Habe zum Ende der 13ten Woche nochmal die Trichome unter dem Mikroskop begutachtet und die Pflanze für soweit befunden. Konnte mir hier viel Beratung verschaffen und bin nun guter Dinge dass alles gut wird. Das Licht ist aus und wird ausgebaut, das Zelt auf die Trocknungsphase vorbereitet. Die Pflanzen lasse ich nun noch für 2 oder 3 Tage im Dunkeln bevor ich mit dem Ernten beginne. Die Pflanzen zeigen Mängel da ich bereits seit einer woche am Flushen bin. Ich bin bisher mit den Ergebnissen zufrieden und muss ehrlich sagen: White Label hat mich kein Stück enttäuscht. Jeder Samen keimte. Jede Pflanze war stark und hat mir auch mal den einen oder anderen Fehler verziehen ohne gleich kaputt zu gehen. Ich bin mehr als zufrieden mit der Wahl meiner Samen und hoffe nun die Ernte in ein Paar Tagen gut abschliessen zu können.

Starting to get some nice frosty flowers. The cycling UVB has really made the terps start early. She is getting smelly which is great - a bit behind the others, but so far she is healthy and stretching well. Regular tea and water regimen and weekly nutes. I plan to add another layer of topsoil with nutes next week. Humidity control has been a slight issue. After watering I am typically hitting 80 - 85 rH - so will need to watch this. I could open the grow room door to reduce the rH but then I lose the CO2 buildup. Will stay the course for now.

beautiful girl ill suggest for anyone to grow! the trim was easy as i prepared the harvest and defoliated along the way! i took a different route that will now add to my future grows where i lowered the lights power to 50-60% and lowered the light close to the tops and had my light turning off 2 hours earlier for the last 3 days! and wow oh wow the density the terpenes the THC i can't wait for her to dry, my normal is 5 days! she smelled so good! could especially catch that mint when pulling off the little fan leaves! she has so many tones! will be sure to seek out some more from super sativa seed club! they're impressive! video will be added and updated as soon as its rendered! be sure to check out my Instagram page for daily updates!

Eai familia, sejam todos bem vindos a mais uma semana de cultivo dessa maravilha da FastBuds. Nesta semana notei que as folhas de baixo da banana purple punch estavam amarelando e queimada nas pontas e laterais, então fiz uma rega com mais nutrientes e até agora as folhas que estão crescendo novas já estão com a cor bem melhor. Também fiz uma amarra para controlar o tamanho, e ver se teria ramificações novas já que ainda não tenho um grow e o espaço da sapateira não e grande, até o momento está dando super certo, as meninas deram uma boa engrossada no caule e geraram vários novos galhos. Acredito que na próxima semana já inicia a pré- floração então dei nutrientes pra elas já se prepararem pra iniciar o crescimento das flores. Obrigado a todos que estão acompanhando até aqui espero trazer um resultado incrível!

TEST RUN OF THE ELUFAH UAP-1500 My lemon cherry Runtz is starting to get some decent structure and finally recover. I don’t like how stretchy the nodes are on this pheno also I do t like the thin leaves. I think I probably got a plain Jane pheno. The plant really stinks though. That’s a plus. I’m sure when I flip to flower it will do well.

Hi guys, What's up? Welcome back to Queen Peaky's Flower Gardens These strawberries have a very strong smell....one of the two phenotypes seems to be sativa-dominant.... Very good ... let them cook in the oven for a while longer 👊

OVERDRIVE TIME💪🏽

Esta es mi primer planta que cosechó con éxito, y estoy muy feliz con mi resultado final 😄 obtuve una muy buena cantidad de flores premium con alrededor de 15% de thc (desde mi punto de vista claro 😅) Tuve algunos problemas durante el cultivo, principalmente que deje que se estirara mucho, debi hacerle LST a la punta principal para que no siguiera aumentando la altura y tener más flores del mismo tamaño, las flores más bajas quedaron chicas y les faltó madurar un poco, también tuve problemas de sobrefertilizacion aun a pesar de que seguí las instituciones de los fertilizantes y de sus indicaciones que me dieron los de servicio al cliente, incluso ya teniendo en cuenta eso siempre use la menos de la dosis de la base ya hasta que crecieron más le fui aumentando, igual creo que es muy cierto lo que dicen de que “menos es más” a la próxima empezaré usando muy pocos nutrientes, aparte creo que si hubiera usado unos leds más potentes, las plantas hubieran aprovechado más los nutrientes, pero igual me sirvió de experiencia, pienso cultivar de nuevo esta genética y sacarle más provecho. Muy recomendada 👌🏻😁

