The Grow Awards 2026 ๐Ÿ†
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@Micogrow
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This one has been quick so far! Germinated in 2 days and popped from the soil just 2 days after that! Was good to see that the early OregonismXL use didnโ€™t hurt her any. Was it beneficial though? Further testing needed. Sheโ€™s going to stay in the starting closet with my summer outdoor plant, until I harvest the current plant in my tent (2-3 weeks).
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Great smell and beautiful buds with black and purple leafs. Has a super gassy funk with a slight fruity pebble smell on the backend.
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@Belverde
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Good morning growers๐Ÿค— End of week five.. Four days ago I transplanted this beautiful DOS SI DOS # 33 into its final 20l geotextile pot๐ŸŒฑ๐ŸŒฒ.. It has direct contact with the roots and I have placed some mycorrhises (MICOSAT F) which will have an action in a span of about five weeks.. Always with the aim of trying to get the best from the cultivated strain I created a potting soil that will potentially increase Brix levels๐Ÿคž.. As a base I used light-mix soil of biobizz in most, more than humus and inert material, coconut and zeolite in addition to the perlite already present in the light-mix.. To this base I then added various ingredients of organic origin, but almost nothing from animals.. My recipe includes alfalfa, kelp, calcium carbonate, gypsum, phosphorite, azomite, zeolite leonardite, basalt, krill powder, and neem soil.. I will not be here to list exactly how much I put of one element and how much of another because it is a test for me too and there is everyone who tries for himself, even because the quantities to be used will vary from strain to strain๐Ÿ™‚.. I have already made such a recipe once, but being lighter with the ingredients.. Fantastic results๐Ÿคฉ.. The exposed plants looked like "more natural", I don't think it's a term correct but I don't know how to say otherwise๐Ÿค”.. Really high quality of flowers, especially as intensity and characteristics of the terpene profile๐Ÿ‹๐Ÿต๏ธ๐ŸŽ๐Ÿ‰๐Ÿ‡๐Ÿ“๐Ÿญ๐Ÿช.. But in words it is difficult to make the idea correctly.. Anyway.. From now until the end I will simply give water, without worrying about correcting even the pH.. The only external additions that I will make will be a little fish emulsion + molasses (fishmix), unsulphured molasses, probably half-flowering banana macerate and maybe I will also make tea, but I will see week by week how to behave.. For now, that's all๐Ÿ™Œ.. Thanks for reading๐Ÿ˜‰.. See you in seven days.. CiaoโœŒ๏ธ
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November 16, day 28, Scrog and irrigation installed. With the net installed it's a little tricky (though possible) to hand water, so automatic watering is a nice quality of life improvement. Growth is very tight, maybe a little better suited to sog techniques. Smell is powerful for it's young age. Earliest signs of flowering have begun. November 20, day 32, added video of irrigation. ๐ŸŒฑ๐Ÿ‘‰ Check out marshydro_aliexpress on Instagram (https://instagram.com/marshydro_aliexpress?utm_medium=copy_link)
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@SkunkyDog
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Hallo zusammen ๐Ÿค™. Habe sie heute geerntet. Wir sehen uns in 3 Wochen mit dem Erntebericht. Rabattcode fรผr den BIOTABS-Webshop https://biotabs.nl/en/shop/ GDBT420, damit erhalten Sie 15 Prozent
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@simps
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What a great plant growing like a perfect bush growing good not a whole lot update I did a whole lot of topping on this plant sheโ€™s taking everything Iโ€™m dishing out as far as training plants doing good happy with the outcome so far should be a good harvest Day4 of week8. Veg is going great she is really going fast itโ€™s not a very big plant by flower time she be ok on yield so far itโ€™s looking good Iโ€™m happy with grown no problems to report
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las plantas siguen con su bloque o carencia pero ya esto se termina en nada , tienen muy buenos olores dulces y flores apretadas me gusta este cultivo y son semillas de granel baratas y con agua dura ,alguna semilla de mas calidad y agua bien controlada de ec pueden salir cosas buenas en 11L
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Gone to 1.3 EC. Flowers developing in length. Got some issues with heat during the week. The weather here is increasingly hot and I don't have active cooling in my home. Tried to keep temps down but with the temps outside the tent at 25+ celsius is hard. They don't seem to like it much. Looks like they have trouble taking up nutrition.
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@Napo89
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Si รจ ripresa alla grande dopo le varie tecniche di potatura e defogliazione ๐Ÿ˜Š forse pronta per la fase di fioritura?
