My cat died this morning !! It was my painkiller with weed when the disease was breaking my balls !!! His name was RICK and his Sister named MORTIE. Rick was the sweetest cat of the world. apparently he had an autoimmune disease which took him away. he was 4 years old. RIP my RICK

Day late on the update, pics/vids were taken on time tho just been a crazy week of harvests and trimming and keeping up with these. I linked up with Rain Science Grow Bags on Instagram and got them to offer all my followers and friends a discount of 10% off entire order from their site with the code ' bangdang ' so if anyone is in the market for a pot upgrade use that code. I got them in the mail 3 days after I ordered. Reason I went with Rain Science is because they offer identical air flow for rapid growth as the radicle bags, just using a different material and a tighter knit so water doesnt flood out the sides during feeds and when you pick these up when the coco is dry, it wont fly all over the tent like with the radicles. They're the optimal bag for autoflowers especially. These 2 are doing great should get interesting in the next few weeks. I actually ran 3 of these in 1 gallon pots a few months ago and 2 atleast came out pretty good so the bus shots are from the 2nd pheno of those.

Back to the 1000 wt as temps here are modest for July atm. Still 1 plant not flowering like the other 2. I will be patient as it’s overall structure looks amazing. Slowly easing into the process not as much anxiety about every little thing. Changed my light out for my lower power 600 wt led instead of the 1000wt TMLAPY. Maybe not the right move i really don't know what to do b/c i have tip burn and my tent gets a bit hot in the low 80s when its in the 90s here in Illinois. I have an makeshift cooler w fan and some fans and my exhaust fan system which im pretty sure is under powered and overall and bad beginner purchase. I will upgrade as soon as i can financially afford to. Also had flooding issues in basement which is always an adventure, i eventually need a better way to get my grow tent up off the ground b/c water does get in there sometimes. Doing everything in my power to avoid mold and have fans and filters and cleaning, but again this may have been a beginner mistake and i may have to find a new home for the tent for the next time. Also I definitely had been under watering the girls and came close to one of them dying and have to remove some yellow leaves at bottom, they all look stable now. 1 out of 3 plants has a darker color and looks fuller and healthier w less tip burn but it is shorter only has a couple of hairs where the other 2 are going full on into flowering with so many hairs and growing much taller. Possibly stunted the growth... Anyway the word of the day for this week is TIP BURN. I will submit a question to follow. i recently uploaded a video that should show all 3 plants w the tip burn.

OMG the smells are becoming so intense, from lemon to the sweet caramel mix with chilies and a touch of pine trees, i think i cant put in words what im smelling and were is taking my mind, but i can tel this, what an amazing combination of fragrances that are flying around The tricomes are shouting up as they become fatter and frostier, all cristal clear so far, i think i still have 3 mb 4 more weeks to harvest, lets see 😜 Just calculate my VPD and it’s 0.98 kPa need to increase this up to 1.2 for now s i’m moving my ligth up a bit and see if it works 🙏🤓🙏 Thank you all for following, comment, like and all 🙏 100 likes 😅🙏 🙌🙌🙌🙌🙌🙌🙌🙌 ❤️❤️❤️❤️ Loving this LED Tec 😍 Girls: 1-BlueBerry 2-Alaskan Purple 3-Poyote Gorilla 4-Hindu Kush 5-Whitw Mango 6-Super Glue 7-Badazz Cookies 8-S.A.D. tent -8x8 / 2.4x2.4 but i'm only using 1/2 so 4x4 / 1.2x1.2 Led - Lumatek 465w Compact Pro at 100% All i Grow is medicine for myself, Stay safe, stay tuned and B Happy Peace out D

Vamos familia tercera semana de floración de estás apollo black cherry de SeedStockers. Que ganas tengo de ver el progreso de esta variedad, las plantas están sanas, se ven con buen color. La cantidad de agua cada 48h entre riegos. Esta semana añadimos nutrientes de la gama Agrobeta. Las plantas están bastante bien ya llevan una semana en 12 horas de luz 12 oscuridad. Estas próximas semanas veremos como avanzan. Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Hasta aquí todo, Buenos humos 💨💨

This grill went really well. In growing three different strains it did pose a bit of a problem later in flower due to differing light conditions needed by the three different plants. The Cali Kush being more Indica required less light and the jack Herer needed a bit more to finish nicely. This caused some light stress to the Cali Kush which caused late flower Hermes but didn’t cause any long-term problems like seeds or burn. All in all I’m happy with 600 g from a 4 x 4 tent and a 480 W light. This is especially true when you add in the Gaia Green and how easy it makes growing.

