Into the 4th week of flower already. Got a bit stressed from the massive leaf stripping I did at week 3 and slow to start drinking again. Same strains drinking at 1/2 the speed of my previous grow. Lesson learned here to give the plant time to grow into the large pot before flipping to flower.

Day 100 - (yes I know it's late) She is getting near the end now. Loosing a few leaves. Trichomes milky but not amber yet. Pistils still white in places. I added a 500ml Monster can for scale 😂 hope it's allowed............ 😋

Welcome back everyone, As you can see are almost all seedlings growing. Some where still in the soil. Might happend when giving water(seedlings can turn or get deeper), checked it out and they are alive and well. All pictures are from day 4. The humidifier had been added, so I had to adjust the room. I spotted a corner of the room that gets dryer faster. I adjust the fan for now and see what happens. Corners are always hard to get perfect. Sometimes the light, air, watering doesn't come there properly. Seedlings don't need much nutrients, neighter do autoflowers. with normal soil I would recommend to only give water for 1-2 weeks, since it holds nutrients in it as well. But since I'm using Cocos for this grow, I might have to add some basic nutrients in the upcomming week. For this week I gave/give only water turns and Startbooster/rhyzotonic turns. Update day 5: - Lowerd the lights to avoid stretching, they are now at 75cm distance from lamp to pots. I want them at 40-50 cm, but I don't want to rush it. - Added another layer of cocos to avoid the soil from drying up therefore the roots from dying. This will happen because of lowering the lamps down, the soil will get exposed more to heat. As you can see, the humidifier is doing his thing haha. Pictures and videos where taken without the lights + fans on. because of that, it will look like this. Still fun thought. Arround 7 seeds didn't reach the surface yet, lets hope they make there way up soon. Enjoy!

End of week 6 flowering From this week to end ROW only, that will enough for flush the soil. 2 or 3 weeks

Decided to start dark period(24-48hrs) day 85 mostly milky not much amber maybe some amber on the sugar leaves. I wanted to go 90+days with this auto but I needed the space in the tent for my photos and she looks almost ready, would’ve liked to see a little more amber but nevertheless I haven’t grown much at all so don’t have too much preference yet on trichomes. I’ll chop in a day or two and dry in my smaller tent and hopefully it goes well, my first harvest I dried too fast in low humidity and wasn’t that pleased with the end result. This time I’m planning on lowering exhaust speed and to not be so scared of 50+humidity in drying area and raise it to around 60humidity after a few days. Can’t think of anything else to write, but I’ll update after the chop and dry and 🙏 hopefully all goes well.

Jsem nadšen jak kvetení pokračuje. Ještě máme před sebou velký kus cesty. Dnes 25.5. poprvé přidán Green Sensation. Ať to roste přátelé 🍊🍧🌻🌺🌷💐🏵️

