Filling out the trellis net. Looks like another 2-3 weeks until I flip the ladies. Using SST’s, LABS and natural inputs for this grow. I have made some mistakes in the past using Organics, but have figured out HOW to use them for an entire grow. The vegetative state, I have never had a problem with. The transition into the first 3 weeks is when I have had the most issues. After a little research and testing, I finally have a basic blue print of how to feed plants in flower for 10-12 weeks now or shorter. Mark Bowell from Perfect Gardens on YouTube and Mr Canucks Grow from Canada are my largest information inputs. Final Thank you to Mr McGooz for keeping me in the know. 10-11: yes, some of the stalks are turning purple. This is not due to phosphorus, magnesium, or any deficiency. It is due to the light being too intense for the plants. I am at the max distance and running the light at 30% ( it’s a 600 watt 🤣🤣). Soooo yeah, that purple is going to stay for a bit. Purple with other leaf discoloration or senescence IS a problem, purple stems with NO leaf issues isn’t serious issue. Cant it be fixed? We are going to find out….Photosynthesis Plus will be on hand in the next 5-6 days. This permits the plant to USE more of the photons ( yes, I have carbon in my media). This should minimize the purple and allow me to UP THE WATTS 🗣️🗣️ 10-14: spoke to my “guide” and we made the decision to go ahead and flower these ladies. We saw how much stretch there is to be had and realized we are currently filling the trellis by 75%. We are estimating the entire 4x4 area to be completely filled. I defoliated and pruned the smaller sites off the lower areas of the plant. Not quite lollipopping, just selectivity choosing sites to keep and bones that will get in the way. For transition I am using a ratio of 1:1:1 on NPK’s to ease the flip. The ppm’s will be between 750-800. Once we reach early flower, we switch the NPK’s to 1:3:2 ( flower girl by Dr Earth OR Fish bone meal and langbenite). I switch each feeding between the two for 5-6 weeks. At week 7 we switch to 0:3:3 ( seabird guano and Langbeinite) at 500 ppm’s. Week 8 the same feed. Week 9 kelpseaweed/water Only ( these are 10 week cultivars). Thank you for taking the time to read. 10-16 😍😍😍 Plants are starting to show deficiencies!!! Yes, normally one freaks out 😂😂😂 The slight deficiency is a sign that I can go ahead and start: The Flowering Cycle. When using Organics, you have to use your eyes because nothing is specific. Meaning: you have to see when the amendments are running out to A: Top Dress B. Make compost teas. We are going the compost tea route for flower. I made a video showing exactly what I use. The ratio is 5-5-5 ( yes earlier we were going to use 2-2-2, but we found out there is no calcium or magnesium in the all purpose mix by Dr Earth…another reason to learn to read your plants).

All is great with my ladies this week the buds are fattening up nicely I got alot of bud sites and they are all developing beautifully the 2 plants that were approximately a week behind are also now fully flowering thankfully The extractors are working hard keeping the humidity low temps are around 28.5 lights on and humidity is 42 they are drinking more and they are getting the full recommended monkey nutrients and the Zues pro 600 is on 100% finally. Let's hope the ladies keep improving Stay blessed 💚

Day 15 🌱 everything is going well but I have accidentally decapitated N1, in reality I had topping already N3 but with N1 I broke without doing it on purpose. hope for the best. Day 16🌱 wow today N1 I see it well it does not seem to have accused the topping. everything seems to be going well for N3 too, he seems to be growing very strong. N2 is in excellent condition. Day 18 🌱 i am struggling a bit with "lst" but i guess because i started early. otherwise they seem to be growing well Day 20 🌱 I let them rest for two days. some training again today, they look fit and grow great. Day 21🌱 N1 and N3 grow great and look great. N2 grows well but has yellowed leaf tips as if I had given too much fertilizer, I think due to the pre-fertilized soil. Now I continue with water and I will check the drain ph Thanks for viewing. if you like my diary leave a like and a comment. come back to see me 🎂 good cultivation and good day💚🌱

Guava Sundae – Week 10 (Fast Buds Outdoor 2026) The weather was okay this week. Guava Sundae is almost ready and is therefore being fed less now. She is fully in flower. The aroma is already extremely strong and very pleasant. Some of her leaves have developed a slight purple tint. I checked the trichomes again and they are much more milky now. Quite a few are already turning amber. No more nutrients from now on as harvest is approaching soon.

