Beginning of week.. She is doing amazing! Runoff is right where it needs to be.. leaves are looking nice and green with not so much leaf burning on the tips anymore.. new sets of leaves daily! Gonna need to lollipop a bit more. She should produce a decent harvest! So excited to see what she does in flower! So far, I've noticed that she is making some purple striped stiples at the very crown of each (future) cola! So so excited! Need to raise light up a bit.. I think she's getting over 1000 micromols at the center sine she has stretched a bit... Next day.. Whoops.. not sure how the hell she managed to stretch up to 1400+ micromols so quick.. had to raise the light quite a bit and also dim her down just a tad.. now the center is getting exactly 950 micromols in the center and around 650 - 700 micromols around the edges of the tent (footprint). Perfect! I noticed some purpling on a couple of the higher leaves.. these were the leaves getting over 1400 micromols and temps have been another lower than anticipated. So if she starts turning purple this early on, then WOW!.. She should make some pretty colorful colas! Mid week.. She really needs a haircut. She has grown a few nodes on each branch just in the last 7 days or even less. So beautiful! Humidity is being a bitch right now. Up to 70% and my dehumidifier can't even get it to drop a little.. ugh. Cold front blowing through as I type this, so hopefully it'll blow this moisture out by the end of the day. Pistols should be making a crown pretty soon! Still stretching like crazy. She still has plenty of head room for it.. but damn, I really need to get some of these leaves and lower branches out of the way. That should help bring the RH down quite a bit too.. I know she isn't THAT big, but it's still gonna take me awhile to trim her up.. kinda wondering if I could wait till next week and get it all done at once.. cuz I feel like she may need one more with the speed she's been stretching at. Next day... Went ahead and removed ALOT of foliage.. humidity should go down quite a bit.. these leaves give off a very strong skunky scent.. can't wait to see the terp action of this strain! "OoOo.. I 'member"

Showing signs of flower. Checking for stress seeds or hermie plants every time I water. So far so good though. Feeding schedule seems to be working great. I'm gonna really hit em hard with the florabloom next week.

Happy Mother’s Day. 5/13 Getting weird like bright neon orange pistils. I personally think it’s way too early to have pistils changing colors already. 5/16 fairly certain it was wind burn. My humidity was jacking up and my fan was trying to compensate. Changed settings and we should be golden now. Plant is frosting up hella already. I think there’s more frost than there are pistils 5/18 day 20 of flowering. Last day of week 2

