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Home Improvised Polyploid Induction HIPI

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2093
2 years ago
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4
Outdoor
Room Type
FIMing
weeks 2
Soil
Grow medium
Coco Coir
Grow medium
Other
Grow medium
Grow Conditions
Week 1
Vegetation
3
cm
inch
Height
28
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7+ conditions after
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Commented by
Med_in_Tropic Med_in_Tropic
2 years ago
Summary None of seedlings dipped in colchicine died. And I wonder if I get any polyploid plants at all. People had published on why and how to make polyploid cannabis since 1940. Seemed that the classic way to make polyploid cannabis is to dunk them in a vat of toxic chemical. This may kill some unfortunate seedling. Some, but not all, of the survivors would become mutant with polyploid power. Before you go on and make polyploid, there are pending patent registrations. Do make sure that you don’t copy their method. Stuff that I used: The chemical I used is an extract from autumn crocus call colchicine. Concentrate stuff is available from the internet. However, gout medicine made from colchicine is easy enough to get over the counter. And I bought 150 of 0.6 mg tablets. This caused me under five USD. Concentrated stuff is cheaper, but I would not know what to do with the remaining stuff. Supply and tools used: mini size jam, facial cotton balls, electrical tape, scissor, small glass jars, rubber gloves, seedling handling box, dishwashing detergent, tap water, plant label, permanent marker. Stuff that I should use but did not: Leaf penetrating agent, surfactant, automatic shaker stirrer. What I thought about making polyploid: Polyploid works during the period of rapid growth. Early seedling stage would be better I started making polyploid from day 2 after Golosa seedlings were germinated. The concentration that I aimed for was 0.2%, 2,000 mg/liter. I used 45 CC of solution. Doing some math, this is 150 tablets of colchicine. Took a bit of time to pop all the tablets. The best success rate from university professors seemed to be only half of the plants become polyploid. So, I treated only three plants with mutating poison. Perhaps I would get one polyploid. I still want normal Golosa. And I keep three without the treatment. Not so much as a control but for the flowers. Polyploid seemed to cause root problems in many plants. So, my idea is to immerse only the top part of the seedlings. So I put them upside down with tops pickled in colchicine jars for 24 hours. One thing that I can see is that researchers are rich and can afford to kill off hundreds of seeds. I, on the other hand, paid a small fortune for these seeds. And I want them to survive even regardless of polyploid success or failure. So I think I’ll attempt making polyploid in a way without letting the root touch colchicine. Here are my steps: 1) Stuff cotton balls around the base of three seedling. 2) Tape the base to compress cotton ball a bit and keep the soil from falling and also prevents colchicine from reaching the root. 3) I bought and ate mini size jelly just to get the tiny jar. Real sacrifice since I normally abstain from sweet. This is just to get containers that are small enough. :D :D :D 4) Check immersion level with the jar. See how much solution to put in. Seedling will bend upward from this unnatural upside down pose later. 5) Safety precaution! 6) Always wear gloves. And I use syringe to fill the jar to avoid spilling. Mutating poison scares me. 7) Tape the bottom of the colchicine jar with long piece tape. 8) Put the plant tips inside the jar. And tape it so it won’t come loose too easily when I leave home for work. 9) Let’s sit for 24 hours. The colchicine from tablet settles on bottom of the jar. I may or may not get 0.2% concentration. I did shake the jar once but it is no replacement for lab gentle shaker. May be I ask could ask a taxi driver to keep plants in his cab for a day? This would be like how Norwegian age their aquavit. 10) Carefully unwrap the seedlings from mutating jar. Keeping the main layer tape on. 11) Clean the treated seedling well with water once. Then dip in dishwashing detergent for a minute each. And then rinse the seedling well again. 12) Ever so carefully remove remaining tape then cotton balls. I was happy to see that the cotton balls were not soaked and I don’t think colchicine got in to the root. 13) To be sure that the root part is not damaged by colchicine, I flushed the plants in their little containers with water. The last round, I added weak compost tea mixed with alfalfa wax suspension. 14) Put label on so I won’t mix them up later. 15) Put the seedlings under 9W white-light table lamp at about 20 cm distance. Week 1 so far: Day 3 – no plants died. Untreated plant kept going. There were white deposit on the treated plants. I transplanted the seedlings to 300 cc containers put these in my computer case light boxes. Day 7 – Untreated plants are a taller than treated seedling. Treated plant number T1 is only slightly shorter than normal plants. At this point, it is too early to tell if the treatment works for this one. Plant T2 is shorter and is growing okay. Plant T3 is stunt. It could be from colchicine or could be a runt. However, I chose the healthy plant for colchicine treatment. Let’s see how she develops.
