welcome to 📅 Day 79 3/6/2021 sorry I'm a day late on the update she is going very good and I'm happy with the development I may start harvest mid week as I have surgery on the 16th and want the cure to have started as I wont have my right hand for 2 weeks update 📅 Day 82 3/9/2021 today starts the 24 hrs of darkness. I put 3 L of tap water in the pot and I'm just going to let is sit like this

La plante grossis et continue de le faire.

i was surprised that she was done on 2/15/25 spidermites had completely taken over one bud so i decided to check trichomes and she seemed ready.

De momento hubo dos que germinaron mal y seran repuestas por otra Gorilla cookies y la otra por una cream caramel auto,fallo tambien una northern,por lo demas todo bien,segundo riego desde germinacion con poquito de calmax y gooooo..Segundo riego de la semana a 50ml x planta aplicando algun nutriente pero sin subir mucho la Ec a 0.69,hasta la proxima semana budyyes

They're all showing nice little white pistols.♀️ The little one def got a little wind burn from my box fan. 💨🍃 I moved it so it wasnt blowing directly on her anymore.🤷‍♂️🏽 Other than that lots of foliage growth and a little stretching over the last two weeks. Looking forward to watching these develop as the weeks progress. 🤓🌺

Stardawg 2 is doing it's thing. Still got some budding to do so another 2-3 weeks I think at least. Partly because I have moved to a 12-12 light cycle now for #1 to try and flower, as well as a couple of photoperiods I have in the tent. I have attempted to supercrop the tops of #1 as it's above my reflector now. Too big lol. About 70-80cm.

1/10 Week 10 Both are in flush and from the looks of the tricones these are the most potent plants we have grown to date. Drinking has really slowed down but keeping the un-PH one gallon daily flush since tap water is cheap. Annie doing very well under the LED lamp, TS-1000 plenty large enough for one plant in flower. This is encouraging for plans to replace the HID with either a TS-2000 or TS-3000(preferred) this spring before the summer heat. Just a few more days....😎 1/11 Bud pics Harvest Belle on 1/13 - Annie 1/16 Just getting them ready. taking off some useless leaves etc... 1/14 Harvesting Belle today, let her dry out one last day. Annie harvest at weeks end. Harvest pics and total numbers at end of week when I take Annie 👉BELLE HARVEST 963 grams wet 👈 Annie got her last watering today, harvest in two days 1/15 Harvesting in about 12 hours, pics and numbers then

Day 142 11/06/25 Wednesday Harvested the Red Gorilla Girl XL today! This run of autos I haven't done any dark period before harvest except, I managed to catch them just as lights were coming on. She has performed amazingly in consideration that she was only in a 2.5L pot. In hindsight, I now know I am able to do a SOG with small auto pots. Making for an exciting next run. Upon harvesting the Red GG XL, The pungent smell of berry, diesel and sweet scents is intoxicating. The buds are dense and compact, with formed calyx's boasting with trichomes. Wet weight on this was 376g of buds. (All wet trim gets dried in hangers and used for making butter later) To my suprise a super easer one to trim, nice size buds with not alot of sugar leaves. She is now hanging drying in a 80x80 tent , with a small fan on low @ netted window entrance, facing down to circulate air from bottom to top. An exhausting fan with carbon filter also runs 2x a day for 1 hour. Again This lasted for 8days before humidity dropped to 49%. I have now put into containers to allow inner moisture to pull through now. After 3-4 more days she will have final trim and placed into Terp Loc Grove Bags to cure. Once curing I shall do a smoke report, dry bud weight, and update journal. Thank you to everyone who followed along for this one, the comments and support! GROWERS LOVE TO APOLLO @ SWEET SEEDS, for allowing us to play with these fun genetics 💚🤜🤛

I think I have a slowdown in flowering, next watering with pk13/14. otherwise my two fans fell hs replacement by one but it's not great.

As you can see, all our plants are very well. Yesterday we gave them fertilizer for the first time. We add 1ml BioGrow, 1ml BioBloom, 1,5ml Heaven, 1ml Top-Max and 1ml Activera on 1 Liter water and gave 250ml of these mixture to every plant. Thanks for your comments 😄

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Mar 31st Week 16 Changed nutrients to straight Dense Bud Compactor 1 gram/ litres @ 900-1000 ppm for 2 days CR # 1 showing signs of root wrought I removed the slimy parts of the roots and will watch closely On April 2nd I will add the rest of the nutrients to the existing solution April 2nd Fresh nutrients today, all nutrients added 8 litres of fresh water + 6 litres of the existing Dense Bud solution @ 1100 ppm CR # 1 is dying due to root wrought I removed most of the roots today. I changed all the water today with this one. I’m hoping to get it to harvest, but things don’t look good. I will probably flush this soon to harvest what I have. CR # 2 is doing fine as far as roots. Buds are smaller on this one but may fill out more with the Dense Bud Compactor. April 6th Switched CR # 1 to flush solution Root wrought was sever and unfortunately 90% of the root mass had to be removed Time to save what I have

Thanks again Short Stuff Tha Shiznit this one should get real nice. For sure. Tha Shiznit is an Auto strain of the highest possible quality. The Urban Dictionary describes the phrase, ‘Tha Shiznit’ as ‘The greatest, the best ever, simply the shit…’ and this autoflowering strain matches up to that description. Tha Shiznit is a complex hybrid of Amnesia kush, Diesel, Master Kush and Jack Herer so you have some serious genetic quality in the mix.  Shiznit produces medium to large, plants which can easily surpass 1m indoor. She has larger internodal spacing with chunky buds forming at each internode. She will generally have a thick central stem and a dominant main cola, large fan leaves and thick clustered buds. This plant is extremely resinous as you would expect for a strain with so much Kush heritage and in a percentage of plants you can expect to see some pink and purple coloration.  Tha Shiznit gives off a classic old school skunky flavour/fruity and some phenotypes express a unique strawberry smell. When smoked you get an intensely strong flavour on the inhale with the fruity aromas coming through on the exhale.  This strain was tested at Spannabis 2014 and recorded THC levels of 20.6% which for an autoflower strain is quite incredible. These high levels of THC mean Tha Shiznit is one of our most potent straisn and produces a powerful cerebral high that keeps you chatty and motivated.  Tha Shiznit is a very straight forward strain which can handle plenty of nutrients and requires little attention. If grown with some care she can easily produce 60g+ per plant. 

:)

A brown leaf revealed the moldy core of the bud. Based on this sign, I harvested most of the plant. otherwise the Plant did grow well and has some terps leaning to the sour, limone side. had to throw away about 8G modly stuff from main colar. Seems to be about 30-40g left without mold.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

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