This week hasn't really done much, recon it's the week it focuses on root system, hopefully get alot of vegetation over next few weeks.

Girls started to flower about a week ago and so far looks good and healthy to me. I have only 20cm space left above the light so I truly hope the stretch phase is getting to the end :) I feel like every grow I have done so far I thought I switched too early and in the end I worry about the space so my next grow will be experiment 12/12 from seed. I have seen very interesting diaries utilising this method so I can't wait to test it. Not much else to mention, standard 2.5 EC (my starting tap water is 0.4 probably due to chlorine?) ph 6.0 humidity is around 55% and 60% during the night, I will definitely get dehumidifier foe the last month, because mould has been my biggest enemy during my previous grows. temp is getting close to 29 celsius during the day. I just ordered some additional clip fans so it hope fully helps to reduce any possible heat stress. I defoliated quite heavily about a week ago and when I finished and put them back, I was a bit worried if I didn't go too hard on them, however just two days ago it was back to normal. Did little bit of defoliation once again today, got rid of some bottom branches and older fan leaves getting no light at the bottom. Really excited about next few weeks, if you have any advice let me know in the comment section. Thanks for checking and have a great day!

Kalt und feucht die Tage, aber ihr geht's gut im Zelt.

Empezamos con la floración!! Llevamos 4 dias desde el cambio a 12/12 y todo va bien, la temperatura nocturna se mantiene a pesar del frio exterior, me preocupaba que el agua del sisema bajase demasiado de temperatura pero por el momento todo se mantiene estable. Las plantas crecen vigorosas a pesar del deficit de Ca Mg que tuve que corregir la semana pasada 👍

Day 28 Veg Day 1 Flowering: Hi growmies. Here we are at the start of flowering for these happy healthy little ladies from Zamnesia seeds. Girl scout cookies #1 has now had to have pegs to hold her limbs in place as she keeps heading for the light but I want a more squat plant so will keep her lower this way until stretch has about ended. This should keep the main canopy uniform and lower than they want to grow naturally. It is a manageable size so far but is filling the pot out more each day. #2 is a little smaller in general and is also dealing with a repair from a major split on the 5th node bend. She has made sure it survived but I still need to watch her for any pathogens creeping in. Amnesia haze #1 has gone crazy in the last week and her f.i.m has really brushed her out to fill her pot with so many potential mains. She will need a little defol along the way now flowering has begun but inam hoping to keep as many of those fat workhorse fan leaves in the game. #2 has now lost the rectangular look and has also been held down with tent pegs in various locations to promote many mains. She is a nice growing lady with no signs of any issues. #3 was left a more natural pattern but will definitely have to have some stripping out done as she is so full of growth tips, hidden by so many big fan leaves another potential monster in the making in think. Now we are going into the stretch periods , I need to keep on top of the training to best exploit the space I have to use as I am now fitting another Mars light into the fun zone. (Diary will begin soon !!). A lot of activity this week with harvests on other strains and new seeds going in too. I also have 2 100L living organic soil beds cooking for the next run . Be well and sane growmies.

The BIP started fading. The buds are pretty hard and dense. The smell is already Stunning 😮‍💨😵‍💫. Beatiful genetic From Perfect Tree

Hi growers! The tent is geting preaty full with that indicas.In the last week I toped al of them ,and removed the lowes coals.With the coals I removed I try to make clones,and root them with honey directly in soil.not sure what I gonna do with them,I'am just experimenting with them 😀In the coming days I'am gonna do some LST on them,and start to floower them. All of them growin fast.Blue Kush have some wired leaf deformaition from the start,but its growing fast.Two or thre of them have some brown spots on the leafs,but thats becouse I droped some watter drops on them. Sorry for my bad English 😀

Day 51, Its looking good. The leaves are turning yellow and purple and lot of buds are turning blue. Very colorful, you can tell the bag appeal is going to be a 10/10 as its frosting up very nice as well. Only issue I'm having is the buds weight so much their falling everywhere, I'll do my best to tie them down and hold up the branches best as possible with what I have. Tuesday I'll get more stakes. Tomorrow will be my last day feeding MagnifiCal and I'll start giving them a lighter solution right till the end as I don't flush unless my PPM runoff is very high. You can't take nutrients out of plants after its taken it in. Your flushing your medium. I did want to run it to 70 days, but with everything already falling over and the fall colors. Its looking like 65 days. Any questions please don't hesitate. I'll add more pics right after lights out.

