ESPAÑOL: Hola chicos, espero se encuentren muy bien! Esta semana las chicas han mejorado un poco, se detuvo el amarillamiento, descubri que Cal-Max de Grotek interfiere en la composicion de la linea hesi por lo que las chicas sufren un bloqueo de nutrientes, dejé de utilizarlo y las chicas han continuado con su desarrollo relativamente normal. ya estan en su 5ta semana de floracion, Tutankhamon ha producido una cantidad gigantesca de resina, Lennon esta engordando a toda velocidad y aun le quedan 6 semanas aprox. Anubis a pesar de su color intenso amarillo palido esta engordando bastante bien y su produccion de resina es inimaginable y ya solo que quedan 2 semanas y media aprox. espero que a partir de ahora ya no surjan mas problemas y que las chicas terminen su floracion de la mejor manera. esto es todo por esta semana chicos, espero que se encuentren muy bien ! INGLES: Hello guys, I hope you are very well! This week the girls have improved a little, the yellowing stopped, I discovered that Cal-Max from Grotek interferes with the composition of the hesi line so the girls suffer a nutrient lock, I stopped using it and the girls have continued with their relatively normal development. They are already in their 5th week of flowering, Tutankhamun has produced a gigantic amount of resin, Lennon is gaining weight at full speed and he still has about 6 weeks left. Anubis, despite its intense pale yellow color, is gaining weight quite well and its resin production is unimaginable and there are only about 2 and a half weeks left. I hope that from now on no more problems arise and that the girls finish their flowering in the best way. This is it for this week guys, I hope you are doing well!

Hi everyone :-) This week a lot of nice things happened in the flower tent :-) Everyone looks super nice, and is growing stronger and more beautiful week by week 😍👌. The blue cheese smells like a dream ;-) As usual from this variety 👍. The kosher tangie is also very, very tasty 👏🏻. Both got Pk 13/14 this week for the last time :-) Everyone else is developing very well 👍, That will be the last diary with several strains together :-) In future everyone will come individually 👌. I wish you a lot of fun with the videos, have a nice weekend, stay healthy 🙏🏻 and let it grow 😎👌

Flowering process. Good weather

Привет друзья. Моей растихе сегодня 129 дней Сегодня температура упала до +8° Замёрзла моя девочка(( Буду делать сверху водяное отопление) а то так дело не пойдёт зимой) 20.10 перевёл свет в режим 12/12 Начал применять LST технику на 19 дне, а 18 августа добавил ДЕФОЛИЗАЦИЮ С 20.08 ДЕФОЛИЗАЦИЮ делаю каждые 3,4 дня С 20.08 LST технику делаю каждые 4.6 дней На сегодняшний день влажность 54% 5.09.2023 заметил высокий Ph 7.9 С 48 дня Ph не ниже 5.8 На сегодняшний день Ph 6.0 Начал кормить с 60 дня Canna Terra Vega PPM 870 Всем мира и добра! Не забудь поставить лайк❤️, если понравилась как прошла неделя И читайте наш TELEGRAM: https://t.me/smail_seeds #Smail_Seeds 😀

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Vaso de 750ml, 12 dias de vida e 9º dia na iluminação! Está se desenvolvendo rápido. Raizes fortes e bem ramificadas . Adicionei 0,2 ml de top veg pra ajudar no vegetativo Temperatura elevada, durante o acender das luzes 30C°

Welcome to week 4 of my Super Silver Haze diary (day22 from germination) Genetics by: Zamnesia seeds Looks great, nice and tall already. Going to flip to 12/12 schedule today, otherwise it will get bigger than 1meter. Last watering was last week, waiting for the soil to dry out a bit before next watering.

15-05-21 Se cambia la solución y se realiza un lavado de raíces. Para bajar la conductividad de las raíces de 1.43 a 0.2 ms para que la solución nueva sea más efectiva. La solución tiene 2.23 ms y 6 PH. Se añade una pastilla de CO2. 16-05-21 PH 6.2 Conductividad 1.85 ms 17-05-21 PH 6.1 Conductividad 1.84 ms 18-05-21 Se corrige el PH 5.2 a PH 5.9 Conductividad 1.97 ms. Se rellena con agua del grifo ya que el nivel es bajo. Conductividad 0.92 ms 19-05-21 Se corrige el PH 5.6 a PH 6.1 Conductividad 0.97 ms. 20-05-21 Se corrige el PH 5.3 a PH 6.30 Conductividad 1.21 ms. Se rellena con agua del grifo ya que el nivel es bajo. Conductividad 0.74 ms 21-05-21 PH 6.10 Conductividad 0.78 ms

I gave her a good trim. Fed her some bat guano juice. Now she is drying off for 2 days to use up that power…you can smell her 4 feet incoming.

