Hi there! Great question about LST (Low Stress Training) technique. Generally speaking, you don't have to remove the clips when the stems have grown thick, but it might be a good idea. Here are a few points to consider:
If the clips aren't digging into the stem or restricting growth, you can leave them on.
However, removing the clips can prevent potential damage to the plant as it continues to grow.
If you decide to remove the clips, do so carefully to avoid injuring the plant.
After removing clips, you can use other support methods like soft ties if necessary.
Regularly check your plant and adjust or remove clips as needed.
Remember, the main goal of LST is to guide your plant's growth without stressing it, so listen to your plant's needs and act accordingly.