got it early enough. I'm just wondering the best way to tackle this. The pink kush I'm sure, could handle spinosad. That strain is much later flowering. I'll see how the citriccacid works and go from there. If the other girls need water they'll get it when I get home alongcwith plant doctor. I've got some work to do. I'll keep this updated. Opinions are always welcome. UPDATE: THE PLANT IN THE TEN I SPRAYED LOOKS BETTER THAN WHEN I SPRAYED IT! I ALSO DIDNT WATER AS ITS UNDER 70° AND EVERYTHING I WATERED LOOKS WORSE THAN WHAT I DIDNT. WATERING NEEDS CHANGE QUICK WITH A 40° TEMP SWING. THESE THRIPS ARE GONNA HAVE A FEW MORE NOGHTS IN THE 40'S. THE CITRIC ACID SEENED TO KILL ON CONTACT. MY PROFESSIONAL BUDDY ADVISED TO ORDER GREEN LACEWINGS AS OPPOSED TO USING SPRAYS. HE SUGGESTED SPOT TREATMENT BUT AFTER MY RESEARCH ON THRIPS THAT WONT HE AS EFFECTIVE. I HAVENT DECIDED WHAT TO DO. I MAY ORDER THE LACEWINGS AND I MAY CONTINUE AND ROTATE TREATMENT. I DONT WANT TO OVERREACT (WHICHVI TEND TO DO) BUT I ALSO DONT WANT ALL MY LEAVES TO DIE. ILL KEEP THIS UPDATED. IM PLANNING TO GO OVER TONIGHT AND CHECK THINGS OUT. I MAY SPRAY A FEW MORE PLANTS AND WATER WHILE IM THERE. ITS TIME FOR THERE PLANT DOCTOR PREVENTATIVE. 8/20 I watered the two kush in the back WITH THE PREVENTATIVE DOSE OF PLANT DOCTOR as they were dry I assume due to the wind they get. The one in the 50 I found one sep leaf. Looking carefully arpund the garden I can tell that I have a thrip infestation. First time I've fought this and it seems most people don't really know what to do besides predators at the beginning of the season. I'm too far along for spinosad. At least thats what I've been told. My pro buddy said to try to spot treat it. It's not bad bit I'm afraid it might be worse than I think. The plants I treated with citric acid look MUCH better. I think I'll dial the dosage of citric acid down and start with that tonight and switch up treatments. I can get rid of them it's just going to take a ton of work. I think I've had them before and I attributed to something else. I so think that the very early pink kush plants could handle spinosad. I hope so. The plants I watered the other day look overwatered. The plants I didn't water look GREAT. The special kush in late flower looks happier than I've ever seen her. The difference being we went from 104° one day to 72 the next with 46° night Temps. This week is highs of 70's lows of high 40°'s. I've got a lot of work cut out for me. Didn't do a video as I didn't have time but I'll do one. EDIT: Nothing needed to be watered at noontime and I'm trying to not overwater so I left them. The two I watered this morning are fine. I'm glad I caught this thrip bullshit when I did bit it sounds like it's going to be a HUUUGE pain in the ass to beat. One thing right after another. I've found those suction marks and the silvery shit they leave behind on pretty much every plant now. I'm thinking me using the leafblower (away from the other plants I THOUGHT) must have blown a bunch of them around. I'm not seeing a lot of bugs. Mainly feces. I did find a couple adults. Honestly, I've probably had these before and not known it or attributed it to something else. Literature I'd all about spinosad which of course I have on hand. AI told me I could use spinosad in early flower so MAYBE I could treat the pink kush plants with it but ill have to figure something else out for the other girls. I'm planning to treat tonight depending on where the research takes me. Not sure exactly what I'll be using but I'll keep this updated. 8/21 IT got cold last night. It was low 40's when I went to the garden. I watered the special kush in late flower and the mk ultra I kept natural. Did some defoliation. Found little feather on a large branch. Birds have been helping with pillars. I left out the plant doctor on those watering as septoria has pretty much been eradicated. Plants are drinking significantly less water. It went from 104° day time to 72°. Same with the nighttime Temps. Luckily with Temps like this the thrips don't seem to have spread. I found a couple lacewi g eggs while looking for pillars. Hopfully they will help. Maybe spot treatment and decoliation will he enough. I'll update as I go.

Welcome to Day 50 2/5/21 My god is she tall, thank god she stopped stretching. As you can see in the pics she has a great many tops and bud points, fingers crossed she fattens up soon. I decided to add deep clean to the solution to help with the potential of salt build up. they are drinking a lot 18L every 6 days and that's a good thing, I also upped the big bud from 1 ml a L to 2 ml a L If there are any questions feel free to ask and as always happy growing and keep your stick on the ice 🙏

She is going to be huuuuuge! All branches and stems have risen up and are strengthening daily in order to produce big resin filled flowers! I love her progress as she climbed to the height of the purple punch and soon to stand taller! Can’t wait to have the final weeks approach! Thanks for stopping by buds and chuds 🤩😎💪🏽

Blütewoche 4 beginnt Die Harzproduktion ist der Wahnsinn

Wow I'm in bud heaven. Both plants are doing good especially#2. # 2 is absolutely stunning, covered in frost and getting very chunky buds. Her growth has been spot on since germination. She has displayed good hybrid vigor and in honest opinion Barney's farm has nailed it on this genetic masterpiece. #1 is now stacking very nice flowers as well being that all her troubles are gone thanks to a couple of good flushes and some fresh organic amendment. Her flower structure is very different from her sister, more polyploid than than the traditional structure. I have to admit that she is becoming a very beautiful specimen of a cannabis plant. CHEERS!!!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

11-1-2025 she is looking great 1 day pass schedule harvest date. Im push her until a sign to show and tell cut her down beautiful out. It was pretty difficult getting the video with the trichome. Holding the jewelry loop on the back of the phone, being steady ✨️ 👌 takes work

Easy grow. Special qeen best od Royal Queen seeds. Next year again ..... special qeen 2 1 week to Finish

Definitely would’ve been better had I not moved but nonetheless it was a great run overall, super frosty and sticky with not very dense buds which made for a difficult trim but I didn’t mind.

en lineas generales bien el cultivo. la genetica es buena aunque no esta muy estabilizada. no es resistente a los hongos, la cola mas grande que estaba vencida sobre otras plantas tiene botritis ne la parte baja de cada cogollo con lo que se habran perdido facil 30g en seco.