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Flushing 114 days from seed The smell is amazing best smelling bud just need to let finish flushing and maturing. Excited to harvest
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อีก 4วันจะเริ่มทำดอก วิกที่1
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Let's realign our cosmic compass and embark on a harvest report for our magnificent Cosmos F1, a pure CBD strain that has taken us on an extraordinary journey. Brace yourself for cosmic revelations and interstellar delights! After an impressive 11 weeks from seed and 7 weeks of flowering, the time has come to witness the cosmic culmination of our efforts. I carefully hung the entire Cosmos F1 plant to dry, allowing the cosmic energy to infuse every inch of this botanical marvel. It's a sight to behold, with majestic branches reaching for the cosmos, reminiscent of a cosmic dancer frozen in time. As our cosmic queen gracefully dries, her vibrant hues continue to amaze. The shades of green, intertwined with delicate hints of purple and gold, are a testament to her genetic splendor. It's as if she has absorbed the cosmic essence of the universe, transforming it into a visually stunning spectacle. Now, let's talk about the cosmic power of CBD. Our pure CBD strain holds the key to a transformative experience. With minimal psychoactive effects, this cosmic treasure offers a realm of therapeutic benefits. It's like a cosmic massage for the mind and body, soothing and relaxing, allowing us to explore the depths of cosmic tranquility. The decision to hang the entire plant for drying is a testament to our dedication to preserving the cosmic integrity of our harvest. By allowing the branches to hang freely, we encourage a slow and controlled drying process, ensuring that the cosmic essence remains intact. It's a ritual that honors the plant's journey, preserving its cosmic magic for us to enjoy. As we eagerly await the cosmic transformation, let us express our gratitude to the celestial forces that have guided us on this cosmic odyssey. The cosmos has aligned to bring us the extraordinary Cosmos F1, a gift from the cosmic garden gods themselves. We extend our heartfelt appreciation to the cosmic harmony orchestrated by @James and Royal Queen Seeds. In the coming days, our dried cosmic buds will be ready for the next stage of our journey. From cosmic concoctions to cosmic culinary delights, the possibilities are infinite. Imagine infusing the cosmic power of CBD into soothing teas, cosmic creams, or cosmic confections. The universe is our canvas, and with our cosmic harvest, we can create cosmic masterpieces. As we bid farewell to our Cosmos F1, let us cherish the cosmic memories we've shared. The vibrant colors, the pure CBD power, and the cosmic connection we've established with the universe. May our future endeavors be as cosmic and awe-inspiring as this one. Until we meet again, my cosmic friends, lets keep exploring the cosmic wonders, nurturing our cosmic gardens, and let the cosmic energy guide our cosmic creations. May our harvest be abundant, our cosmic experiences be enlightening, and our cosmic journey be filled with celestial blessings. As always thank you all for stopping by and for supporting me on this journey, i am super passion about growing and fell blessed to have you all with me on
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🍀🍀🍀🍀🍀🍀🍀🍀🍀🍀
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@Thomas86
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Raised Ec to 1.7, the banana punch is further along than the Cherry and has some trichs on the leaves already despite the buds being in a early stage, the Cherry Cola continues to grow in height and overtook the other this week, I recalibrated my Ph/Ec Pen to ensure accuracy. humidity is quite high at the moment but I expect it to drop where I live in the next few weeks hopefully! 3 plants in the 80x80cm tent has the humidity higher than id like but the plants seem happy enough for now. The 3 are guzzling the water, Not sure exactly how much but I topped up the reservoir by at least 30 liters this week.
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Pfff, explicaros que estas green ak xL son una variedad bastante difícil de cultivar, los cambios ambientales le sientan fatal, y hay que tener cuidado con la alimentación ya que se Sobrefertiliza fácilmente así que ojo, eso por un lado. Por otro si eres un cultivador con varios años de experiencia y todo te sale bien, es probarla, porque el sabor de esta cepa compensa la dificultad de su cultivo. Thc hasta 18% , sativa predominante. Genéticas: Afghan x Colombian x Mexican x Thai Landrades.
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@Dunk_Junk
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I cannot believe how much growth this week!!! I think she has doubled in size!! For the first time I've had to higher the light fixture. It is now almost as high as I can put it. I'm kinda regretting not FIMing her now...... I hope she does not grow much more in height!