OG 4Q24 Seedling Bolt III Orangegasm (Fem) [ IRIE Genetics ] 12/12 @ Bolt (Day 21) Germination: 20 November 2024 #3A Earliest Harvest Date: 9 February 2025 #3B Latest Harvest Date: 19 February 2025 _________________________________________ Start of Week: [Wed Dec 25, 2024 CR2 4Q24 1:B:1:1] End of Week: [Tue Dec 31 , 2024 CR2 4Q24 7:B:1:7] OrangeGasm Fertigation: Max EC: [ 2.4, mS] _______________________________________ Week’s Objectives: - IFF We’re still HAND WATERING, ADD First Resin Bloom to Full @ 4ml/gal. - We can’t replace RLA’s Carbon Component with/out ~300 ml/Gal Ful-Power (Fulvic Acids) We WON’T be doing that. Rather, we’ll see a diminution of Carbon provided, look for 3-5 days to stabilize after switch to auto-irrigation. EC Starting Water: ec: 0.0 mS/cm, pH: ~7  __ Wed Dec 25, 2024 # Christmas Day OG 4Q24 15:B:2:1 Watered late tonight (~2200). - NO RUNOFF - Barely the beginning of Burnt Tips - Grew 2” overnight - raised light 2”, Canopy Distance is 19.5 @ 77% (770 ppfd) After Watering - With REDUCED Light, Plants are FRISKY Leaves raised (but no fingers in light sockets) - # We’ve got Pistils (Noted Yesterday) - Ceasing Foliar . . . IT’s been good. ;-} Runoff: NO RUNOFF Fertigation: - [x] Primer A&B: [ 10.2, ml] - [x] CalMag Fuel: [ 6.8, ml] - [x] Silica Skin: [ 6.8, ml] - [x] Lush Green: [ 5.1, ml] - [x] Root Anchor: [ 5.1, ml] - [x] Peak Bloom: [ 5.1, ml] - [x] Photosynthesis Plus: [ 6, ml] - [x] Quillaja 60 Powder: [~scant~] - [x] SLF-100: [ 5, ml]  Standardizing Lighting __ Thu Dec 26, 2024 # BOXING DAY OG 4Q24 16:B:2:2 Scotoperiod: [ 12, h] EC: [ 2.4, mS/cm] Temp: [ 79, °F] RH: [ 68, %] VPD: [ 0.85, kPa] NOTE: If we’ve no runoff today, Water AGAIN at Midnight, Install Auto-Fertigation Tomorrow Raised Light 6 Clicks - Grew 2” Overnight - Light Distance ~20” @1900 - Fertigation Fertigation: - [x] Primer A&B: [ 10.2, ml] - [x] CalMag Fuel: [ 6.8, ml] - [x] Silica Skin: [ 6.8, ml] - [x] Lush Green: [ 5.1, ml] - [x] Root Anchor: [ 5.1, ml] - [x] Peak Bloom: [ 5.1, ml] - [x] Photosynthesis Plus: [ 6, ml] - [x] Quillaja 60 Powder: [~scant~] - [x] SLF-100: [ 5, ml] IPM: - [x] Zymes Bomb - Top to Bottom, including Airpot Sides.
Foliar DRENCH w/ Zymes at 1:31 water dilution. Drenched top and bottom of leaves, stems, JOINTS, Coco Top, and spraying down ALL Airpot Side Holes, and BOTTOM OF AIRPOT. Letting the girls run up to 82°F, RH @ 69%, VPD ~9. Plants are HAPPY w/ EC: 2.4, w/ Moderate High Lighting (~770) If they’re happy before lights out, and I know it - Let’s raise light to 80% (800 µMol/m2/s), adjust airflow for overnight Temp (Humidity Down). And call it a night. After Raising light to 83%, REDUCED again to 77% @0600 __ Fri Dec 27, 2024 OG 4Q24 17:B:2:3 Runoff: [ 500, ml], EC: [ 1.7, mS/cm] Raised Light 6 Clicks (Grew 1.5” Last Night). Light Distance back to ~20” 1.5 Gal fertigation - let’s do 1900 & 1200  Fertigation: 1.5 Gal # 2000 & 0100, .75 gal Each, EC: 2.5 - [x] Primer A&B: [ 14.8, ml] - [x] CalMag Fuel: [ 9.9, ml] - [x] Silica Skin: [ 9.9, ml] - [x] Lush Green: [ 7.4, ml] - [x] Root Anchor: [ 7.4, ml] - [x] Peak Bloom: [ 7.4, ml] - [x] Resin Bloom: [ 4.9, ml] - [x] Photosynthesis Plus: [ 9, ml] - [x] Quillaja 60 Powder: [ 0.125, tsp] - [x] SLF-100: [ 7.4, ml] After 2:30 Feeding - Raised light to 83% __ Sat Dec 28, 2024 OG 4Q24 18:B:2:4 Temp: [ 82, °F] RH: [ 68, %] VPD: [ 0.96, kPa] Light Distance: [ 19.5, “] Fertigation: RLA Heavy, Flower Week 3; EC: 2.4 Notes: - Evaluate 83% Dimmer at Lights On - Back off to 77% if any indication of Light Stress - Let’s ramp to EC: 2.7 mS As