Pretty satisfied with this week as it was a very easy one. Not too hard to just balance some h2o and give the girls a drink. I’ve noticed they are slowing down a little bit on water though. Still about 80% trichomes are cloudy. About 40-50% of the hairs have gone brown. I gotta say these girls haven’t produced a lot of stigmas and I’m perfectly ok with that. The terpenes and THC coverage is perfect for what I’m looking for out of this strain. Think I might pay for the bud to be tested lol

"it is going lightly, I have applied a spray of cal-mag leaf, it really is not possible to compare time with soil so, avoiding high watering so as not to leave the soil moist for a long time to wait for nature to do its part, fed with water this week , this soil contains organic mixtures and has a time of use maybe in the future I can think of introducing some more legal organics of strong nutritional weight ....

Update in the video😁.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Growing and flowering

Day 63: Watered the plants 0.5L with nuts, 4L total, 919 ppm, 1955 us/cm, PH 6.4 Day 65: Watered the plants 0.5L with nuts, 4L total, 780 ppm, 1661 us/cm, PH 6.4 Day 67: Watered the plants 0.5L with nuts, 4L total, 729 ppm, 1563 us/cm, PH 6.4 Day 69: Watered the plants 0.5L with nuts, 4L total, 905 ppm, 1925 us/cm, PH 6.4 Next irrigation's without nuts, flushing next week 1 plant with foxtails (good ones)(small too)

23Aug This week I'm ramping up Top Max as per the feed schedule. 26Aug Spotting some calmag looking brown rusty spot on the top leaves of one of the plants (shortest one, furthest from the fans). Discovered after watering, but still fed it with 1L water pH 6.5 with 1.2mL/L calmag. I lowered the lights recently but they are not showing signs of light or heat stress. 29Aug Watering all with deficiency-strength calmag today to get ahead with the three others, just in case. Buds seem to have fattened a lot in 3 days. One girl is still showign calmag deficiency.

_____----*Jan 13th added photos*----_____ _-** Jan16th added photos **-_ She's still chugging along entering week 3 now.. Just gave alil recharge so far , besides having some water issues here (tap got funky funky) but had some rv water filters plopped onto the tap and all is in range ec/ph wise now. Issue averted atleast i caught it, got in the habit of checking PPM of the tap everytime just to be sure (I aerate 5gal of tap water as I use it to mix whicheve stuff I maybe using aswell. That's about the only major thingy that really happened. I am seeing some leaf variegation I hope it's nothing more serious havnt had any tmv before so I'll be keeping a tight eye. Fingers crossed tho, I do have a second started that was gonna ne ran without plagron but if she's sick I got a back up. Hoping it's nothing tho, 🙏 Until the next update , I'll throw more photos up as the week gets going. Thanks again for checking in, best of luck to anyone that's involved in this contest !! Been fun watching all ur plants get going !

Added the first screen into the tent. Decided to kick Big Mumma out because she just wasn't growing any taller and the other plants were really over growing her. For the new setup I used kitty litter trays and mesh to sit the plants on - each plant gets its own tray so I can track runoff EC individually and I cut little flaps in the mesh so I can wet vac the runoff. With this setup I shouldn't need to move the plants out of the tent until harvest. For the scrog I just tied some builders rope around the poles of the tent and tied the trellis netting off with training wire. I've been manipulating the growth just to try and fill out the whole screen, I also gave them a good haircut because I plan on flipping them soon, don't want them to outgrow the tent.

topped August 28th 2018