Week 19 and it happens to be a rainy week with on and off cloud coverage which was actually beneficial to my plant but the humidity has been up due to the fact I also use a swamp cooler and if it's gonna be this humid for another couple days then I'm worried about mold developing on the buds so i think I'll break out the AC to get rid of some of that moisture in the air and maybe alternate between the two. The AC hasn't been used once the whole grow cycle so far but living in AZ we get random storms from the monsoon season not to mention tons of dust, high winds, flash flooding which could be used to flush my plant because it needs it now if rain builds up around and gets under the green house, j/k lol. I've been thinking about buying lady bugs to get rid of all the white flies, any experience anybody, would that work? After writing the above, I could hear the wind picking up outside and remembered that a storm was coming so I ran outside to prepare and it punched it's way through Phoenix with extremely high velocity hurricane type wind, gusting up to whatever it takes to bring large trees down or make a wooden shed door fly off with a wall of dust to the sky and then finally finishing with heavy rain. I was outside the entire storm from beginning to end, well at least until the wind died down and it was just light showers by then. Even with having my greenhouse tied down I had to hold onto it for dear life or it would have taken off like in the wizard of OZ. Honest to god I was almost pulled off the ground or several times more onto my greenhouse when the tsunami type wind would hit, pounding me and the greenhouse from behind having to pull down while leaning back with all my weight and strength.The whole time I'm screaming for my brother who was inside to come out and hold the greenhouse so I could tie it down better because it was just coming apart trying trying to fly away or be ripped apart as storm hit us I was holding on for Amy's Green Drop's dear life. It was pretty insane and wish I had video of me doing it but just have a video a friend sent of his backyard getting hit so you can imagine that wind hitting my greenhouse. The after math was bad 😭 the wall of the greenhouse was getting hit so hard by the wind making it press inward towards plant it snapped off in the middle one of the lower branches making the two buds on the end half hanging there from a 90 degree angle from branch so I knew there was no way to support it back in place enough to heal itself so I clipped it off and started drying it. One other next to it got bent up pretty bad so I'm trying to LST it back to place. The storm also killed my swamp cooler somehow, I guess it's because it has a lot of electronic components on top that was soaked from the wind the rain came from the side, I know, you'd think a swamp cooler could get wet. Now I'm stuck using only my A/C and a humidifier to control moisture and temp and I'm worried it's gonna be pretty hard only using AC in such a small greenhouse as it takes time to find the right ratio of intake and outtake air but can only open greenhouse to mix in so much hot dry air, so starting it was getting down into the high 60's, and I have to figure something out because I can't let it get that cold in there for the last few weeks. I'm almost ready to call it quits If I can't find an equilibrium with AC and humidifier, and if there are anymore storms on the way. Next time I'm getting a bigger sturdier greenhouse that goes into the ground and has hard clear walls and roof, other than the recent storm and excessive sunlight finding the right shading it's been very beneficial doing the indoor/outdoor combo grow and would recommend it as you can live in any weathered extreme hot/cold climate and still grow outside,

This plant has been full of surprises this week lol. First I found spider mites! Then I found a little powdery mildew 🤬🤬 Well.... now that the bad news is out of the way how about some good news. She is starting to really fatten up. The smell of this girl is fantastic😎 It really smells better by the day!! 🤪 She has been yellowing out a bit quickly as of late so I may need to add a bit more feather meal and kelp meal to her top dressing routine. I may also add some cal-mag to a watering here and there as needed. I have some predatory mites and ladybugs coming soon so the spider mites time is running out🤨. As for the powdery mildew, I will have to spray her every 3-4 days with greencure to keep it from invading my precious flowers. Greencure is an outdoor farmers best bud!! (Pun intended). I’ve been in this battle before. It comes with the territory when growing in a greenhouse outdoors. Sit back and enjoy y’all. I’ll get this lady to the finish line. 😎🍿

I did bend her down and she's doing Great! I think i will Mainline her or so it Like i did with the Fastberry. The topping went good. She recovers from the Stress pretty fast and ist doing a grat Job! Looking forward. Stay tuned 🤙🏽

Grown outdoors no nutrients just tap water (about 1Gal/day) on the days with no rain... usually around noon when I can see them sag and the weather forecast doesn't predict rain for the rest of the day. I add Terpinator (30mL/Gal) when I water manually. 2020 was an exceptionally warm summer in Quebec. Seeded on May 20th Sprouted on May 23rd Kept in nursery (watered with tap water + B1 vitamin (3ml/L) until Transplanted outside June 16th No Technique applied, she grows as she feels the needs.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Gorilla Jealousy F1 is growing great. She had a solution change a few days ago. She also got lst and selective defoliation done today. In a few weeks she will go over to my Spider Farmer tent to flower her. The other plants are not gonna be ready when she is. She should be showing signs of maturity very soon. Everything has been going really well with her. Nothing more to report at this time. Thank you Seedsman, and Spider Farmer. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 https://www.seedsman.com/?a_aid=Mrsour420. This is my affiliate link to seedsman. Thank you Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

Second week of flower. The girls are looking great. Day 10 - Realizing that I may have waited too long to flip to flower. Plants are over-hanging my table.

Note : Jegliches Equipment aufgelistet welches ich nutze findet Ihr in der Germinations Woche !!! 200PPFD 20/4 Day 14: + Düngen + + 0.5L Flaschenwasser (EC 0.36) + + Canna Terrar Vega 2.9ml + + Canna Rhizotonic 2ml + + PH 6.0

love the trichomes on this auto

Tag 21 war es zeit die Triple G umzutopfen und sie in ihr eigenes Grow Zelt umgezogen 😁 Schon an Tag 22 konnte ich die Pflanze bei Nodien toppen 😃