Welcome to my Lemon Orange Diary sponsored by Green House Seeds. Day 57-64 Week 9 She grew very well. Really healthy through pre flower & very happy to be high stressed. Kept up with Atami Bloom Stim until mid wk 9. And then I started pushing PK booster from plagron and made sure silica boost was given for the stress. Which helped the nodes not break as I HST'ed after pre flower, so there was no growth in the nodes to spring them back up. So the stems walls needed to be strong. And they were. Some naturally did rise towards the light. But not enough to point to rapid but stiffness back into the nodes upward growth, so it's important to have strong membrane walls. After pre fower was over, I cleaned her up also. Took 70% of everything, (defoliation that was done over 3 days.)The other 30% was removed during ripening as it had loads of (hat leafs) leafs surrounding the tops of the main buds blocking light to buds below. Plant has moved great through pre flowering. Very uneven nodes. And where the rest of where my plants where I needed this smaller. So early into week 9 I lollipopped were I knew the HST bend be everywhere below it was stripped. 90% of the nodes where HST'ed. Maintenance Summary 1:Start of wk 9.i lollipopped her, Everything below the planned HST points was removed 2:Defoliations were done in 3 stages. Removing big fan leafs once pre fower was over..I left more than I normally would as HST was in the same wk so I didn't wanna stress too much 3: HST'ed was applied and to make sure she kept a level canopy her nodes were tied down. 4: made sure the soil didn't dry up and was given silica to protect the nodes main membrane as the nodes would be moving for a wk r 2 until it stiffened during fattening. Thanks to my sponsor from Green House Seeds for this amazing strain. Looking forward to seen what other gems I get from my other GHS strains for this crop. Sorry for delays in diaries. I'm trying to catch up. All sponsored diaries are my priority. Thank you for your patience (sponsors)

Week 10 Flower After 4 whole weeks of flush this girl has finally met the scissors ✂️ It took her quite some time to fade out, but after cutting down the most faded half of the plant that was closest to the light on Day 65, and then the rest on Day 70 it was long enough. I'm very impressed with how fat and chunky this girl has become. I wasn't expecting quite this size from first look at her thinner stems and branchy structure earlier in flower, but her buds have swelled up nonetheless and she's stacked well. I always cut my girls down around half an hour (or a bit more) before the lights are due to turn on. This prevents her from starting any chlorophyll production in order to maintain the best flavour and burn. Only a few fans were taken off before being left to hang dry as she's absolutely covered in trichomes! Don't throw away your sugar trim (leaves with trichomes) as this can later be used for dry sift, which can then be pressed for some dank rosin. Harvest update coming soon! Thanks for following and happy growing! 🐺

Day 92. Almost there. Will flush next week.

Update 14 days after going into soil Looking healthy but abit slower than expected but my first time doing autos so not to sure🤔 i started them on small amount of nutrients today let's see how they do🤞 lights set to 25 percent power at 24" above canopy

There is a timelapse of this past week! This plant, the blueberry, is in the background. Early this week I transplanted the rock wool cube into my DWC system. I pH'd the water, but otherwise did not provide nutes for a few days. The grow tent is now on an 18hr light period. Sometime in the middle of this past week, this blueberry started wilting! In response, I provided the nutrients listed above, with the addition of 1 gallon of (hard) tap water to provide calcium. Unlike my GSC, the nutrients did not fix the wilting, but the wilting did go away after 2-3 days. I had some humidity troubles, so I resorted to flooding the bottom of my tent. This does seem to help maintain humidity around 50% RH. This plant is growing asymmetrically! It's first set of "real leaves" is not even. It will be interesting to see how this pans out.

Had some humidity issues been watering with spring water until I get something to filter the tap water Jan.8th amnesia haze 1 got bit taller so I did some more LST

Week 15: We are heading into flower! I noticed the G3000 instructions say for early flower to set the plants to an 18/6 timeframe, bring the light to 12" distance from the canopy, and increase the amount of light to 60%.. I'm in the business of following instructions, so I did this.. and hope I notice some stretching... I will say I noticed some browning on the edge of some leaves... but I also gave the plants two rounds of the Terp Tea and their first round of flower fertilizer on (10/18) so that might be contributing... Will be keeping an eye out for light burn and will be working to stay on top of watering. At least 2 times a week... with just under a gallon of water per feeding per plant.

