with no agitation, that'll happen quickly. try to use it 24-48hours.. and if it's warm, stick to 24.. if concerned could pH test it... possible microbes could have eaten up some nutes too. you'd need a tds reading before to know on that.
best answer : Maybe... but is it worth trying to find out or risking your plants? microbes can quickly cause a dangerous pH range in addition to potential for underfeeding if they leeched any nutes.
if your nutes are super expensive, think about a brand change. quality fertilizer is cheap. You can go as low as 4-5cents per gallon mixed. Everything over that is profit margin and shipping water weight around.