Wow... She's really entered flowering this week! I've had to bend over her main cola so is could receive light as opposed to growing above it 😂 😕 Also she's been stretching like crazy. I have backbuilt quite a few bud sites. We will see in the coming weeks how effective this has been.

Started training

Die Blüten werden immer dicker. Vorallem die Grapefuel Gelato und die Lemon Orange explodieren. Auf den Blättern werden bei allen auch die ersten Trichome sichtbar.

Das obere Drittel wird geerntet, sehen wunderschön aus. Die Qualität wird von Grow zu Grow immer besser. Super Juicy, richtig lecker und einfach wunderschön wie aus dem Lehrbuch. Sind nun am Trocknen danach kommt der Rest ab.

week six flower everything looks great so far :D buds are filling out & terps going crazy!! GorillaGlue 1: smells woody and fresh GorillaGlue 2: smells more woody and less fresh like a tree I water them with 1.5l every 48h the light I use was set to 100% and it hangs 80cm away from the tops

Day 1 of flower

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Very beautiful and with a very pleasant, sweet smell, difficult to say about. Getting fatter and growing the buds more and more. Starting to increase the resin, getting whiter.

moved her to optic4 led

Bastante bonita, buen producción a pesar de que le dio botrytis en la copa principal y tuve que cortar por prevención. Un olor muy original, honestamente me gusto mucho, la considero fácil de cultivar con un plan de nutrientes medios.

She's looking beautiful and she's getting huge now on her 3rd week,I can't wait to transplant her asap. She's gonna be such a beautiful big bush! So excited! Follow me on this adventure to see how I grow my organic medicine! Peace ✌️ 💚

Rqs hulkberry auto 12 weeks since sprout and 8 weeks of budding. i think I'm gonna harvest it this weekend. There was 3.5 pounds wet without the few stems i hung

Bloom 8 day. the most beautiful of plants 🌿

Week5 March 1st day 30 I transplanted one( the one with no broken branch) inti the 7gal today watered and did some LST I really hope it adapts to its new home I’ve never transplanted before I used extreme garden MYCOS hope it works also moved the light up a bit and plan on going 100% once I transplant the other one March 2nd transplanted the other one (broken branch one) my shop didn’t have 3.0😔😔😔😔😔😔😔 so i used DNC Great Lakes water only soil hopefully this works and I mixed some 3.0 I had left from the other pot cross fingers for nice transition to its new home March 5 been three days since transplant can’t really tell if they adapted to there new homes yet some sings of stretching since transplant I’ve never transplanted before and I’m not sure if they need water or not so imma chill let them go for couple more days then I’ll water scared to over water them right now since I transplanted them don’t want to over stress them other one in 3.0 seems little droopy to me compared to the one in Great Lakes but Great Lakes soil seems bit dry sooooo idk I’m going keep an eye on them

Hello everybody!!,I'm very very sad this time guys,a few weeks ago I had some kind of fungal infection because humidity went to high with the rain and I had to take away 3 of this 4 ladies plus the other one I lost at the beginning because they were 5 and it was due also to a fungal infection however she was in seedling stage and I got the evil dumping off,the good news is that I'm enjoying soo much the only one I have,she's in full flowering and she's producing amazing sweet terps,you can't imagine how disappointed I am,I would have loved to have my 5 girls in full flowering now,I'll griw her Photoperiod version no matter what,I'm in love with her suoer floral and sweet aromas,I recomend this strain to everyone,you will enjoy her so much.peace growmies and cannalovers! ❤️💚💛🌱✌️ #LEGALIZEIT!!!!!!

This is the current week which I decide to update from, so I will definitely keep it up as I recovered my account now 😅 This plant taught me never to allow nitrogen deficiency near a flowering plant😔

Die Blüten wachsen. Der PH Wert und ppm sind konstant. Den EC Wert kriege ich leider nicht unter 2,5. Die Pflanze scheint sich trotzdem wohl zu fühlen. ----------------------------- The flowers are growing. The PH value and ppm are constant. Unfortunately, I can't get the EC value below 2.5, but the plant still seems to be doing well.