Good week same as last time couple more tears, some more major then others. But the original first snap seems to be healed so im confident with tapping they would heal fast. Going to be cutting for clones because i want to flip into flower ASAP going ti transplant into 5gallon first. Also ordering a new LED. Amazon led did okay for the budget but its time to see what i can really do! Very excited 🤠

Plants took a hard hit with bad ph levels in week 4 for a few days but are growing strong again and I will probably veg till the end of week 6 on these girls and switch to flowering in week 7. Overall it’s going good, water temps are a steady 18 Celsius with the thermoelectric water cooler I built and the small ac unit In the tent is holding the air temps between 23-25 Celsius. I added 4 gal of fresh water and nutrients at full strength to the reservoir on Day 38. Also the first lower screen was installed and I tied down/tucked a few of the larger branches to the screen.

2/10: I watered today with about 3/4 gallon each, plus cal-mag, signal, bembe, armor si, a little open sesame, and their final dose of endoboost. I took photos today, but forgot to snap a shot of the Soooperrunt. She's as tall as the short one now, just not as bushy. I think she'll make a fair amount of bud despite her sloooow start, smaller stature, and smaller pot. The tall one is keeping pace with the biggest plants in the garden. 2/11: Wife home sick today....postponing construction project to raise the lights....kinda scary....about 10"-12" from the tops now.. 2/12: I woke up to find the tallest of these bitches stretched another few inches and within 9 inches of the lights!!! I quickly drug everybody out of the closet and undertook the project. In addition to that project, I installed and hooked up my new AC Infinity 6" intake fan. It's pulling in fresh air from the soffit vent on the eave of the attic, and currently feeding the garden with 46f fresh air. I'm able to easily maintain daytime temps in the lower 70f's now. I am able to drive the nighttime temps as low as I want. The only issue is that the outdoor RH varies quite a bit, so I ordered a 30-pint dehumidifier to put in the top of the closet. It will battle with the evaporative cooler while the lights are on to keep it at 45% RH, but after the lights go off, it will lower the RH to 35% unti morning. After another couple of weeks, I'll kill the evap cooler altogether and try to maintain 35% RH 24/7. I'm optimistic that it will be the difference-maker in maintaining lower RH while I'm flushing all the plants during the last couple of weeks. That's usually such a challenge...especially with a bunch of 5 gallon pots. I also sprayed everybody with boomboom spray to try and mitigate the light burn damage that is likely to ensue. 2/13: Still stretching... about 12" from the lights again. I will wait to raise the lights until tomorrow when I feed them. I'm seeing calcium deficiency on a few plants, including a #9. Will up the dose tomorrow when I feed. 2/14: I fed them today with about 3/4 gallon each including grow big, big bloom, tiger bloom, cal-mag, signal, bembe, humic acid, and I switched over from Open Sesame to Beastie Bloomz. Raised the lights another couple of inches. I did some training on them and defo'd a little bit. 2/15: Installed the new dehumidifier and rigged the continuous drain on it...works great. 2/16: I rotated the edge plants and removed some old leaves. I added another 22w 3000k 4' bar light under the canopy. 2/17: I rearranged the garden and defoliated a little bit. That's it for week 8-

I had such a good response applying raw aloe very gel to the silver haze, just look at this weeks pics compared to last weeks. You can clearly see the difference. Aloe is high in beneficial enzymes and growth factors and more importantly an excellent source of silica. Cannabis flower, specifically the trichomes are made up of between 78% and up to 86% silica depending on the cultivar, so far only a small number of cultivars have been tested. However its clear that cannabis flowers NEED silica. Silica will also help with drought tolerance, it will armour your plant against pests, it will help the plant with transpiration, it will make water transport much easier for the plant, freeing up energy for other things like flower production. Also, If used as a foliar spray once a week in veg, it will help tighten up node spacing. So if you want those frosty buds make sure you have silica readily available for your plants.

Semana 1 (18/7/22 - 24/7/22) Se preparo una solucion de Mills Start R: - PPM : 100 -pH : 5.8 Se submergio cubos de roca de lana dentro de la soulcion. Cada semilla que se germinara se paso a un cubo de roca de lana y se mantuvo sin luz. Cuando las plantulas salian del cubo, se pasaron a otra bandeja de germinacion con domo de humedad bajo una luz T5.