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Flush Success!!! Budz on Budz! Everything is Beastly and Beautiful at thier 2nd week of Flower! 2nd Week of Flower: 2nd Flower Feed of #HumboldtCountysOwn and the Ladies Love the Bloom portion of Nutrients! They're Stacking Up Great and Filling Out thier Mighty Branches with Giant Gorgeous Budz! 5 Confirmed Females!!! It's been a few days since I gave the Ladies thier 2nd Feed of #HumboldtCountysOwn Nutrients and they look Fantastically Divine!!! We'll definitely Grow back to everything here soon! 11th w/ Veg and Flowers Update #Pre-Sexed🚺 #SuperCropped #Defoliated #Lollipoped Good Day and Happy Growing Everyone! These Ladies were Growing into thier 11th Week of Veg and Looking Totally Tight and Phenomenally *Femed* from the Right! #Pre-Sexed 4/9/21 #Opium/#PabloEscobar *Fems* breed by Team #DivineSeeds #DivineSeedsSquad #DivineSeedsBreedingCompany and #SuperCropping continued! I'm Very Excited and Extremely Gracious!!! Heck Yeah Growmies and Growmets!!! Check Out these Confirmed Females with thier Wild Pistol Hairs popping and I'm still Training and Widening them Out with #SuperCropping Everything looks Expanded and Growing Great! After #SuperCropping the plant will Grow Bushier, Produce More Buds, and possibly even Produce More THC! This is how the plant naturally responses to danger and protects itself! Growers take advantage of this technique to get Bigger Yields and More Potent Buds than would be produced otherwise! Getting Trained out into thier 11th Week of Veg and Looking Mighty Fine!!! Thier getting Humongous and Hearty in thier 10 gallon Grow Bag from Broski #www.as420.ca These Cannabis plants are Camping out in the Closet for a week or so! Lol This is my 2nd run using the Osree.Light QB1000w and Everything is Mesmerizing!!! My #Opium and #PabloEscobar *Fems* from #DivineSeeds Regs: SalamiLegF3's×2 and FireAlienRomulan look$ Outstandingly Awesome and Growing Up Phenomenally Fine into thier 11th week of Veg in our 2- 10 gallon The Grow Bags from Broski #www.as420.ca and also 2- 5 gallons as Well! Our others are Super and Breed by #thehumancannabiniod #AlienBloodGenetix #TNBNaturals #Foop Yo!!! Thanks Again for All the Love and Support my fellow Growmies and Growmets!!! These Magnificent and Magical Ladies were Growing in our 48×24×60 ViparSpectra Official Club 🌿 ®️ Grow Tent and under our #Osree QB1000w and #GrowStarStore Kokokala QB1000w and our Purple Rose Cutting×2, Aloe Vera Cutting, Maters×3, Morel Mushroom, Lavender×3, Sunflowers×5 and The 5 Brand New and Fresh Starts are Wonderful with a Huge Hint of Fantastical Fire is my #SalamiLegF3×2 by Broski #thehumancannabiniod #DankFlowGenetics #EastCoastSeedBank #FireAlienRomulan by Broski #AlienBloodGenetix my #PabloEscobar and #Opium by Team #DivineSeeds #DivineSeedsBreedingCompany We Grow with #Growatt 600w with #FoopOrangicBioSciences and #TNBNaturals The Enhancer Co2 Despersal canister and ph'd them w/ Declorinated watering w/ TNB ph Up and Down! Totally Top Quality Topz all Around! Thanks Again #GallowGlas420 #HarleyGrower #thehumancannabiniod #AlienBloodGenetix #TNBNATURALS #FoopOrangicBioSciences #www.as420.ca #divineseeds #divineseedssquad #divineseedsbreedingcompany #BioFlux Family's and Teams!!! Your Genetics are Merry Magnificent, Mega Marvelous and Mighty Medical! Love um!!! I really Can't stress enough how Thankful and Grateful I am of All of you and what you've Shared with Us! Means the World!!! It's definitely a Pleasure being able to Grow with these Phenomenal Grow Lights! Please Enjoy! BudBrothers4-Life! Cheers Famz!!! Much Props and Much Topz!💯🔥 #Osree #GrowStarStore #Growatt #TNBNaturals #TNBTeam #FoopOrangics #GrowYourOwn #Bliss HumboldtCounty'sOwn: Email: [email protected] Phone: (707) 725-4119 Toll free: (866) 872-9434 Humboldt Bottling Po Box 429 · Fortuna, CA 95540 HumboldtCounty'sOwn Website Link: https://humboldtcountysown.com/ HumboldtCounty'sOwn Instagram Link: https://instagram.com/humboldtcountysown?igshid=1qj809qkkwych https://instagram.com/osree.light?igshid=1vrsbdyhzg2h8 https://instagram.com/growstarstore?igshid=2yjn0r00r6ru https://instagram.com/divine.seeds?igshid=1r90iwe9xiwz8 https://instagram.com/thehumancannabinoid?igshid=17k159pumklkq https://www.instagram.com/alienblood_genetix_official/ https://instagram.com/as420.ca?igshid=1f116alw054wp The Grow Bags: https://www.as420
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@MrStryker
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Well it’s was there final week of Veg !!! I gave straight PhD water this last week and she loved it hadn’t missed a beat gave it a nice defoliation/lollipop headed into this first week of bloom ensuring it doesn’t get to congested as the may double or triple in size these strains I chose are all small plants at finish and all finish in 55-63 days of flower so not looking forward to a 9 plant trim at harvest but excited to see what these girls have in store going forward #HappyGrowing Everyone
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So far, so good. Smells already fantastic
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|They are loooking so good!!! https://www.youtube.com/watch?v=hGQ5Lx4-Quw Watch the video here!