Time for drying. Btw drying them like this was a bad idea buds are pressed from one side may be conceded to move them, well I still prefer hanging drying 🤪

Highest center cola is 21" tall and rest of the canopy averages about 10"... LST included

Week 19 of veg!😂😂 would never of thought I’d of took it this long. The big switch to flower starts Monday! The plants been topped around 40 times over the 18 weeks, 18 cuttings that were taken 3 weeks ago. My first attempt at the lollipop technique , nice little defoliation and a good top dress of Dr organics living soil amendments. I have absolutely no idea what’s around the corner when this is flipped😅 but the scrog nets deffo making an appearance😂 keep an eye out for the diary of her little sisters, there already looking 💥… untill next time 👊🏻

“Tropicana cookies purple “Day 105 from seed. Partial harvest at first, then 3 days later completed harvest and starting dry

Everything seems to be going great for them to all be random bag seeds! Temp is staying a little higher than I would like trying to figure out some ways to cool it down may just order some new lights!

The plant kept stretching during this week. I'm a little bit worried as I don't see the flowers touching each other to form nice colas, but she's healthy so I can't complain, it's my first grow after all. Just hoping in fatter buds and not many popcorns. She's drinking a lot of water in these days and the EC was higher when checking it before the daily refill. Don't know why she's eating less, but I lowered it to 1.6 again. I give her what she wants. Keeping an eye on her development and trying to figure out when to add the P-K booster. No particular problems this week, everything is going ok. I gave her another (hopefully not too heavy) defoliation at day 59 so i guess I'm not going to touch her again till harvest.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Welcome to my Pablø Escøbar diary. In this diary: Seeds: sponsored by Ðivine Șeeds Media: Promix HP Nutrients: Advanced Nutrients, Diablo Nutrients. Light and Weather: Şun☀️and Mother Earth.🌎 ___________________________ Feeding: Tue 25Jun: 4L water not pH'd Wed 26Jun: 5L nutrients pH'd 6.5 Thu 27Jun: 4L water not pH'd Fri 28Jun: 4L water not pH'd Sun 30Jun: 6L water not pH'd Mon 01Jul: 4L water not pH'd ___________________________ Weather hasn't been that bad, a cloudy day, a fresh day and night then warm, rain Friday afternoon thru Sunday morning then Sunday afternoon 55 KPH wind gusts 💨 Anti-tilting device seem to hold them down for now😄 ___________________________ Thanks for stopping by, likes and comments are appreciated!👊🏻😎 Keep on growin! Keep on tokin!!! 😙💨💨💨💨💨

HARVEST TIME!!! 👨‍🌾✂️ ALL 5 Banana purple have been chopped trimmed and now curing in grove bags! Drying took around 11 days so perfect. Dry Tent was around 19 - 21 degrees 60% humidity 🍌🍇🥊🔥🔥🔥🔥🔥 Dry Weight Mainline banana purple - 47.82 gram Thai stick looking one bpp - 54.03 gram BIG MOMMA Purple - 49.96 gram 7gallon banana Purple - 50.55gram Tall Banana purple - 61.05gram ( we have a winner 🏆 ) So happy with results. If I hadn't done any mainlining or anything we might have pushed 60gram+ from them all. I probably won't be doing the main line technique on banana purple again and will just let them go mad as it looks like a 7-10 gram difference in untrained and trained plants. Think I stressed them Bit too much on a couple of them maybe light or nutrients Didn't know about foxtailing until this week when i read about , it hapend on both purples hopefully doesn't effect potency that much. Got a big bag of trim after using spiderfarmer Bowl trimmer ✂️ will try make butter or something lol Smell is! Literally blackcurrant fruit juice with a hint of foam sweets 😋 tried some that has cured for 4 weeks omg turns me into a potato instant couch lock straight to sleep 👌 can't wait to grow this again top notch! See how long it lasts haha 💨💨💨💨