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Grow Questions
Med_in_Tropic
Med_in_Tropicstarted grow question 2 years ago
Would polyploid weed cutting take root and clone as usual? I treated my Golods plant with colchicine. They maybe triploid already. So, I think I have s plant that is either tetraploid or hexaploid. Would these clone at all? Do I need to take extra cuttings?
Solved
Techniques. Other
1 like
Organoman
Organomananswered grow question 2 years ago
First generation plants that have been treated with colchicine are unsafe to smoke. The plant will be no more potent than the original. Clones should be as straight forward as with normal plants. It is only safe to smoke polyploid plants that have been grown from seeds produced by polyploid parents. Polyploidism only affects size/growth and vigor of the plant, but not THC production, sadly. If it did create super resinous plants, they would have been on the market for decades already.
Med_in_Tropic
Med_in_Tropicstarted grow question 2 years ago
What would be a good fertilizing scheme for making robust seeds?
Solved
Feeding. Chemical composition
1 like
Sunstonedgrows
Sunstonedgrowsanswered grow question 2 years ago
You'll want to fertilize close to week 2 or 3 of flower maybe a bit later. As for fertilizer regimen you do need a good amount of phosphorus, but potassium, nitrogen and your micronutrients are very important. Personally I recommend doing a ratio of 2/3 bloom and 1/3veg nutes. Personally I am using Dr. Earth dry amendments, and the mix of 2 scoops of flower and 1 scoop of veg works great for me.
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Shooey
Shooeyweek 1
My mind is blown 💥
Med_in_Tropic
Med_in_Tropic
😀😀😀😀😀 @Shooey, thanks mate. Hope the result will be useful.
GYOweed
GYOweedweek 1
Can u tell if your roots swelled up?
Med_in_Tropic
Med_in_Tropic
👍 good point. thanks @GYOweed, i’ll look at next container chang. I better wait until root fill out a little and see.
homerjgangia
homerjgangiaweek 0
Good luck with your grow mate!💪💪
Legendaryseedthumb
Legendaryseedthumbweek 0
Happy growing my friend! Nice reading your diary. Say hello if you ever visit my profile🌱 Peace & love L.S.T
HighRoller909
HighRoller909week 0
bery interesting good luck man.hope it is safe to use that polyploid solution
sailormoonflowers
sailormoonflowers
@Med_in_Tropic, 😂😂😂 defo keep us posted on that although could use N2O? have a giggle while you work... 👊👊👊🙏
Med_in_Tropic
Med_in_Tropic
@HighRoller909, just did the colchicine dip. No extra fingers on my hands yet. But it is still early.
Shooey
Shooeyweek 0
Incredible detail Med, nothing is left to chance! I’m impressed with delicious seeds 👍
Med_in_Tropic
Med_in_Tropic
Hi @Shooey, thanks. I would love the taste and smell of SBR. But i was going for resistance. And there s one thing about chance factor. Its my fumbling hands 😀😀😀🙏😀😀
Grey_Wolf
Grey_Wolfweek 0
Back in the day some guys over here in Australia were experimenting with colchicine to induce polyploidism they apparently had some good results with the second generation seeds they made from the crosses I have a bottle of the colchicine tablets for gout and been thinking of experimenting one day Good luck mate 👍 https://www.sciencedirect.com/science/article/pii/S0304423818307908
Med_in_Tropic
Med_in_Tropic
Hi @Grey_Wolf, i started on this. It took me 50 tablets for 15 cc solution. Concentration was at 0.2%. And tablets don’t dissolve well. Lab researchers have this automatic stirrer. May be i need to order lab grade colchicine? And yeah, second cross will get you triploid plants that grow better than tetraploids. And i was thinking about your feminized pollen for the next steps.
