2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Started LST on AG#5, transplanted to 5 gal pots on day 49 & hung Scorpion R-Spec dimmed to 200 watts. New Luko dehumidifier in place.

Greetings to any helpful advice

Looking amazing, this grow has been great overall.

Just checking the trichome heads daily to harvest them at the for me perfect moment.

This week she grew 23cm taller! I have been doing experiments through the week FIMing various upper growth sites. We will see what effects this will have in the weeks to come.. I've also been seeing upper leaf tips going a very light green. Is it nutrient burn? Light burn? Nutrients used this week: 20 grams 20-20-20 powder mixed with 10 litres of water + 40ml of Cannazym = ~1470ppm Timelapse sequence is: Auto Jack *** Pineapple express *** CBD Lime Blue Dream Auto Jack *** Pineapple Express *** CBD Lime Blue Dream

I pour the beet by Hand. Every two days like 5%-10% of the total soil amount. Today (12.5.) is 28.Day of flower (55-70 Days total) Lamps are on 600w the top of the flowers have 600-800ppfd Vpd is 1.2-1.3

🌿💋 Otra semanita más con nuestra Kiss de @kannabiaglobal y sigue poniéndose guapa 💋🌿 La Kiss está sacando su lado más dulce, gente. Los cogollos se están poniendo cada vez más gorditos y llenos de resina, y el aroma que empieza a soltar ya es una locura. Un toque dulce con fondo herbal que te dan ganas de cosecharla ya, pero aún le daremos un poquito más de amor. 🌱✨ Esta semana se ha notado cómo las flores están apretándose y cubriéndose de tricomas. Se ve saludable, vibrante y sigue mostrando ese verdor intenso. Cada día más cerca de la recompensa, ¡esto promete! 🙌🔥 #KissCannabis #KannabiaGlobal #SeguimientoDeCultivo #Floracion #CannabisGro

Flowers week 10 Flush week

Day 60F The PM on the cheese has gotten significantly worse in the last few days. I think it may have something to do with the constant humidity spikes every time I water. I haven’t been running my humidifier at all in flower so maybe it’s having trouble keeping the RH up during lights on. Just another reason to follow a daily watering schedule. The cheese and slurricane look fucking disgusting and I’m starting to doubt that a peroxide wash can totally fix it. It worked well for my outdoor crop so it should be fine but this time there is probably 3x the amount. I’ll make sure to really soak the buds and rinse them off good. Bbb#1 is flopping over like crazy from the weight. Too late to stake it up now so hopefully it holds until next week. Starting to foxtail a bit but nothing too bad. It is now 100% done and ready for chop. Bbb#2 is just getting frostier and frostier by the day. The purple fade is really coming along nicely too. Might reveg. It is now 100% done and ready for harvest. Black garlic #1 is finally filling out! I can’t believe it. Never heard of a plant packing weight on like that in week 8. The breeder did mention that it packs on weight later but I’m stunned. Taking her to 9.5 weeks. Black garlic #2 is looking very average. Average color, average size buds, average frost, average smell, probably an average yield. And it’s starting to get a bit of PM. Overall not happy. Will be taking her to 9.5 weeks. Zkittlez #1 has pleasantly surprised me. It was a bushy runt in veg but came through. It’s the third frostiest in the tent, super dense, stacked tight, smells great and looks great. It’s already got a nice amount of amber so it’s 100% ready for chop. Slurricane #4 looks insanely frosty even under all that PM. If I can’t wash it off it’s going in the trash. I want to chop this asap but have to cut everything at once unfortunately. It’s 100% ready for harvest. Badazz OG cheese is looking like a decent yielder but doesn’t have much else going for it. I think it still has some filling out to do. I can’t even smell the final profile change yet. The leaves are rotting off very quickly. Taking her 9.5 weeks. I don’t mind harvesting a bit later rather than early. From my outdoor experience, when a plant is picked early it smells like hay and wet grass clippings until it’s been curing for 3 weeks. When I harvest late or at 30-50% amber trichomes it smells like same scent profile it had when growing. Just my experience. I’ve read that the tobacco industry adds calcium and potassium carbonate to their crops to help promote fire holding capacity and to increase the whiteness of ash. Turns out some of the organic amendments I bought last week contain exactly that. I’m going to give each plant a light top dress to see if the tobacco industry is onto something.

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Keeps going. Etsy seeds coming through. I think soon in the grow a cal mag problem comes up. Chime in when you see it.

8 Days 60 55-60 Hum, 65-70 temps

Venimos muy bien con todas las plantas a pesar de esta ola de calor que no se quiere ir. Por un lado las fancy están en su etapa de lavado para ser cortadas próximamente. Las colombian Jack vienen flotando muy vigorosamente, y la kritikak k se está bancando el calor del exterior como puede

Getting settled into the tent and the 5 gallon pot. Getting used to tent settings, added a humidifier.

I love this new diary app! Getting to document each and every grow, makes it easier to improve the next time. If this website continues to gain more and more growers, I think Grow Diaries might be the goto site for uniting together growers from all over the world.

Week 3 and 2 days since the repot into 15ltr pots Looking beautiful atm and healthy. Just wish they would grow quicker. Starting to lst now. Any advice will be used.

Grown 23cm this week! Entered flowering also!