max. Beyond here, we probably don’t have sufficient CO2. Light Intensity (IDEAL): 83% # ~830 µMol/m2/s EC: 2.4 422 ml/plant, runoff, EC 1.5. Fertigation: 1.5 Gal # 2000 & 0100, .75 gal Each, EC: 2.5 - [x] Primer A&B: [ 14.8, ml] - [x] CalMag Fuel: [ 9.9, ml] - [x] Silica Skin: [ 9.9, ml] - [x] Lush Green: [ 7.4, ml] - [x] Root Anchor: [ 7.4, ml] - [x] Peak Bloom: [ 7.4, ml] - [x] Resin Bloom: [ 4.9, ml] - [x] Photosynthesis Plus: [ 9, ml] - [x] Quillaja 60 Powder: [ 0.125, tsp] - [x] SLF-100: [ 7.4, ml] 420 ml/plant op Crinkly LOWERS - EC Too High Too Fast? __ Sun Dec 29, 2024 OG 4Q24 19:B:2:5 EC: 2.3-2.4 The Max Hydro comes in at 2.36 EC. RLA Hydro Chart set 3.4. How Can Be? DROPPING SILICA SKIN from RLA Hydro (Eliminate Brown Patina, and Small Orifice Build-up) RLA Hydro  Nutrient Element Ratios ppm mS/cm NER N 262 0.5 21.8% P205 143 0.3 11.9% K20 476 1.0 39.7% Ca 214 0.4 17.9% Mg 48 0.1 4.0% S 57 0.1 4.8% Si 0 0.0 0.0% C 333 TOTAL 1533 2.4 100.0%  
RLA - Full - [x] Complete Irrigation Install Defoliation I We want to eliminate All Vegetation from TOPPED NODE (3) Down. - [ ] Count NODES FROM SPLIT - [x] Measure Plant Heights: 11 - 17” - [x] Remove Each Pot and Trim OUTSIDE OF TENT (Get it RIGHT!) - [x] Can we adjust the pot positioning to get ONE UNDER CANOPY Fan? - [x] Eliminate FANS and Growth tips NODES 1 - 3 (3 is TOP SPLIT) - [x] Eliminate FAN LEAVES from First Node of MAIN STEMS - [x] INSTALL DRIP RINGS (No Manifold, Yet) - [x] Apply LABEL to Each Plant - [ ] Consider Eliminating FAN LEAVES from Second Node of Mains _______ Fertigation: 1.5 Gal # 2000 & 0100, .75 gal Each, EC: 2.5 - [x] Primer A&B: [ 54.1, ml] # EC: 2.4 MAX - [x] Photosynthesis Plus: [ 24, ml] - [x] Quillaja 60 Powder: [ 0.25, tsp] - [x] SLF-100: [ 15, ml] Time Set Initiate Complete Event 1 18:45 18:45:25 Event 2 20:05:00 20:05:25 Event 3 21:25:00 21:25:25 Event 4 22:45:00 22:45:25 Event 5 00:05:00 00:05:25 Event 6 01:25:00 01:25:25 Event 7 02:45:00 02:45:25 Event 8 04:05:00 04:05:25 Event 9 05:25:00 05:25:25 CHANGED TO 25 SEC EVENT (from 20) Tuesday - / / / __ Mon Dec 30, 2024 OG 4Q24 20:B:2:6 - [x] Harvest Dehu Water Continuing to RAISE LIGHT ~ 2”/day (6 Clicks of the support ratchets). Runoff: - [ 422, ml, plant!!!!] # Reduced Event duration to 20 Seconds (from 30). - [ 1.4, mS/cm] Lowering light to 70%, see if lower fans relax. They’re electric. :-). They relaxed. Inner fans surrounding apical stems are laying @ 30°, rather than Reaching for 60°. I have perhaps been overnighting. HUMIDITY IS CRANKING. It’s impossible to get VPD above ~8.3! Trying to keep humidity below 68 drives Temp below 78. I forgot about the part. ;-( Dehumidifier is ON, Doors are Closed, Room Vent is CLOSED. Dehumidifier is delivering 50%RH to the room. Orange Gasm is quickly filling the air with moisture. At MIDNIGHT, Raised light to 77% ( ~770 µMol/m2/s] pH Adjusted 2 Gallons of Filtered Tap to pH: 7.2, EC: 0.3 (Used for Dilution when refreshing reservoir with DEHU and Fresh Supplements. __ Tue Dec 31, 2024 OG 4Q24 21:B:2:7 @0630 Took a peek in the tent. SCANT RUNOFF @ 20 Sec Feed Events. JUST Touched TIPS (Burning). Bumping Fertigation Events to 25 Seconds, after Feeding 9 * 5sec (45 Sec) To bring DAYS delivery to 25 Seconds. Check Reservoir: - We’ve 4 - 5 Gallons. @LIGHTS ON - Reset Light Height - Reduce light to 73% (See if we can avoid more tip burning) Harvest Dehu: - [x] Amount: [ 5.7, l] - [x] Primer A&B: [ 27, ml] # For EC: 2.4 mS/cm, Nailed it, First Try. ;-} Orange Gasm likes to SPREAD when Topped. - Which makes her a funny candidate for SOG. ;-} 