Cannabeast40
Cannabeast40week 0
Wish you best of luck and happy growing 👊😎
sailormoonflowers
sailormoonflowersweek 0
Hi Med, good luck buddy. going to be very interesting.... the polyploid saga!! SMF 🙏 😎 🙏
sativaman
sativamanweek 9
On a real quick look, T3 looked like a polyploid. This phenotype would be awesome to work with in an indoor setup: they should grow some huge colas and stay compact. Nice project. About the FFT7, are you trying to revert it without reveg her?
sativaman
sativaman
@Med_in_Tropic, thanks. Not easy to find a good male sometimes..
Med_in_Tropic
Med_in_Tropic
@sativaman, Golosa has a big range of phenotype. O don’t recommend it. You will need to send rt out the pheno’s and clone a few rounds to make it worth the cost of seeds.
Med_in_Tropic
Med_in_Tropic
Hi @sativaman, when it is this late, it takes too much work to reveg. She is unlikely to survive. And she won’t do “rodelization “ either. This is my 10tg FFT7 clone. But since i had not pulled her. Since i won’t be using this spot. A few extra puff of silver her way might get me some sack. Less chunky but more potent than White Critical with similar smell.
GYOweed
GYOweedweek 0
Those will be monsters
GYOweed
GYOweed
@Med_in_Tropic, nice you used colcoochi? How much bap u use? 15ppm or less for seeds?
Med_in_Tropic
Med_in_Tropic
@GYOweed, i hope i get an Audrey II.
sativaman
sativamanweek 23
An other nice cannadventure that comes to the end! Bravo for all the work you've done with this project! mmmm...!!! sticky buds !!!!
Med_in_Tropic
Med_in_Tropic
🙏🙏🙏@sativaman,
Shooey
Shooeyweek 18
Always interesting to read your updates mate. The golosa looks brilliant with all that frost at the moment mate! I remember reading about kratom last time you included it in your post. Last week I learned about “betel nut”. I had never heard of this until I was watching the news and I saw a street sign in Papua New Guinea saying “no chewing betel nut”. It reminded me of kratom, and I start to wonder why we never hear of these things much here in AUS, despite having the same climate and conditions as most of the Asian areas they are popular in. Maybe aussies are lazy?? We found weed, then we stopped looking 🤣
Med_in_Tropic
Med_in_Tropic
Hi @Shooey, thanks mate. Beetle nut is a nasty way of consuming nicotine. It is red. So this is much worse than chewing tobacco. Actually a nice sort of palm to prevent soil erosion near a water ditch. Kratom is a construction worker thing. It grows on trees. So, it is very cheap. Thus its not hip. Buck hoe operators, asphalt pavers have kratom habit so they can brave the heat while working. Kratom is also use to rehab opoid addicts. It blocks the same neurob path that make opoids addictive with much lower effects. Seeds germinate well one fliwer costs two-dollar. And a flower will give you a hundred or so seedlings but these grow very slowly during the first year. And each is a tree.
Shooey
Shooeyweek 17
Great news about new legislation! So is it for personal use? How many plants are you allowed to have now?? I really wish we would move on this here in AUS, it's time really. Whip snake is awesome!! He even looks friendly what a beauty
Med_in_Tropic
Med_in_Tropic
@Shooey, as it is, no limit for home use as long as you don’t sell it. We are waiting for the grace period before the bill became a law. No limit allows me to clone. This particular snake is friendly. We leave each other to our own business. My concern is more about my dogs would hurt him. As it is, these fur balls never look up.
GYOweed
GYOweedweek 3
Wow
Med_in_Tropic
Med_in_Tropic
@GYOweed, this one feeds lightly. Will take a while to recover.
GYOweed
GYOweedweek 15
Nice clustering keep the trichome production up!
Shooey
Shooeyweek 11
Happy new year med Hope you had a great holiday mate 🍻
sativaman
sativamanweek 11
Happy new year to you and all your plants!!
DogDoctorOfficial
DogDoctorOfficialweek 2
💚💚💚Nice 💪💪💪 happy Halloweed and keep it up 💚💚 💚 growers love 💚💚💚
Godesskat
Godesskatweek 0
Everything is better with a beg of weed👻 Good luck happy growing😍