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167 grams for 2 plants. went 9 weeks, Had a bit budrot in one of the phenos at week 10. Very easy to grow, not a big stretcher imo. Maybe double it size. Havee a gassy pheno and a fruity pheno. If you look for a outstanding indica with extra on the taste e and smell and a heavy hitter...search no further
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1/5 - Day 51 / Day 23 flower - Feeding Day Pots were mostly dry on this day, last time they were fed was a little over a week ago on day 43 (12/28). I've done two small waterings since last feed, about a half gallon of water for each of my 5 gallons and about 8oz of water for my bonsais. My plants are drying out within about 48 hours of each small watering. For today's feeding I went to full strength on my nutes. My solution consisted of: - 1 gallon distilled water - 3ml CalMag - 3 tsp of FF Big Bloom (full dose)) - 2 tsp of FF Tiger Bloom (full dose) - pH: 6.9 - TDS: 342ppm - EC: .7 I gave each of my 5 gallons about a half gallon of the fertigated water each which wasn't enough to produce runoff. I gave them each about another 1/4 gallon distilled water to generate enough runoff to test. I tested the runoff of my Z52 which gave me readings of: - pH: 6.3 -TDS: 1600ppm -EC: 3.2 I gave about 12oz of fertigated water to each of my bonsais. My ZB2 runoff posted readings of: - pH: 7.2 - TDS: 594ppm - EC: 1.2 My TDS readings are definitely low compared to the FF Soil Feeding Schedule. The suggested readings for week 7 are an EC of 2.8 - 3.0 and 1960-2100ppm. Not stressing too much over the reading as the girls are looking healthy and happy. Will add another feeding in between waterings if I start to see any nutrient deficiencies but I don't see any cause to do so at the moment. As you can see from the picture on 1/3, the super cropping I did on 1/2 didn't hurt my girls one bit. By the following day, the tops were already bending back toward the lights. This was a big relief. **************************************************** 1/7 - Day 53 / Day 25 Flower Light watering in the morning today using pH'd dechlorinated tap water w/ 4ml of cal mag. Gave about about a 1/4 gallon to each for my 5 gallons and another 8oz each for my bonsais. **************************************************** 1/8 - Day 54 / Day 26 Flower Noticing signs of nutrient issue on the Z51. Surprisingly, no signs of nutrient issues on the Z52 despite using the same solution and roughly the same amount of water during the feeding day on 1/5. Decided to do a heavier watering tonight, using about 3/4 gallon of pH'd dechlorinated tap water. Hoping that dilutes the soil a little bit and pushes the salts toward the bottom. **************************************************** 1/9 - Day 55 / Day 27 Flower Nutrient issues did not appear to get any worse overnight so that is encouraging. Did some minor defoliation on my 5 gallons this morning to remove any of the smaller branches on the lower 3rd of the plant. Haven't completely removed any branches that are below the trellis. Will start pruning more regularly every other day so I don't shock the plants too much.