+ Day 75: Harvested !!! Now Drying !!! +

During the middle of this week I decided to stop the outward training of the branches & the allow vertical growth for beginning bud site formation . I don’t want to miss the beginning flower stretch kinda need it 😂. Decided to break the apical dominance of the main stem by LST’ing down . I noticed it was leagues ahead of the rest of the plant because it hung over the side of the pot so since I need to start slowing it’s vertical growth I figure now Is also time to get the rest of the canopy to catch up leaving me to space things accordingly later when/if necessary . Also decided to go ahead and do my 1st & last additional feeding since these girls where planted all I’ve had to do this grow was water when dry & train her to the best of my abilities. Week 6 on the GreenSceene in the books 📚 thanks for stopping by .

Week 02 Introduction This week has been a bit of a struggle. Had my first experience dealing with slime and brown algae. But I think adding enzymes and increasing the beneficial bacteria helped to fix the issue. Also experienced nutrient burn for the first time. My nutrient solution strength got as high as 671 PPMs. So backed it down to about 430 PPMs during the end-of-week reservoir change. Also cut Hydroguard (expired?), CaliMagic, and Superthrive from this grow. Now she's still stunted, but is starting to recover. Hopefully... [START OF WEEK 02] Day 01 - Monday - 08/10/20 - Total Days: 008 ----------------------------------- - [0830]: Still looks a bit yellow. But getting larger! --- Solution strength was at 248 PPMs. --- Just topped off with a bit of distilled water --- PH went up to 6.1 so PH'd down to 5.8 --- Ran the top feed for a bit to wet the hydroton. - [2130]: She's looking even more yellow now. --- Even the new leaves are really yellow. --- Decided to add more base nutes. --- Added 4ml of Sensi A --- Added 4ml of Sensi B --- Now at about --- Solution Strength went up to 304 PPM. --- PH was at 6, --- Also raised the water level a bit more. --- I really hope it's a nutrient deficiency and not an over-watering issue. --- Please live! Day 02 - Tuesday - 08/11/20 - Total Days: 009 ----------------------------------- - [0830]: Looking less droopy. But still yellow. --- Decided to do a reservoir change. --- 4.5gal of distilled water --- 8ml of Sensi Grow A --- 8ml of Sensi Grow B --- 8ml of Voodoo Juice --- 4ml of B-52 --- 4ml of CaliMagic --- 4ml of Hydroguard --- 4 drops of Superthrive --- PH'd down to 5.8 --- Solution strength: 302 PPM --- Since I didn't want to risk over-watering the rockwool, I did not to do a top feed, may do one tonight after the sleep cycle (lights are still set to go off at 10am and back on at 4pm). --- As I was doing a reservoir change, I noticed that the tubes and bucket were a bit slimy. Didn't really stink or anything. Is this normal? Maybe due to the Hydroguard and Voodoo Juice? --- I hope she gets better soon! - [2215]: Getting larger! But still yellow. --- Also can see a root growing through the net pot! Tiny little thing but it's there! Will have to start dropping water once more appear. --- After looking over a few DWC diaries using AN nutes, seems like my solution strength is still too low. Most around this stage average around 500 PPMs with about 4ml per gallon. --- Top fed for a minute to wet the top of the hydroton. --- Solution strength went down to 263 PPM. --- Added 8ml more of Sensi A --- Added 8ml more of Sensi B --- Now base nutes are at 16ml for 4.5 gal of water. --- PPMs went up to 411. --- Still lower than 500 PPMs, but that's okay. --- PH was at 6. Not sure if I should adjust PH using AN nutes. So going to not ajdust unless PH goes above 6.1. Day 03 - Wednesday - 08/12/20 - Total Days: 010 ----------------------------------- - [0830]: Looks like more root growth! --- She still looks yellow. But leaves are perky. --- PH was at 6.2 so lowered down to 5.8. --- Solution strength was at 394 PPMs --- Since roots are still growing, I decide to lower the water level a bit. Now water level is a bit below the net pot. - [2200]: Root is getting longer! --- Slime is returning. Not sure why. --- Solution strength: 357 PPMs --- PH: 6.1 --- Decided to add an enzyme along with more beneficial bacteria to hopefully cure the slime. --- Added 16ml of Sensizym --- Added 5ml of Hydroguard --- Added 8ml of Voodoo Juice --- New solution strength: 430 PPMs --- New PH: 6.1 --- Top fed for a minute. --- This slimy residue is really freaking me out! I hope the added enzymes will help eliminate the slime. --- Going to do a full reservoir sterilization/nutrient change over the weekend. Please survive until then girl! Day 04 - Thursday - 08/13/20 - Total Days: 011 ----------------------------------- - [0930]: I think the enzymes worked! There is little to no slime now! --- Although I think the root was also dissolved in the process. It's now just a little stub sticking just below the net pot. The enzyme is also suppose to dissolved dead roots too but guess that root was too weak? Not sure what happened there. But I think the slime is cured! --- Solution