The earlier you stick them in the better their root system will be especially for an auto. after a transplant give about 1 week before doing another just to give them time for their roots to go out.. I usually transplant out of my solo cups after about two weeks putting them in from my peat pods.. A tip i learned is their root system is about as wide as their leaves.. when the leaves hit the edge of your solo up that's about the time! Happy growing!