Dinafem Cheese 1st week of flowering 10th june 2020 Well after 5 days since switching to a 12/12 lighting regime this lovely girl is starting to bloom. 👌 😃 I gave her a feed of some Blackstrap molasses @ 1 teaspoon / Gallon (mixed with water) also added in Ozi Magic Monstabud @5mls/L She hasn't stretched too much which is a relief to me and I'm now looking forward to watching her buds develop over the coming weeks 😎 the Marigold I planted as a companion has flowered now also and is a brilliant yellow colour Thanks for stoppinng by 👍

It's time to taste it. Sweet scent with a distinctive identity. You guys have to try to touch her once, I hope so. :)💚🥦

This Week they grow up good! I think i ll put This for flowering because I dont have space enough!! 👊🏻👊🏻☘️☘️

tercera semana de floración en la cual las flores ya empiezan a resinar y formando su estructura de la flor / third week of flowering in which the flowers already begin to resinate and forming their flower structure

Day 29. No feed still. I spray them with Bio Heaven and CalMag light mix, sometimes adding a bit of Silica acid too. All not higher than 1ml/L. Think this week I will try to push best ones, smallest coming up too, but very slow, droped them to the shade and don't put any hopes into them. Day 33. Did little repositioning, space looks better now I think, plus big plants should be happier. Spray spray spray ;)))) Happy Growing !!!

Day 70- Switched to plain ph’d water mid-week, they have now had 2 waterings with plain water. Probably going to be 2 more in the upcoming week. Did a big defoliation mostly to help ease the final trim when it comes, but to also allow for a little more trichome development as she uses up the remaining nutrients. It’s been a much cooler week so I have been gradually increasing the light intensity to get a final push for some weight. Running at about 95% intensity at the moment with the tent hovering around 79-81 degrees freedom units. Checking trichomes daily with my 30x jewellers loupe, mostly cloudy now, some amber popping up, but mostly on the leaves. I’m watching my other strain as well, hoping the timing will be right for both to come down. I’m not in a rush, really want to maximize these buds, they are already very hard, and they will be decent producers.

Day 57: Watered each plant with 1L with nuts 1588 ppm, 3380 us/cm, 3.3 EC (purple punch, strawberry banana, wedding Cheesecake) 1690 ppm, 3595 us/cm, 3.5 EC (gorilla cookies) 2 different feedings for the 10 plants Day 60: Watered each plant with 1L with nuts 1563 ppm, 3325 us/cm, 3.3 EC Day 62: Watered each plant with 1L with nuts 1726 ppm, 3712 us/cm, 3.7 EC (purple punch, strawberry banana, wedding Cheesecake)(I gave them more than usual, by mistake) 1528 ppm, 3525 us/cm, 3.5 EC (gorilla cookies) 2 different feedings for the 10 plants Day 63: Watered each plant with 1L with nuts 1563 ppm, 3325us/cm, 3.3 EC (purple punch, strawberry banana, wedding Cheesecake) 1523 ppm, 3301 us/cm, 3.3 EC (gorilla cookies) 2 different feedings for the 10 plants Day 65: Watered each plant with 1L with nuts 1518 ppm, 3210 us/cm, 3.2 EC (purple punch, strawberry banana, wedding Cheesecake) 1359 ppm, 2891 us/cm, 2.9 EC (gorilla cookies) 2 different feedings for the 10 plants Next feeding I will start to flush some plants, 1st week with flawless finish, 2nd week clean water, 10x the pot, 150L each Day 67: Watered each plant with 1L with nuts 1379 ppm, 2908 us/cm, 2.9 EC (1 purple punch, wedding Cheesecake) 1250 ppm, 2687 us/cm, 2.7 EC (gorilla cookies) 285 ppm, 606 us/cm 0.6 EC (3 strawberry banana and 2 purple punch) (2L each) 3 different feedings for the 10 plants Started to flush all the strawberry banana and 2 purple punch with flawless finisher. (1st week flawless finisher, 2L each, 2nd week clear water, 150L)

Week 8 in the books, cruising to the finish line two more hard watering each and 48 hrs of darkness starting Friday. Genetics: Natty Roots Seed Co Instagram: @green_house_lab

So... I may have got baked and had one of those.... "I know what will be a good idea.!" I give you Frankenstoned early Lst lol I know I am a novice and have no right to be messing with things that already exist, and the weighted lst has probably been done many times already but I was having fun, I had a silly stoned idea, and just for shits n giggles, I made it happen, and for me this growing thing has to be fun right? Humour aside, it actually seems to work lol. I find I am a little heavy handed and a bit clumsy trying to tie things down. I recently accidentally snapped one of my criticals branches off so my little system allows me to just lift the wires up and tuck it under at strategic points.. It is quite early to be training I guess but the wire gently pushes down on the stem.. It looks heavy handed but it is actually quite gentle... So let's see if it works or not.. I am only trying it on this 1 subject.. I am expecting for the Mazar to pull up on the weights as it fattens up but I have allowed space on the bolts to add more weight.. Other than this revelation (or massive cock up) lol. The Mazar has been struggling in the heat a bit but surviving.. Let me know your thoughts, happy growing lovely people

Muchas gracias por dejarme participar BsF y grow diariaries y compartir este cultivo gorila glue 4. Fue un hecho en scroog en un metro veinte cuadrado usando dos masetas de 23 ltrs usanso microorganismos y productos orgánicos súper contento con esta sepa, fueron criadas con dos focos galoponeros en un armario con poco a lo q tengamos podemos hacer mucho lo importante es aprender un poco todos los días y disfrutar de nuestras cosechas un fuerte abrazo para todos @gorilaweed420

Gradually, Gorilla Cookies begins to extract the nutrients from the leaves. it is impressive how much resin it produces. Hopefully she will do well in the last few weeks.