That's teh right time to do it... myco that matters only survives with roots... so having them colonize early will have the best resutls.
some level of phosphate, i believe or potassium, does likely kill or severely reduce the myco... This is why it's doubley useless in a soilless/hydro context - soilless uses hydro nutes which is 100% plant available and ready to use, so no need for anything to be altered or processed by myco or microbe for the plant as it is in soil with certain forms of fertilizer and soil amendments that require such things before a plant can use it.
So, a little early... then maybe put some more at transplant. Don't need much early, but maybe a bit more later.. pop it all around where the plug will reside.. perfect placement for new roots to be colonized. Once colonized the rest just happens. even if you didn't asdd some at transplant, it'd probably fill in on it's own, but if you dolled out instructed amount for size of pot, it'll be good... just use a small bit of that dose early. the rest later... no OD / waste,,, assuming instructions are proper relative to size of pot used. each product has different spore counts, so it'l vary as to what dose you need for "optimal", whatever that may be. just math...