All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

This plant has been my strongest/largest from the gate, so no surprise she was my biggest yielder! Incredibly strong/resilient from the start, however everything seemed to just go right for this plant. Lots of defoliation and attention helped cultivate dense medium-large nugs that certainly pack a punch! Very flavorful, and amazing aroma. I will definitely be growing this strain again!

This is my first coloured cannabis strain and this red critical Autoflower from BSF seeds is an amazing strian. The smell of the buds is so fruity. Cant wait to harvest this red plant.

I fed her 100ml of phd water due to wilting leaves I will wait for the pot to dry up again before I feed her again! The next feed will probably be her last before I repot her. She still is receiving natural sunlight!

Day 85 / Flip 46 / 24.3 Flushed them again with EC 0,0 water. EC Drain is now on 0,0. The next step is to let them dry out. Nutrients will get soaked out of the leafs now, which will make them turn yellow. Letting the plant die now with full light power. I'm planning to harvest in 1 week. Grow is running nice,. Plants look super frosty and will even get more in the drying stage now.. yayyy.....:) Day 91 / Day 52 / 30.03 Additional stress with a 48 hours no light stage. harvest will be at 1. APRIL HARVEST!!!! 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL HARVEST!!!! 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL HARVEST!!!! 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL HARVEST!!!! 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL 1. APRIL

La primera semana de floración les sentó bien la echaron su sexo, hembras, ahora ya queda ir viendo cómo se forman las flores. La temperatura no pasa a los 30 nunca.

🍃🌱🐛🌞🤭

Day 30 (Week 5 day 2) watered the girl with bactrex 1g, batboost and biogrow she has recovered from day 26 that was when i transplanted her in the final pot that is 1,8L Day 34 (Week 5 day 6) today i have topped the girl again because i felt she needs it for more colas that i will lst. And i have watered her with batboost and fishmix.

Not much to report apart from some decent growth, she’s starting the stretch a little. New ph meter and EC meter here now that really help with nutes and watering accurately :) Thanks for reading and happy growing!

Timelapse Days 21-34: Memory card filled up and I lost days 35 and part of 36. Day 36: No photograph. No longer centered under the light. I need more lighting. Day 37: plant taking to the training well. Almost time for another tent peg. Day 38: Still growing. Day 39: Worried this is mold. Added a gallon of water with nutrients increasing the nutrients to mid-vegetation. Overall I think the plant looks pretty healthy. Day 40: Turned the carbon filter on to promote air exchange. Day 41: Continued training. Day 41: Rushing the exhaust fan has reduced the humidity. Going to have to keep an eye on that.

Better than what i think.. i love banana chikita very good strains 👌💨💨

Let´s start a new week 😎👌 Day36 very sunny and warm... "Hilde 2.0" goes well 😎👍🙏 Day37 starts with massive cloudy weather but later it turns into a sunny afternoon 😏🙏 Day38 was cloudy and rainy and not so much sun around here 😳😵 Day39 damn cloudy and rain....wth is this summer doing 😵😧 Day40 wth is wrong with this summer 😡 cloudy and rain and no sun 😵😨 Day41 little bit summer came back 🙏 cloudy but with some nice sunny times 😎👍

Blue Cheese F1 automatic Week 5 2/2-2-8 The Cheese gals are in full flower and are stretching like crazy. At the beginning of the week, they were 7 inches tall, Wed 10 and today 2/7 they are 14 & 15 inches tall. They have doubled in height. I’ve moved the pins out to the edge of the pot. For future training, I must add some wires thru the pot holes to keep the branches lower as the outer rim is taller than the center. I reduced nutrients in the feed solution since Runtz and Pink Rozay are showing some purples/blues in the leaves. These gals are vigorous and don’t seem to mind the full strength feedings. Hopefully they won’t start showing deficiencies. I added Green Sensation this week then read the instructions said add in 4th week flower. Blue Cheese is supposed to be a 10 week flower from seed to harvest. If I wait till the 4th week, it will only be used for 2 maybe 3 weeks. Would that actually benefit the plant. So I think I will add some each time but a 25% strength. Calls for 4ml/g I will use 1ml. They are autos and I should be lowering the nutes anyway. Pinned more branches. I add 2 gallons of water to the T700 humidifier daily to keep the VPD in line. 2/5 4 gal fed 2 liter each nutrients added at ½ of recommended amount. Making 4 gallons since I have 2 pots of carrots and Dill in the back. Bloom Juice 60ml Plant Juice 60 ml Royal Rush 15 ml power bud 15ml green sensation 7.5ml Recharge 2.5ml/gal 7.5 ml Ppm 333 Ph 7.02 Temp 66 2/7 3 ltr each Bloom Juice 60ml Plant Juice 60 ml Royal Rush 15 ml Power bud 15ml Green sensation not added Ppm 298 Ph 6.5 Temp 65 Your likes and comments are appreciated. Thanks for stopping by. Growers love 💚🌿 💫Natrona💫

This week I defoliated slightly for better air circulation and improved light penetration, mainly removing leaves blocking budsites that had purple petioles. They seemed to respond good. Also foliar sprayed with light potash solution, upped pk with guano and provided micros with seaweed extract. Feed EOD and water with silica in between feeds. RH now around 50-50% most of time. Improving air circulation is now top priority on to-do list

Crecen sanas y bien. He añadido un led de 100w Spider Farmer sf1000 💡 🕷️ 🧡 Veremos los resultados

Transplant successful and my babies are thriving, even braved the cold this week, however we are not out the woods yet, I think not having a fan is hindering my plants, conditions are favourable for my babies, let me not stress, I will make a plan, sacrifices must be done… for the greater good. The positive is my grow medium mix looks like it was well balanced, I need to buy 20 bags this time, and add it to my veggie patches, and I will not be defoliating anyone this month, fan leaves’ green colour is lovely and uniform, she is happy with her diet, and you will see the uniform green colour on all these ladies, except for Monday’s Blue she had a lot of nutrients in the beginning, but all is bright and right.

Hello people week # 8 at the start. I fertilize 3 more times this week with a liter of water (6.5ph). Hope this week she finally develops the colas a bit. I had a little mildew problem last week, but defoliation resolved the issue. It fits perfectly into the season from the look hope dan to get good gifts. Therefore I will start my flush in week 9. I'm curious what will happen in the next few weeks. Thank you for your time. A fox does what a fuschs has to do. 🦊🦊💚💛❤️

Harvest was quick and painless!) At this point, the plant hangs upside down and dries slowly.