This week I used #greenhousefeeding bio enhancer !! Drink, my daughter😍😍🌱🌱 I will try to maintain the temperature difference between day and night😌

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

My poor girls... the calcium toxitity got worse before it got better. Even after the flush, the problem continued for a few more days so the leaves are worse for wear. I'm so upset about this. Ughh. I've had issues with every grow when it came to flowering. Unsure why. Last time I had pH issues, this time a calcium toxicity that causes a lock out of I believe at least phosphorus. They are on the mend now even though the damage has been done to the leaves. Their buds have already fattened so much more since the actual flush last week. The leaves are just terrible though. At this point I'm just hoping to come out with an ok enough yield so that this whole thing won't have been a waste. I learned a lesson with this grow... the water here for this grow is higher in calcium than the city we lived in with my first 2 grows. Next grow, using calmag VERY sparingly. Hopefully my 4th go will finally be the charm. 😖😕😖😩😫😭😭😭 8/25/22: Well the girls have recovered pretty well. No more lockout or leaves shriveling. The buds are getting fatter and we've got some cloudy trichomes already but they still need another 2 or 3 weeks after being as sick as they were. Both the Granddaddy purps are have some purple coming through... excited about that. I also noticed that the stems on all 3 plants that were super dark red before the flush is now are lightening up. It's pretty amazing how these plants quickly recover. 😍

Well she’s in flush and I’m hoping for a great finish with some beautiful fades! She smells of sweet fruits and a deep smell of dank defiantly an OG dominating! Can’t wait to start her sister blackberry OG got my new tent now thanks to my sponsor! So will have more room to grow my plants properly! Under my 2x TS1000’s

I have been flashing for two weeks now I thought it was ready last week but this planet is slow to harvest I think a week or two it will be ready Left in two days of darkness to encourage finish. Worked on half of the plant

Welcome back growfessors to another episode of growfessor theatre! Week 6F begins for the ladies of the 4x8, they continue to bulk up, frost-up, sticky and they got that good stank! The ladies are hungry! Going from 4 liters of nutes + water per plant, to 6 liters per plant per week (extra feed started on week 5). They get 4 liters at the beginning of the week and then 2 more liters on day 5 of the week. Well that's all for this week, I hope you enjoyed the show growfessors 👽🌳💚

Another solid week with more sun, but the plants got kinda shot up from wind. No damage, but after a whole night of wind the plants were leaning a bunch and looked visibly frightened. Strung up the wedding cake for early training and it’s looking a lot better, CC seems to have mostly straightened itself out. Let me know what you guys think!

No more hermies that I've seen. Still on the lookout. More defoliation Little by little

💩Holy Crap Growmies We Are Back💩 Code Name FBT2311 Well growmies we are at 28 days in and everything is going great 👌 👉So the low stress training been going well 👈 We got some major flowering already 👌 let the stretch begin 💪she's the top of of the lot 💪 Lights being readjusted and chart updated .........👍rain water to be used entire growth👈 👉I used NutriNPK for nutrients for my grows and welcome anyone to give them a try .👈 👉 www.nutrinpk.com 👈 NutriNPK Cal MAG 14-0-14 NutriNPK Grow 28-14-14 NutriNPK Bloom 8-20-30 NutriNPK Bloom Booster 0-52-34 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉THANKS FOR TAKING THE TIME TO GO OVER MY DIARIES 👈

Estupendas, estoy muy contento con los resultados que voy observando, me tienen enamorado con el olor a frutas variadas, la resina que tiene es impresionante brillan como una cucharada de azúcar, y tiene 67 dias. El proveedor dice que con 70 dias pero alguna le queda un poquito mas de de lo recomendado si que otras están muy bien echas pero otras no. llevo toda la semana lavando la raíz con agua.

Attached is a timelapse of this past week! GSC in the foreground. This week has seen some of the most dramatic changes. She's in flower! Its a beautiful sight to see the bud sites develop so quickly. When I realized this plant is in flower, I've focused more of the grow light on her, sacrificing some of the blueberry's light. This is because the light absorbed during flower will be directly put towards the buds. Another change is I've lowered the light to about 12 inches, providing stronger light during flower. I've noticed that to maintain an even canopy, I have to tie down this plant every day. My priority is to maintain and even canopy, allowing each bud site to receive ample light. I am constantly focusing attention towards the main stem of the plant because I don't want the plant to favor it over the other bud sites. After tying down the plant, I take the time to adjust near by leaves to cover any significant gaps I've created in the canopy. I do this to ensure light is not wasted. At the end of the week, my pH dropped considerably to around 5. Early signs of calcium deficiency are showing. Should be under control after nute change. My nutrients this week were balanced for the transition between veg and flower. In the coming weeks I will provide more flowering nutrients.