First off I want to give a big thank you to Zamnesia and Plagron for the awesome package right before Christmas 🎄.This may be one of the best gifts I will receive this year 😊.I will germinate these 5 seeds on New years and get the New year started off right.Well had a few things to wrap up but finally Jan 21st all 5 and added one extra Zamnesia Runtz bean so I will have a 6 plant auto run in the 5x5. Day 1 Jan/20/2025 the Runtz have sprouted :)

Easy to grow. Fruity spicy very nice stone and made lots of edibles with this trimmings and have to watch out with dosing LOL.

15 Day dry then trimmed using the Trim Bin. Curing with Grove bags, first harvest filled 3, 1/4lb grove bags. *Updating with clone pictures of this pheno in late flower labeled in diary as you look through, If I pop a new seed of this strain Ill start another diary

It’s bern roughly two months these guys have been growing! Lst’d Both but untied the gg because I wasn’t sure she was benefiting from it so I’m going to do it over. These ladies (I hope) will start their flower journey next week when I revive my new flower tent 😎..stay tuned for some epic buds..I hope!!

Hi everyone 😁 Welcome back in another week update! Have to say girls are looking stunning 😍 frost it's almost everywhere. Trichomes on some buds up to 30% amber and progressing by hour. They are gaming weight on buds nicely as well. Aroma OMG I am sensing ripe sweet fruits, a bit woody and gasy. Planning to turn the lights off on Thursday at midnight and harvest by Sunday 😅 Wishing everyone a great week Stay tuned for daily updates Peace and love brothers and sisters ✌️💚 02/10 Day 71 Watered again approx. 2.5l of water and fish shit at 0.4 ml per 1 ltr. Runoff approx. 50-100ml. 04/10 Day 73 Everything going great. More amber trichomes, almost all pistils hairs are brown. These girls are almost ready. The Day it's just around the corner. 05/10 Day 74 Last watering, in the morning approx. 1.5l per pot. At midnight lights out for good. 06/10 Day 75 Uploaded 2 clips from last night. Trichomes are on point. Some buds 50-70% amber and they did gained some weight overnight. Just one more night of darkness and will harvest! 😎 07/10 Day 76 It's done!!! Harvested and trimmed. Soon will return with final update - Harvest 😁 Thank you all for such a great support, likes comments and pm's Peace and love brothers and sisters ✌️💚

The plant stretched a lot this week and the first pistils have appeared. The weather was very sunny and the temperature rised a bit while the humidity went down. It's not the perfect conditions but the plant grew well nevertheless :)

Week 5 already, thats why they call it fastbuds... In no time its harvest time! We had some bugs (trips) running around, i think i took them from outside as i was working on my garden, my outdoor plant is also affected, that happens for being a dirty old man 🤦🤷 but not much we can do now, i tried to clean the leaves a bit and remove some, but i think its better to harvest and clean the room as i dont eant any pesticides on the buds, but for the rest quite ok, we gave aome extra pk and bloom this week so lets see how the plants do next weeks 😁

it's too hot in the tent, which she doesn't like :( well, but there's no other choice, we'll have to end it like this, I think she'll endure and everything will be fine :)

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Biosys insteat micobe tea once a week

Hi everyone!So i had to rearrange the whole thing because i was doing it all wrong.To be clear 3 gorilla glue and two wedding cheesecakes and in the smaller pot a haze that was added later by a friend of mine.The smallest gg was born a week later so that explains the size i guess. cheers!