Continuing to water almost everyday as the pot size is too small and the buds are packing on the weight and frost. I am giving them almost 4 liters of water everyday. I am going to skip today (monday) just to make sure im not promoting any root rot, although I know I will be sacrificing some microbes. I will water them on my Live first thing Tuesday morning. Im noticing a little bit of bronzing on the leaves of one, its something I get almost every grow in flower. Im thinking it is beacuse I need to change my RO filters, as I live in the country and use well water and I think It is a buildup of iron. I have crazy high iron even when I use an RO. I believe my TDS was still 400 with filtration lol. If I change the filters I should be able to get it back down to 100 to 150 ppm. We are on auto pilot right now with this garden, just have to water and nurture and I know I am going to get an amazing result in about 3 weeks or so!

Blütewoche 6 Die volle Blüte Noch gibt das Blümchen vollgas und die Blpten schwellen weiter. Da sich aber die Trichome schon milchig färben wird die Düngerdosis bereits reduziert. In ein bis 2 Wochen kann geerntet werden. * Zustand der Pflanze: Gesunde Pflanze mit immer dicker werden Buds, * Wichtige Ereignisse: Reduzierung des Dünger * Düngung & Messwerte: * Gießwasser: pH ca. 6,3. Leitwert 0.8 mS. * Drain: Wir stellen sicher, dass der pH-Wert nicht unter 5,5 und nicht über 6,8 liegt und der Leitwert die +/- 500 S Abweichung einhält. * Anpassungen: Aufgrund des Eigengewichtes der Buds mussten an Tag 88 (Blütetag43) Stützen eingebaut werden.

I feed light the first week then I bring the ppm up to about 1050 to 1200 slowly cause we all knw that these nutrients can burn and will

NANA GLUE AND ICED WILDBERRY PART 2 RETURN OF THE CLONES NEXT THIS WAS FROM SEED

Week 7 Although the Gelato is the smallest of the bunch it's definitely the fastest flowering. Also I'm trying out a little experiment if the results are positive I might share more about it in the future..

Feb 22 Really starting to thicken up! The soil underneath is still plenty moist even though the fan is blowing on it 24 hours a day. The roots will grow more quickly as the water dries up and the roots continue their search for more.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Verpasste Woche…

day 28 this week the has started strech for what i can see.. from now we can consider to be on bloom phase i only gave them water with calmag and no much more... maybe this week i feed them with top auto but they look very healthy ATM but like the wáter intake is going Up.. solo. Will need some feeding.

G Z 1 In first week of flower G Z 2 In 3rd week G Z 3 first week G Z 4 in first week G Z 5 in second week of flowering G Z 6 also in first week should i count their weeks like this or consider all at first week of flowering? I am not good at flowering stage before! But i have hope for this gro

We are just waiting on the trichomes. I will probably cut her down next week. In a few days time. The calyxes have not fattened any more. Here is what I did this week. 17.8.25: I watered with 2.5L of dechlorinated water PH'd to 6.5 with; 💜 1ml Trace PH: 6.5 PPM: 355 21.8.25: I watered with 1.5L of dechlorinated water PH'd to 6.5 with; 💜 1ml Ecothrive Flourish 💜 1 TSP Biosys PH: 6.5 PPM: 354 Thank you for stopping in this week and hanging out 😁💚✌️🍃🙏

Blueberry Lime Kush AD

This strain has honestly been amazing growing outdoors. Very resilient, amazing trichomes production and bud structure, have had to perform daily inspections to prevent bud rotting and mold spreading however with such a rainy season I will look at this year as an overall success. One more week to go in order to maximize yield and trichomes production

FB getting sticky and stacking on weight , she’s a beautiful honey colour , just need to do little defol but it’s getting hard to move around in there … good prob

Got a new light this week, it's basically a HLG 260 R-Spec except locally manufactured using the same Samsung LEDs. Big Mumma got her 60x60cm tent today and will go in there under one Viparspectra 300W blurple (130w from the wall). I trimmed her, super cropped her main stem and added a scrog net in preparation of flowering her in the next couple of days. Because my other plants were getting up past 75cm after 7 days of flower (100cm is my absolute max) , I decided to supercrop the canopy back down to about 60-65cm - it was pretty scary and I definitely broke the outer stem on some of them. Also gave them a good trim. Fortunately most bounced back strong the next day which are the most up-to-date photos you see (17/4/20).