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@yan402
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๐ŸŒธ๐ŸŠโœจ๐Ÿญ๐ŸŒธ๐ŸŠโœจ๐Ÿญ๐ŸŒธ๐ŸŠโœจ๐Ÿญ๐ŸŒธ Back at it with Kannabia โ€” huge thanks for the NEW EXCLUSIVE Upcoming Kannabia's seed Launch ๐Ÿ™๐Ÿจ๐ŸŠ Grateful for the trust and for everyone following along, Iโ€™ll run her clean and showcase what she can do. Dessert-party goals: berryโ€“citrus sherbet terps, creamy finish, tight stacks, easy trim, zero drama. Veg 24/0, clean manifold + LST, flip on a happy, even canopy. Cocoโ€™s fresh, seed goes in now. ๐Ÿš€ Added on 12.10.2025 ๐ŸŒž Light conditions: Limited direct light for the first 4 weeks โ€” ~4 h/day on weekdays and ~10โ€“11 h on weekends due to tent sharing and work schedule. ๐ŸŒธ๐ŸŠโœจ๐Ÿญ๐ŸŒธ๐ŸŠโœจ๐Ÿญ๐ŸŒธ๐ŸŠโœจ๐Ÿญ๐ŸŒธ ๐Ÿ’ญโ—๐Ÿ’ญโ—๐Ÿ’ญโ—๐Ÿ’ญโ—๐Ÿ’ญโ—๐Ÿ’ญโ—๐Ÿ’ญ โ—Events & thoughts worth notingโ— ๐Ÿ’ญโ—๐Ÿ’ญโ—๐Ÿ’ญโ—๐Ÿ’ญโ—๐Ÿ’ญโ—๐Ÿ’ญโ—๐Ÿ’ญ 10.08.25 GW1 Sowed seed, soaked starter pot with #1 12.08.25 GW1 Seed popped hip hip hurray 28.08.25 VW2 Stopped using GreenBuzzBloom, took it out of the schedule. 01.09.25 VW3 TriPartMicro 10โ†’30ml, TriPart bloom 10โ†’30ml 12.09.25 VW4 Applied nemadodes against thrips and fungus gnats. 20.09.25 VW6 topped and trained for first time, decided against a full manifold as I lost a few weeks already. 26.09.25 VW7 Topped her one more time for 8 mains 27.09.25 VW7 Topped up the pot with perlite instead of clay pebbles this time, just trying things out, TriPartMicro 30โ†’40ml , TriPartBloom 30โ†’20ml 10.10.25 VW9 have her a haircut, umm be trimming the side shoots tomorrow, rest in the video ๐Ÿ“น 12.10.25 VW9 done some extra trimming to clean up a bit and chose two extra side shoots to nurture info mains, so kept the 8 original mains plus 2 rest in the video ๐Ÿ“น 17.10.25 VW9 FfJ/fpj fish mix 30 โ†’ 60 ml 20.10.25 VW10 Feed tweak: added 3 g Calcium Nitrate/ 30 L (โ‰ˆ 15 ppm N + 10 ppm Ca) 24.10.25 VW11 did a defoliation and trimming session ๐Ÿ“น 27.10.25 VW11 Epsom Salt 0 โ†’ 2.5, Calcium Nitrate 3 โ†’ 9 g 01.11.25 VW12 CalMag 60 โ†’ 30ml, TriPartBloom 20 โ†’ 30ml, Magnesium 2.5 โ†’ 3.5g 02.11.25 VW12 Did a cleanup in preparation for flowering, rest in the video ๐Ÿ“ธ 13.12.25 VW13 Did another cleanup in the tent ๐ŸŽฅ, also switched to the FERMAKOR PK Micro schedule ๐ŸŒฑ๐Ÿ’ฆ๐ŸŒฑ๐Ÿ’ฆ๐ŸŒฑ๐Ÿ’ฆ๐ŸŒฑ๐Ÿ’ฆ๐ŸŒฑ๐Ÿ’ฆ๐ŸŒฑ ๐ŸŒฟ Day to day tasks & actions ๐ŸŒฟ ๐ŸŒฑ๐Ÿ’ฆ๐ŸŒฑ๐Ÿ’ฆ๐ŸŒฑ๐Ÿ’ฆ๐ŸŒฑ๐Ÿ’ฆ๐ŸŒฑ๐Ÿ’ฆ๐ŸŒฑ 07.11.25 VW12 โ€“ Fed 5l of #1 โ†’ 2l