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Hola chicos aqui mostrando el procesos de esta jamaican que van super sanitas esperando con ancias no mas esos racimos. Saludos gente...!!!
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~ WEDDING CHEESECAKE FAST FLOWER by FastBuds ~ Well fam, here we go again with another epic strain from FastBuds Fast Flowering stable. After having such tremendous success growing their Gorilla Cookies Fast Flower outdoors last year, I've decided to run another of their fast flowering strains outdoors this year... Wedding Cheesecake Fast Flower! The best description of this awesome cultivar comes directly from my friends at FastBuds which is as follows: "A delight for the mind and body: Wedding Cheesecake FF (Fast-Flowering) is a delicious strain that offers XXL yields in a 7-8 weeks flowering cycle. This terpene powerhouse produces loads and loads of mouth-watering vanilla-creamy-cookie-scented terps with hints of citrus and cinnamon. In addition to its delicious aromas, this variety grows very well in both indoor and outdoor setups, yielding up to 650 g/m2 without extra maintenance, making it the ideal strain for beginners as it allows you to maximize yields while minimizing work. This super-fast feminized photoperiod version offers upbeat and energetic yet deeply relaxing effects that are perfect for consumers looking for a heavily focused, motivating, and creative high. It’s the ideal strain for those needing that extra energy boost to start a busy day on the right foot. Wedding Cheesecake FF boasts chunky, large-sized buds with a thick layer of silvery-white resin and bright orange hairs scattered all over: that just screams ‘quality’. The lime-green flowers sparkle with an array of hues ranging from dark to light green, to magenta and purple, and reek of a delicious aroma that’ll remind you of fresh store-bought cheesecake. This is an XXL hybrid that grows extremely tall in height, reaching up to 3m with one huge main cola and multiple long side branches that can withstand enormous yields of up to 650 g/m2 with ease. Wedding Cheesecake FF is extremely resilient to the elements and can be grown in almost every climate as long as you’ve got the space and basics covered, making her an excellent choice for those looking for large-scale harvests with a quick turnaround time without much effort." ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ The Setup: This is going to be an outdoor grow, but I have started the Wedding Cheesecake FF indoors as our weather is still too cold to put her outside (nighttime temp's dipping regularly into the 30's℉). The plan is simple... let her grow inside under a 19/5 light schedule until the nighttime temperatures stay above the mid 40's℉, at which point she'll be moved outside and transplanted into the soil which I have already setup and inoculated with beneficial microbes, and then let the fun begin!🤪💚 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Weekly Updates: 6/18- Week 12 and the Wedding Cheesecake Fast Flower from FastBuds is on a mission! The amount of growth happening right now can only be described as explosive, with new shoots appearing and existing branches going crazy with growth! 6/20- Our summer is here and we're under an early heat wave this week with daytime temp's hitting the mid to upper 90's ℉ with heat indexes in the low 100's ℉... in short, IT'S FNG HOT!🔥🔥🔥 I'm watering the Wedding Cheesecake FF daily now with 5-7g of straight well water from the garden hose. I'm watering at sundown so that the water will have some time to soak down into the soil before the heat evaporates it. The coating of straw that I previously spread over the soil has helped a lot as well. 6/22- The weather continues to relentlessly bake everything outside... many thanks for a good A/C!❄️ The daily watering continues and I also went ahead and removed a bunch of foliage that was below the trellis net. 6/24- Today I top dressed the FastBuds Wedding Cheesecake FF with 2 cups each of Gaia Green 4-4-4 and 2-8-4 along with 1 cup of Down To Earth Bio-Fish 7-7-2 and 1/2g of Wiggle Worm castings. After top dressing and working the amendments in by hand I replaced the straw coating over the soil and watered them in with 5g of straight well water via garden hose as usual. There goes another week for the Wedding Cheesecake FF and it won't be long before she begins to show signs of pre-flowering! Thank you for checking out my diary, your positive comments and support make it all worthwhile! 💚Growers Love!💚😎🙏
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ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.
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Altough the "anonymous" seedling was still a bit behind, I decided to move the plants into the AutoPots. The pots were still disconnected from the tank and the plants were handwatered for the time being. 3 days later it was clear that "anonymous" had died. I was getting a bit frustrated since the fourth pot was now empty and the other 3 plants were growing happily. I started another germination attempt.
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I went ahead and transferred this week I also decided to start up my out door project things are looking good clones are Adapting well to new systems