Nothing much tae say really a hope the triple g makes it to the end of ita cycle and the unexpected church seems tae be a bonus beautiful looking healthy plant with a strong orange zest coming off it will also have the pleasure of an early smoke win win

Inizio 4 settimana di fioritura 💪...gente è incredibile ma vero! Ho messo in terra un pezzo di piantina che mi s'è rotta(talea) ,senza radicante ne niente. Solo messa in terra...sono passati quasi 10 giorni ed è ancora viva e sta crescendo...vuol dire che ha attaccato con le radici.......non ci credevo nemmeno io, ma quando mi dicono che ho il pollice verde inizio a crederci😂🤣😉😂🤣💪✌️🕉️. W la ganja sempre.....la pianta sacra che salverà il mondo

DAY63 WEEK9 = Impatiently waiting till harvest time 😘 Beautiful to enjoy the plants ripening and finishing 😍😍😍 Congrats to BIOTABS, because their tablest are really superb 🙏 I'm super satisfied 😁😁😁 Wedding Gelato, Triple G, and Cookies Gelato are completely ripe.👍😃 All their buds are rockhard 💪😖 👉 Cookies Gelato have become completely purple with beautiful piled buds and orange pistils, and looks delicious. Smells of berries and 😵 👉 Wedding Gelato has round buds (easy to trim) with mixed colors : gold, brass, brown, yellow, red and green colors with orange pistils 😊 Fine and accessible smell, pleasant and no overbearing 😄 🙏 👉 Triple G will be the hardest to trim, because of her quirky shaped buds. She has big internodes and big buds scattered across her tall sturdy stalks. Beautiful buds, a pleasure for the eyes. Her smell is pungent, sometimes narcotic even. She will get you stoned in no time 😋 👉 Liberty Haze could have another week. But she lacks some nitrogen; watch her leaves turn yellow, and make her taste sooo soft 👻 She hasn't any purple or red colors, only green and yellow. Her buds are leafy, not that firm, but volumineus. She's soo sweet, with hints of lime. 😘 I defoliated the plants in week 3 because I had to treat the plants against spider mites. Because of this, the plants had fewer leaves, the light could penetrate much deeper and now the lower buds are ripen as well 🙏. I will continue to apply this technique in the future. 👍👍👍 These will be the last pictures before harvesting 😍

Eine Blue Zushi hat sich entschieden ein Hermi zu sein und musst das Zelt leider verlassen. 💛🏼

Coming along,.. roll on next week

Soaked seeds all in peroxide solution for effortless germination 48 hours or until root pops out 1mm, than I paper towel them for 2 days for tap roots before planting in the medium. Note: Smile now Cry Laterz and FX3 were started one week later. Not pictured in this post. The first 6 popped were lemon cherry pop, which are from S1 seeds I made from 2 females using the stress method. I just raised my flowering temps for one day at 92 degrees during week 4 flowering to make them herm as an experiment and it worked😅! This was during a hot summer and I just shut off the a/c for a day in the room which I usually keep at 77-82 degrees! So I basically tricked them!

I'm 90% sure this one is in her finishing week here. More amber trichromes are coming in daily. I don't wanna push her too far like last time because this strain turned pure amber and kinda lost some crystaly luster during the curing process. My light timer, grow tent, and light upgrades really made a big difference in bud development compared to my last run with Runtz basically leading to bigger more frostier buds. I'm dropping temps lower at night. I give it a couple liters a day to keep the top soil moist. Flushed water comes out 6.8 ish. My CO2 exhale bag was the biggest flop as it was expired and did not activate. I plan on 48 hours darkness before harvest starting today: the day of the eclipse. With the new moon we have a high pressure system that is peaking at night around 1028 dB. Water should be pushed up into the plant from roots. Coupled with lights out....all theoretical witch craft. I have a small 2x2 dark tent pictured which needs to have AC bring in cold fresh air from outside. If no fresh air added the tent gets humid and stagnant after a while. The darkness period is bringing out that strong and sweet 'medicinal' smell. If you ever tried weed labeled medicinal youd have an idea of what I mean. I guess thats the orange bud/chemo lineage traits. Before lights off the smell was more towards yeast fermentation smell and not soo sweet.

I'm now on vacation for 2 weeks so all photos will be from my security camera. From what I can tell my new clones are greening up and putting out some new growth. My blumats had a runaway on day 3... luckily my neighbor was around and was able to slow the drip rate down on my one plant which was starting to flood my box. It has now stopped dripping and my hope is there will be no more hiccups..I will be watching closely over my vacation. 😅🙏

A beautiful structure to this plant and complemented nicely by its unique smell. Shaping up to carry some football sized colas, really excited about this harvest. Beastie Bloom and a sweetener is being used for the next few weks. Buds are packing on weight and hardeneing up!!!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Sep 7: nice lemony smell happening and the buds are forming. Not a particularly fast flowering plant but it’s going good. Sep 8: got some time under the UV light this afternoon. Once the Blueberry Pie is done she’ll get more UV light hours but for now she has to wait her turn. Sep 12: cool nights starting to cause some purple colour. Nice smell to her, but really leafy and seems a bit slow. Watered with potassium silicate and cal-mag today.

Plants have been drinking water for 2 full weeks and are ready for harvest. I am trying to take photos with the HPS and it's so difficult. I may need to invest to a filter..

Babies are growing soooo fast. Very happy about this strain! Can't wait for a tasting session... 😉