runoff 08.11.25 VW13 โ€“ Fed 5l of #1 โ†’ 2l runoff 09.11.25 VW13 โ€“ Fed 5l of #1 โ†’ 2l runoff 10.11.25 VW13 โ€“ Fed 5l of #1 โ†’ 2l runoff 11.11.25 VW13 โ€“ Fed 5l of #1 โ†’ 2l runoff 12.11.25 VW13 โ€“ Fed 5l of #1 โ†’ 2l runoff 13.11.25 VW13 โ€“ Fed 5l of FERMAKOR PKโ†’ 2l runoff 14.11.25 VW13 โ€“ Fed 5l of FERMAKOR PK โ†’ 2l runoff (*RUNOFF reused for tomato plants) ๐Ÿ’ง๐Ÿ’ฆ๐Ÿ’ง๐Ÿ’ฆ๐Ÿ’ง๐Ÿ’ฆ๐Ÿ’ง๐Ÿ’ฆ๐Ÿ’ง ๐Ÿ’ง Nutrients in 30 L #1 ๐Ÿ’ง ๐Ÿ’ง๐Ÿ’ฆ๐Ÿ’ง๐Ÿ’ฆ๐Ÿ’ง๐Ÿ’ฆ๐Ÿ’ง๐Ÿ’ฆ๐Ÿ’ง ๐Ÿ’ง TriPart Micro: 10 โ†’ 30 โ†’ 40 ml โ€ƒ= 0.33 โ†’ 1.00 โ†’ 1.33 ml/L ๐Ÿ’ง TriPart Grow: 0 ml โ€ƒ= 0.00 ml/L ๐Ÿ’ง TriPart Bloom: 10 โ†’ 30 โ†’ 20 โ†’ 30 ml โ€ƒ= 0.33 โ†’ 1.00 โ†’ 0.67 โ†’ 1.00 ml/L ๐Ÿ’ง Cal-Mag: 60 โ†’ 30 ml โ€ƒ= 2.00 โ†’ 1.00 ml/L ๐Ÿ’ง Home-made FFJ/FPJ (new batch): 30 โ†’ 60 ml โ€ƒ= 1.00 โ†’ 2.00 ml/L ๐Ÿ’ง Calcium Nitrate (Calcinit): 3 โ†’ 9 g โ€ƒโ‰ˆ 15 โ†’ 45 ppm N + 20 โ†’ 60 ppm Ca ๐Ÿ’ง Magnesium (Epsom Salt / MgSOโ‚„ยท7Hโ‚‚O): 0 โ†’ 2.5 โ†’ 3.5 g โ€ƒโ‰ˆ 0 โ†’ 8โ€“9 โ†’ 11โ€“12 ppm Mg ๐Ÿ’ง pH Down: Citric acid (BuxXtrade) โ€” adjust to ~ pH 5.8 โ€“ 6.0 ๐Ÿ“ฆ TOTAL: โ‰ˆ 110 โ†’ 180 โ†’ 170 โ†’ 190 ml liquids โ€ƒ+ 3 โ†’ 11.5 โ†’ 12.5 g solids per 30 L โ€ƒโ‰ˆ 5.9 โ†’ 6.4 ml/g active mix per L ๐Ÿ’ง๐Ÿ’ฆ๐Ÿ’ง๐Ÿ’ฆ๐Ÿ’ง๐Ÿ’ฆ๐Ÿ’ง๐Ÿ’ฆ๐Ÿ’ง โš™๏ธโœ‚๏ธโš™๏ธโœ‚๏ธโš™๏ธโœ‚๏ธโš™๏ธโœ‚๏ธโš™๏ธ โœ‚๏ธ Tools & equipment โœ‚๏ธ โš™๏ธโœ‚๏ธโš™๏ธโœ‚๏ธโš™๏ธโœ‚๏ธโš™๏ธโœ‚๏ธโš™๏ธ โœ‚๏ธ 2ร— MarsHydro SP3000 โš™๏ธ MarsHydro 150mm ACF Ventilator โœ‚๏ธ Trotec dehumidifier (big unit) โš™๏ธ Mini no-name dehumidifier โœ‚๏ธ Kebab skewers (LST โ€“ stainless) โš™๏ธ Wire + roast skewers (LST assist) โœ‚๏ธ Scissors (HST) โš™๏ธ Vacuum (for spills & cleanup) โœ‚๏ธโš™๏ธโœ‚๏ธโš™๏ธโœ‚๏ธโš™๏ธโš™๏ธโœ‚๏ธโš™๏ธโœ‚๏ธโš™๏ธโœ‚๏ธโš™๏ธ --- ๐ŸŠ๐Ÿง๐ŸŒฌ๏ธ๐Ÿฌ๐ŸŠ๐Ÿง๐ŸŒฌ๏ธ๐Ÿฌ๐ŸŠ๐Ÿง๐ŸŒฌ๏ธ๐Ÿฌ Mystery seed (Kannabia Seeds) โ€” NEW EXCLUSIVE ๐ŸŠ๐Ÿง๐ŸŒฌ๏ธ๐Ÿฌ๐ŸŠ๐Ÿง๐ŸŒฌ๏ธ๐Ÿฌ๐ŸŠ๐Ÿง๐ŸŒฌ๏ธ๐Ÿฌ Species: Hybrid (GF / swift-flowering line) Genetics: TBA (breeder sheet pending) THC: TBA Effect: Euphoric, relaxed, creative (target profile) Flavor: Berryโ€“citrus sherbet, sweet candy, creamy finish Flowering (indoors): ~6โ€“7 weeks target (GF) Resistance: High (aim: no drama) Indoor yield: TBA Outdoor yield: TBA Structure: TBA Notes: Brand-new exclusive from Kannabiaโ€™s GF line โ€” Iโ€™ll update specs when the breeder card drops. Goal is rich sherbet terps (berryโ€“citrus + cream), low leaf-to-calyx, easy trim. Stage harvest stays on the table if tops finish early. YouTube Link: https://youtube.com/-m8h?si=A7x4Zlr2kj-_ga31
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@Marbanya
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2025-04-13 to 2025-04-19 I have started the flush and will be harvesting in the next few days. The aroma on this cultivar at time of harvest is a combination of musky earth and citrus. No nutrients are used the last 10 days.