strength went down to 395 PPMs. Decided to add more base nutrients. --- Added 8ml of Sensi A --- Added 8ml of Sensi B --- Added 4ml of B-52 --- Solution strength went up to 598 PPMs. --- PH was at 6.1 --- As long as the PH is between 5.5 and 6.3, I'm not going to use PH Up or Down. But still will monitor PH levels. - [1715]: Checked up on her after her nap and shes looking alive but stunted. But no slime! --- Decided to add some sugars to help maintain the enzymes and beneficial bacteria. --- Solution strength was at 587 PPMs. --- PH was at 6.2 --- Added 8ml of Bud Candy --- Solution strength went up to 671 PPMs. --- PH also went up to 6.3 --- Lowered water level a bit to hopefully promote more root growth. --- Top fed for a minute. --- She is getting bigger, but still looks yellow. Day 05 - Friday - 08/14/20 - Total Days: 012 ----------------------------------- - [0915]: Leaves look a little burnt on the tips. Still no slime. --- Going to back off on the PPMs to hopefully fix the nutrient toxicity. --- Solution strength was at 632 PPMs --- PH was at 6.3 --- Added/Removed water until PPMs were at 536. --- PH was still at 6.3 --- Raised water up a bit, then top fed for a minute. - [2200]: Looking better. Still yellow though. --- Solution strength: 514 PPMs --- PH: 6.5 --- Added PH down. --- New solution strength: 518 PPMs --- New PH: 5.2 --- PH was kind of high. So decided to PH down. Had to use quite a bit to bring it down. But eventually got it to drop down to 5.3. A little low, but so far, the PH has always gone up so wanted to start it really low. --- Ran the top feed for a minute to re-saturate the top of the hydroton. Day 06 - Saturday - 08/15/20 - Total Days: 013 ----------------------------------- - [0830]: She looks stunted. leaves are still yellow. --- Solution strength: 514 PPMs --- PH: 5.6 --- Top fed for a minute. --- Not sure what to do at this point. Going to do another reservoir change soon. Hoping she lives until then. - [2145]: Looking pretty much the same. --- A bit of slime has returned. Since I've been diluting the nutrient solution, the enzyme ratio has dropped. So I guess this is expected. --- Also noticed that slime build up was on the air-stones. Never really checked the stones so not sure how long that has been there. Looks brown too, which can't be good. --- Going to do an H202 sterilization of the system (minus the net pot) tomorrow so did not add anything. --- Lowered the water level to below the net pot. --- Then top fed for a minute. Day 07 - Sunday - 08/16/20 - Total Days: 014 ----------------------------------- - [0900]: STERILIZATION DAY! --- Decided to skip today's night cycle for a deep clean. --- I'm hoping that skipping a night cycle won't be too stressful. --- Temporarily moved her to a new bucket. --- Used about a half of gallon of straight distilled water to flush out the net pot. --- Drained the system and wiped up all the brown sludge. Also removed the air stones and cleaned up all the goo. --- Filled the system with 2 gallons of distilled water and at least 30ml of food grade hydrogen peroxide (35% strength). --- Yea its a lot, but since I'm not going to sterilize the system overnight, I went with a really high doseage. Basically I kept adding until I started to see little bubbles start to form. --- Then I ran the water pump. - [0915]: Boiled the air stones in tap water for about 10 min. Then reconnected them to the airlines. --- I let the air stones and hose soak in the sterilization solution for about 5 minutes so the peroxide can absorb into the stones. Then ran the air stone to help mix and agitate the solution. --- Occasionally, I'd repeat the process of turning the air pump off for a few minutes to let the stones soak. - [1000]: Covered the top two row of holes of the net pot with some aluminum tape. Hopefully this solves the light leaking in through the hydroton. - [1200]: Used an old toothbrush and scrubbed the pump clean. Then drained the system. --- Refilled with more distilled water and peroxide. Again added a lot. --- Ran system again to sterilize even more. - [1530]: Drained the system and refilled with distilled water to rinse. --- Ran the system for a few minutes, then drained and rinsed again. --- Did this a few more times over about an hour. - [1630]: Added 4 gallons of fresh distilled water. --- Added 15ml Sensi Grow A --- Added 15ml Sensi Grow B --- Added 12ml of B-52 --- Added 16ml of Voodoo Juice --- Added 16ml Sensizym --- Solution strength was at 430 PPMs --- PH was at 5.6 --- I was going to add Hydroguard, but I think the bottle is expired. Since 2017 or 2018!? I think Hydroguard is good for up to 2 years from the date on the bottle or 6 months after opening. So not going to use from now on. I wonder if this could have contributed to the slime build up. --- Also removed the CaliMagic and Superthrive too. Going to only use AN products to eliminate the chance of incompatible nutrients. --- Placed her back in the bucket and top fed for a minute. --- Here's hoping for the best. Going to let it ride until morning! Please feel better! [END OF WEEK 02]