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@Max1973
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Day 35 - few pics and vids..... everything going along nicely ๐Ÿ‘ This is a pretty big update, so i'll add to it over the next few days, as i finish building it all.... i'm building these 2 x SS haze, the full tent, just finishing up the setup..... trimming them, changing solutions to 12/12 and flower..... changed the exhaust fan and filter setup to the better outside the tent method.... and installed 12v CPU computer fan, lol ("alta9" fits in 4in ducting perfectly), drops wattage on extractor fan.... 10w tops... got a few kg of active carbon and making/replacing the carbon filter .... i'll add afew vids of the new setup in afew days... Day 36 - few pics/vid of new tent setup...... "alta 9" fan :) post tomorrow after i finish trimmin and settin up night mode etc...... and into tomorrow we go, lol ... Day 37.... slowly getting there..... little bit more to go... this should make things abit more interesting..... right tank (short babie) has : 19 lts of tap water @18oC + 150 ml -dutch master one gold FLOWER.... 6.4 PH - 1400 PPM left tank has : 19 lts of tap water @18oC + 50 ml of elements a and 50ml of b and 10 ml of nitro and 20ml of Crystalic (molasses/pot) .... 6.5 PH - 1200 PPM more pics / vids of the finished tent setup.... roots, transfer, etc... Day 37 is alot of vids of the clean and change to flower..... edit - day after build noticed left tank (nutrifield) not enough bubbles..... pulled out the 4 air pump and swapped to the 2 x original oxipumps... much better, also to fix the problem with ph goin high, and bubbles not stirin the mix, was to add another 10ml of crystallic and another 5ml of nitro... then pumped both tanks with ph down and set em to 5.8-6.2 - both plants have fully recovered from the big trim, and bounced back within 12-24hrs, post pics/vid in abit..... Day 39 - made a freaky video... was curious about what the plants would see looking up at the lights... the lights and buzzing of air compressors and bubbling tank water, and dazzling lights turned out pretty cool in vid editor, was in selfie mode.... and final vid of the bounceback from trim :) ๐Ÿ‘๐Ÿ˜Ž
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both of this clones has the same mother and grow together with same nuts water ect The only difference is pot size ( first in 4L smartpot second in a 6) And buds are different donโ€™t know why
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Opened the tent this morning to be hit in the face with some strong dank smelling bud, it's moreso the plant that's a week or two behind at the back The purple plant tho has a mundane flowery smell rather than a gassy one. Added some explode and A+B bloom (dutch pro) this will be last and only feeding of nutes as I feel like big purps will be ready for the flush in a week or two after that feeding. Asides from that everything is stacking up nicely, little to no deficiencies thankfully ( growing in compost) my first run was in Coco and that was a nightmare checking and regulating EC and ppm every feeding. I'm glad I moved from the Coco as it's just a pain in the ass.
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So heres my germination method. Soak/shake seeds in 3% h202 solution for a few minutes and let sit until tiny bubbles form on seed coat. Rinsed in clean rain water. Then placed in HPO's GREEN-SUPREME used as seed soak solution. With a small amount of ANTI-MATTER for enzymes. Soaked 12hrs (mostly I just soak a few hrs) Straight into Gro-Dirt Super Starter soil. Watered seeds in gently with diluted DARK-MATTER solution to get the mycorrhizal species attached as soon as possible. Once they came up, 18-24hrs later, sprayed gently with a mix of GREEN-SUPREME & RHIZO-MOJO Just a small pinch of both in a 1ltr spray bottle. All this was done in a dark warm place on a heat mat. In a humidity dome. https://www.highpoweredorganics.com/ Happy growing friends ๐Ÿ‡ฆ๐Ÿ‡บ๐Ÿ‡ฆ๐Ÿ‡บ๐Ÿ‡ฆ๐Ÿ‡บ
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@CalGonJim
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9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. ๐ŸšจSome GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. ๐ŸšจGSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The โ€œbutter frostingโ€ resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KOโ€™s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is workingโ€”phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100โ€“150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-ฮฒ-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called ฮณ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where ฮณ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor ฮณ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so itโ€™s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.๐Ÿšจ๐Ÿšจ๐Ÿšจ๐Ÿ‘๐Ÿ‘๐Ÿ‘๐Ÿ‘๐Ÿ‘ 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!
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the waterworks come covered in some olie residue and i only did a few rinses. I think it kept the ph high for some time and i did a few more water cycles after complete setup. Everytime getting rit of more oil film. I like the bucketd rinsed the leca pepples in high ph tap water and again it took me some time to get ph down but this time no oil residue :) After a few days i felt i got ph under control so started the canna nutes schedule.