planted in July it started to grow uniformly with very fragrant leaves of Sicilian lemon peel you can smell the citrus notes in the pre-flowering leaves I enjoyed its very fresh zkitty phenotype which tended between the sour green apple and the gaseous lemon the variety is particularly susceptible at high temperatures and is the only one that on the fourth day without water this summer literally twisted its leaves on itself variety that had some problems with leaf problems but the yield was excellent with rock hard buds! THE AMAZING TERPENES THE BEST IN THE WHOLE GARDEN. GROWING SCENTS: green apple organic citrus peel TERPENES: THOSE WHO SMOKE THEM ARE STUNNING.🙌🙌😍 the complexity and flavor of zsrawberry begins with a very deep hit of hearty heavy spicy smoke which soon reveals itself as a fresh lemon zest surrounded by nutmeg spice followed immediately by the sweetness and fruit of zkitty swallowed the smoke closes with a note of completeness of the three perfectly balanced flavors that tends towards diesel, strawberry spice and a dream of blackberries and redcurrant fruit salad, lemon peel with an acid finish and a round spice of aged jam😵😭😭😍

Día 71 (24/03) La planta lo ha pasado mal tras 2 días sin riego (casi 3 ciclos completos de 12 horas de luz sin riego...) A lo que se suma altas temperaturas en mi zona, han tenido 28 ºC durante las horas de luz... Las hojas inferiores se encuentran caidas y alguna quebradiza... Le hago un riego con 750 ml H2O EC 0,45 a ver si se recuperan.... Día 72 (25/03) Riego con 250 ml H2O EC 0,45 Elimino las hojas quebradizas que no se van a recuperar después de la "sequia" que han pasado Se encuentra en buen estado general y formando nuevos pistilos! 😍💥 Día 73 (26/03) Riego con 250 ml H2O EC 0,45 Día 74 (27/03) Riego con 500 ml H2O EC 0,45 con BIO PK 5-8 a 10 ml / L Día 75 (28/03) Riego con 400 ml H2O EC 0,45 Día 76 (29/03) Riego con 500 ml H2O EC 0,45 Día 77 (30/03) Riego con 400 ml H2O EC 0,45 🚀 Khalifa Genetics - https://khalifagenetics.com/product/lemon-blanco-v3/ @khalifa.genetics 💦 BioTabs 15% DISCOUNT code "GDBT420" biotabs.nl/en/shop/ @biotabs_official 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE @promixmitch @promixgrowers_unfiltered 💡2 x Mars Hydro FC1500 EVO Led Grow Light (2024 NEW FC 1500-EVO Samsung LM301H 150W LED) - https://marshydro.eu/products/fc1500-evo-led-grow-lights/ - https://www.amazon.de/dp/B0CSSGN5D8?ref=myi_title_dp

Luego de una etapa vegetativa con muchos problemas se agrega un accidente que me deja sin las copas de dos de los ejemplares. En esta etapa casi llegando al final de vegetativo, voy aprovechar los últimos días de calor del verano que se va en unos días para que crezcan lo más posible. Siempre estimulando y agregando nutrientes para su salud.

Growing nicely, I'm hopeful she will be colorful later on in her life. She will be going outside, I have wind blowing to strengthen the stems up to prepare for mother nature.

68h light off

She is getting fat! What a great plant she is - again, little to no deficiencies, other than some light burn but I could not complain to have such strong genetics for my first ever grow! Trichomes are not too cloudy, so still a while to go but what an AUTO!

Week 4 I had some problem with humidity go down and up many time will see where will end up thanks everyone

I’m loving week 7 of flower for the plants on 11/28

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

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